Spatially varying defocus map estimation from a single image based on spatial aliasing sampling method.

In current optical systems, defocus blur is inevitable due to the constrained depth of field. However, it is difficult to accurately identify the defocus amount at each pixel position as the point spread function changes spatially. In this paper, we introduce a histogram-invariant spatial aliasing sampling method for reconstructing all-in-focus images, which addresses the challenge of insufficient pixel-level annotated samples, and subsequently introduces a high-resolution network for estimating spatially varying defocus maps from a single image. The accuracy of the proposed method is evaluated on various synthetic and real data. The experimental results demonstrate that our proposed model outperforms state-of-the-art methods for defocus map estimation significantly.

Priors-assisted dehazing network with attention supervision and detail preservation.

Single image dehazing is a challenging computer vision task for other high-level applications, e.g., object detection, navigation, and positioning systems. Recently, most existing dehazing methods have followed a "black box" recovery paradigm that obtains the haze-free image from its corresponding hazy input by network learning. Unfortunately, these algorithms ignore the effective utilization of relevant image priors and non-uniform haze distribution problems, causing insufficient or excessive dehazing performance. In addition, they pay little attention to image detail preservation during the dehazing process, thus inevitably producing blurry results. To address the above problems, we propose a novel priors-assisted dehazing network (called PADNet), which fully explores relevant image priors from two new perspectives: attention supervision and detail preservation. For one thing, we leverage the dark channel prior to constrain the attention map generation that denotes the haze pixel position information, thereby better extracting non-uniform feature distributions from hazy images. For another, we find that the residual channel prior of the hazy images contains rich structural information, so it is natural to incorporate it into our dehazing architecture to preserve more structural detail information. Furthermore, since the attention map and dehazed image are simultaneously predicted during the convergence of our model, a self-paced semi-curriculum learning strategy is utilized to alleviate the learning ambiguity. Extensive quantitative and qualitative experiments on several benchmark datasets demonstrate that our PADNet can perform favorably against existing state-of-the-art methods. The code will be available at https://github.com/leandepk/PADNet.

A three-stage deep learning-based training frame for spectra baseline correction.

For spectrometers, baseline drift seriously affects the measurement and quantitative analysis of spectral data. Deep learning has recently emerged as a powerful method for baseline correction. However, the dependence on vast amounts of paired data and the difficulty in obtaining spectral data limit the performance and development of deep learning-based methods. Therefore, we solve these problems from the network architecture and training framework. For the network architecture, a Learned Feature Fusion (LFF) module is designed to improve the performance of U-net, and a three-stage training frame is proposed to train this network. Specifically, the LFF module is designed to adaptively integrate features from different scales, greatly improving the performance of U-net. For the training frame, stage 1 uses airPLS to ameliorate the problem of vast amounts of paired data, stage 2 uses synthetic spectra to further ease reliance on real spectra, and stage 3 uses contrastive learning to reduce the gap between synthesized and real spectra. The experiments show that the proposed method is a powerful tool for baseline correction and possesses potential for application in spectral quantitative analysis.

Multi-Class Wound Classification via High and Low-Frequency Guidance Network.

Wound image classification is a crucial preprocessing step to many intelligent medical systems, e.g., online diagnosis and smart medical. Recently, Convolutional Neural Network (CNN) has been widely applied to the classification of wound images and obtained promising performance to some extent. Unfortunately, it is still challenging to classify multiple wound types due to the complexity and variety of wound images. Existing CNNs usually extract high- and low-frequency features at the same convolutional layer, which inevitably causes information loss and further affects the accuracy of classification. To this end, we propose a novel High and Low-frequency Guidance Network (HLG-Net) for multi-class wound classification. To be specific, HLG-Net contains two branches: High-Frequency Network (HF-Net) and Low-Frequency Network (LF-Net). We employ pre-trained models ResNet and Res2Net as the feature backbone of the HF-Net, which makes the network capture the high-frequency details and texture information of wound images. To extract much low-frequency information, we utilize a Multi-Stream Dilation Convolution Residual Block (MSDCRB) as the backbone of the LF-Net. Moreover, a fusion module is proposed to fully explore informative features at the end of these two separate feature extraction branches, and obtain the final classification result. Extensive experiments demonstrate that HLG-Net can achieve maximum accuracy of 98.00%, 92.11%, and 82.61% in two-class, three-class, and four-class wound image classifications, respectively, which outperforms the previous state-of-the-art methods.

Prediction of Impulsive Aggression Based on Video Images.

In response to the subjectivity, low accuracy, and high concealment of existing attack behavior prediction methods, a video-based impulsive aggression prediction method that integrates physiological parameters and facial expression information is proposed. This method uses imaging equipment to capture video and facial expression information containing the subject's face and uses imaging photoplethysmography (IPPG) technology to obtain the subject's heart rate variability parameters. Meanwhile, the ResNet-34 expression recognition model was constructed to obtain the subject's facial expression information. Based on the random forest classification model, the physiological parameters and facial expression information obtained are used to predict individual impulsive aggression. Finally, an impulsive aggression induction experiment was designed to verify the method. The experimental results show that the accuracy of this method for predicting the presence or absence of impulsive aggression was 89.39%. This method proves the feasibility of applying physiological parameters and facial expression information to predict impulsive aggression. This article has important theoretical and practical value for exploring new impulsive aggression prediction methods. It also has significance in safety monitoring in special and public places such as prisons and rehabilitation centers.

A hybrid coded-aperture and Compton camera based on cerium-doped Gd3Al2Ga3O12 scintillators coupled with multi-pixel photon counter arrays.

In this study, we propose a hybrid coded-aperture and Compton camera based on cerium-doped Gd3Al2Ga3O12 (GAGG:Ce) scintillator arrays coupled with Multi-Pixel Photon Counter (MPPC) arrays. The sensitive detector of the gamma camera consists of a single GAGG:Ce crystal coupled with a single-chip MPPC unit module. An impedance bridge circuit and a 64-channel data acquisition system were employed to record the code-aperture events and Compton coincidence events. After the calibration of position and energy, the total energy resolution for 662 keV gamma-rays from 137Cs was 6.6%. The hybrid camera had the characteristics of mechanical collimation and electronic collimation at the same time. In the code aperture mode, the reconstructed images were obtained by direct deconvolution and maximum likelihood expectation maximization (MLEM) methods. In the Compton imaging mode, the energy-dependent method was applied to order the sequence of Compton scatter events. The simple back-projection algorithm and list-mode MLEM algorithm were adopted for image reconstruction. Practical performances demonstrated that the angular resolutions in two modes were measured as 5.2° and 11.4°, respectively. In addition, the hybrid camera had a desirable imaging capability in a wide energy range (32 keV-2.6 MeV) and a wide field of view (∼210° in the horizontal direction). As for the sensitivity, the camera had a commercially available sensitivity level of localizing a 137Cs point source, producing ∼0.026 µSv/h in 5 min. Furthermore, the function of distinction for different radiation sources was preliminarily realized.

[Low-dose Spiral Computed Tomography in Lung Cancer Screening].

Lung cancer is one of the malignant tumors with the highest morbidity and mortality in the world. The low early diagnosis rate and poor prognosis of patients have caused serious social burden. Regular screening of high-risk population by low-dose spiral computed tomography (LDCT) can significantly improve the early diagnosis rate of lung cancer and bring new opportunities for the diagnosis and treatment of lung cancer. In recent years, LDCT lung cancer screening programs have been carried out in many countries around the world and achieved good results, but there are still some controversies in the selection of screening subjects, screening frequency, cost effectiveness and other aspects. In this paper, the key factors of LDCT lung cancer screening, screening effect, pulmonary nodule management and artificial intelligence contribution to the development of LDCT will be reviewed, and the application progress of LDCT in lung cancer screening will be discussed.
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Rational selection of RGB channels for disease classification based on IPPG technology.

The green channel is usually selected as the optimal channel for vital signs monitoring in image photoplethysmography (IPPG) technology. However, some controversies arising from the different penetrability of skin tissue in visible light remain unresolved, i.e., making the optical and physiological information carried by the IPPG signals of the RGB channels inconsistent. This study clarifies that the optimal channels for different diseases are different when IPPG technology is used for disease classification. We further verified this conclusion in the classification model of heart disease and diabetes mellitus based on the random forest classification algorithm. The experimental results indicate that the green channel has a considerably excellent performance in classifying heart disease patients and the healthy with an average Accuracy value of 88.43% and an average F1score value of 93.72%. The optimal channel for classifying diabetes mellitus patients and the healthy is the red channel with an average Accuracy value of 82.12% and the average F1score value of 89.31%. Due to the limited penetration depth of the blue channel into the skin tissue, the blue channel is not as effective as the green and red channels as a disease classification channel. This investigation is of great significance to the development of IPPG technology and its application in disease classification.

Optimized Golay-9 array configurations for mid-frequency compensation in optical sparse aperture systems.

Optical sparse aperture (OSA) imaging systems show great potential to generate higher resolution images than those of equivalent single filled aperture systems. However, due to the sparsity and dispersion of sparse aperture arrays, pupil function is no longer a connected domain, which further attenuates or loses the mid-frequency modulation transfer function (MTF), resulting in lower mid-frequency contrast and blurred images. Therefore, an improved traversal algorithm is proposed to optimize Golay-9 array configurations for compensating the mid-frequency MTF. Its structural parameters include diameters of sub-apertures, relative rotation angles between individual sub-apertures, and radius of concentric circles. Then, these parameters are traversed successively in order. Finally, the influences of the obtained optimized array configurations on the mid-frequency MTF are analyzed in detail, and the image performances are evaluated. The experimental results prove the contrast enhancement. Compared with a Golay-9 array at F=36.5%, the maximum MTF increases from 0.1503 to 0.307, and the mid-frequency MTF is boosted from 0.0565 to 0.0767. In addition, the peak signal to noise ratio of the degraded image is promoted from 19.75 dB to 20.63 dB. Both quantitative and qualitative evaluations demonstrate the validity of the proposed method.

MTF improvement for optical synthetic aperture system via mid-frequency compensation.

Optical synthetic aperture imaging system has grown out the quest for higher angular resolution in astronomy, which combines the radiation from several small sub-apertures to obtain a resolution equivalent to that of a single filled aperture. Due to the discrete distribution of the sub-apertures, pupil function is no longer a connected domain, which further leads to the attenuation or loss of the mid-frequency modulation transfer function (MTF). The mid-frequency MTF compensation is therefore a key focus. In this paper, a complete mid-frequency compensation algorithm is proposed, which can extract and fuse the frequency of different synthetic aperture systems and monolithic aperture systems according to their special MTF characteristics. The dimensions of the monolithic aperture and optical synthetic aperture system are derived, and the longest baseline of the monolithic aperture is much smaller than that of the optical synthetic aperture system. Then the separated spatial frequency information is extracted and synthesized according to the spatial frequency equivalence point. Finally, the full-frequency enhanced image is recovered by using improved Wiener-Helstrom filter, which adopts specific parameters based on different sub-aperture arrangements. The mid-frequency MTF of Golay-3 increases from 0.12 to 0.16 and that of Golay-6 increases from 0.06 to 0.18. Both the simulation and experiment prove that the proposed method not only realizes the spatial resolution determined by the longest baseline of the optical synthetic aperture system, but also successfully compensates its mid-frequency MTF.

Breadth-first piston diagnosing approach for segmented mirrors through supervised learning of multiple-wavelength images.

Piston diagnosing approaches for segmented mirrors via machine-learning have shown great success. However, they are inevitably challenged with 2π ambiguity, and the accuracy is usually influenced by the location and number of submirrors. A piston diagnosing approach for segmented mirrors, which employs the breadth-first search (BFS) algorithm and supervised learning strategies of multi-wavelength images, is investigated. An original kind of object-independent and normalized dataset is generated by the in-focal and defocused images at different wavelengths. Additionally, the segmented mirrors are divided into several sub-models of binary tree and are traversed through the BFS algorithm. Furthermore, two deep image-based convolutional neural networks are constructed for predicting the ranges and values of piston aberrations. Finally, simulations are performed, and the accuracy is independent of the location and number of submirrors. The Pearson correlation coefficients for test sets are above 0.99, and the average root mean square error of segmented mirrors is approximately 0.01λ. This technique allows the piston error between segmented mirrors to be measured without 2π ambiguity. Moreover, it can be used for data collected by a real setup. Furthermore, it can be applied to segmented mirrors with different numbers of submirrors based on the sub-model of a binary tree.

A camera-based ballistocardiogram heart rate measurement method.

Recent studies have shown that head movements associated with cardiac activity contain a heart rate (HR) signal. In most previous studies, subjects were required to remain stationary in a specific environment during HR measurements, and measurement accuracy depended on the choice of target in the scene, i.e., the specified region of the face. In this paper, we proposed a robust HR measurement method based on ballistocardiogram (BCG) technology. This method requires only a camera and does not require that users establish a complex measurement environment. In addition, a bidirectional optical flow algorithm is designed to select and track valid feature points in the video captured by using the camera. Experiments with 11 subjects show that the HR values measured using the proposed method differ slightly from the reference values, and the average error is only 1.09%. Overall, this method can improve the accuracy of BCG without limitations related to skin tone, illumination, the state of the subject, or the test location.

Object-independent piston diagnosing approach for segmented optical mirrors via deep convolutional neural network.

Piston diagnosing approaches based on neural networks have shown great success, while a few methods are heavily dependent on the imaging target of the optical system. In addition, they are inevitably faced with the interference of submirrors. Therefore, a unique object-independent feature image is used to form an original kind of data set. Besides, an extremely deep image-based convolutional neural network (CNN) of 18 layers is constructed. Furthermore, 9600 images are generated as a data set for each submirror with a special measure of sensitive area extracting. The diversity of results among all the submirrors is also analyzed to ensure generalization ability. Finally, the average root mean square error of six submirrors between the real piston values and the predicted values is approximately 0.0622λ. Our approach has the following characteristics: (1) the data sets are object-independent and contain more effective details, which behave comparatively better in CNN training; (2) the complex network is deep enough and only a limited number of images are required; (3) the method can be applied to the piston diagnosing of segmented mirror to overcome the difficulty brought by the interference of submirrors. Our method does not require special hardware, and is fast to be used at any time, which may be widely applied in piston diagnosing of segmented mirrors.

Image restoration for synthetic aperture systems with a non-blind deconvolution algorithm via a deep convolutional neural network.

Optical synthetic aperture imaging systems, which consist of in-phase circular sub-mirrors, can greatly improve the spatial resolution of a space telescope. Due to the sub-mirrors' dispersion and sparsity, the modulation transfer function is decreased significantly compared to a fully filled aperture system, which causes obvious blurring and loss of contrast in the collected image. Image restoration is the key to get the ideal clear image. In this paper, an appropriative non-blind deconvolution algorithm for image restoration of optical synthetic aperture systems is proposed. A synthetic aperture convolutional neural network (CNN) is trained as a denoiser prior to restoring the image. By improving the half-quadratic splitting algorithm, the image restoration process is divided into two subproblems: deconvolution and denoising. The CNN is able to remove noise in the gradient domain and the learned gradients are then used to guide the image deconvolution step. Compared with several conventional algorithms, scores of evaluation indexes of the proposed method are the highest. When the signal to noise ratio is 40 dB, the average peak signal to noise ratio is raised from 23.7 dB of the degraded images to 30.8 dB of the restored images. The structural similarity index of the results is increased from 0.78 to 0.93. Both quantitative and qualitative evaluations demonstrate that the proposed method is effective.

Effect of FOXK2 overexpression on malignant biological behaviors of ovarian cancer SK-OV-3 cells / 中国肿瘤生物治疗杂志

@#[Abstract] Objective: To investigate the effects of forkhead box transcription factor (FOXK2) overexpression on the proliferation, migration, invasion and adhesion of human ovarian cancer SK-OV-3 cells and its related molecular mechanism. Methods: The open reading frame (ORF) of FOXK2 was cloned into lentivirus expression vector, which was then enveloped in HEK293T cells and transfected into human ovarian cancerSK-OV-3cells.TheoverexpressionefficiencywasdetectedbyqPCRandWesternblotting.Theproliferation, migration, invasion and adhesion of SK-OV-3 cells were detected by CCK-8, Scratch-healing, Transwell and Cell adhesion assays respectively, and the expressions of epithelial-mesenchymal transition (EMT) markers were detected by qPCR. Results: The FOXK2 overexpression vector was constructed successfully and packaged into lentivirus, which was then transfected into SK-OV-3 cells. After transfection, the expression of FOXK2 was significantly increased (P<0.01); the proliferation, migration and invasion of SK-OV-3 cells were significantly reduced while the adhesion ability was significantly increased (P<0.05 or P<0.01); and the expression levels of E-cadherin and β-catenin were significantly increased while that of vimentin and fibronection were significantly decreased (all P<0.01). Conclusion: Overexpression of FOXK2 in SK-OV-3 cells leads to a significant decrease in proliferation, migration and invasion but increase in adhesion. The molecular mechanism may be related to the reversion of the EMT process in tumor cells, suggesting that FOXK2 may be a potential target for the diagnosis and treatment of ovarian cancer.

Hybrid imaging with extended depth of field based on binocular ranging.

We present a new method to reduce the image artifacts in defocused hybrid imaging systems with a cubic phase mask. Image artifacts are caused by a mismatch of phase during decoding. A new definition of the Strehl ratio is proposed, and the defocus tolerance of the high-quality decoded image is calculated using this standard. According to the corresponding relationship between the defocus amount and the object distance, we analyze the feasibility of identifying the defocus amount according to distance information obtained by the binocular ranging principle to optimize the decoding process via simulation. Experimental results show that our method can improve image quality over a wide range of defocus.

Increasing aperture and depth of field simultaneously with wavefront coding technology.

We present a method that simultaneously increases the aperture and depth of field (DOF). A novel, to the best of our knowledge, method called lens-combined wavefront coding (WFC) is proposed for optical design. By rationally balancing rather than minimizing the aberrations, the DOF enhances instead of reduces with expansion of the aperture size. Two optical systems by traditional design and lens-combined WFC are designed to demonstrate our concept. Experiments are conducted using the manufactured lenses to show the extension of the DOF both in the laboratory and the real scene. A spatially invariant deconvolution algorithm is exploited to further suppress the aberrations regarding the field of view. The results show that the aperture and the DOF can be successfully enhanced at the same time.

Edge detection for optical synthetic apertures based on conditional generative adversarial networks.

Detecting the interference fringes of the optical synthetic aperture is the core in preventing misalignments of the sub-mirrors in piston, tip, and tilt. These fringes are characterized as follows: (1) the edge information of sub-mirrors is accompanied by complex shapes and large gaps; and (2) the traditional edge detection algorithms have different optimal thresholds under different interference fringes, and they may lose boundary information. To address these problems, a novel method for detecting the edge of synthetic aperture fringe images is proposed. Because conditional generative adversarial networks avoid the difficulty of designing the loss function for specific tasks, they are suitable for our project. We trained over 8000 images based on real images and simulated images. Experiments prove that the proposed method can reduce the false detection rate to 0.2, compared with 0.56 by Canny algorithm. This method can also directly detect the fringe edge of the optical synthetic aperture systems, which are accompanied by varied shapes and a growing number of sub-mirrors. When the input images lose boundary information, the traditional algorithm does not restore the boundary, but the proposed method makes a decision globally, and thus it guesses and then fills the boundary. The maximum error of the generated boundary and the actual boundary is two pixels.

Robust real-time heart rate prediction for multiple subjects from facial video using compressive tracking and support vector machine.

Remote monitoring of vital physiological signs allows for unobtrusive, nonrestrictive, and noncontact assessment of an individual's health. We demonstrate a simple but robust image photoplethysmography-based heart rate (HR) estimation method for multiple subjects. In contrast to previous studies, a self-learning procedure of tech was developed in our study. We improved compress tracking algorithm to track the regions of interest from video sequences and used support vector machine to filter out potentially false beats caused by variations in the reflected light from the face. The experiment results on 40 subjects show that the absolute value of mean error reduces from 3.6 to 1.3 beats/min . We further explore experiments for 10 subjects simultaneously, regardless of the videos at a resolution of 600 by 800, the HR is predicted real-time and the results reveal modest but significant effects on HR prediction.

Differential computation method used to calibrate the angle-centroid relationship in coaxial reverse Hartmann test.

A differential computation method is presented to improve the precision of calibration for coaxial reverse Hartmann test (RHT). In the calibration, the accuracy of the distance measurement greatly influences the surface shape test, as demonstrated in the mathematical analyses. However, high-precision absolute distance measurement is difficult in the calibration. Thus, a differential computation method that only requires the relative distance was developed. In the proposed method, a liquid crystal display screen successively displayed two regular dot matrix patterns with different dot spacing. In a special case, images on the detector exhibited similar centroid distributions during the reflector translation. Thus, the critical value of the relative displacement distance and the centroid distributions of the dots on the detector were utilized to establish the relationship between the rays at certain angles and the detector coordinates. Experiments revealed the approximately linear behavior of the centroid variation with the relative displacement distance. With the differential computation method, we increased the precision of traditional calibration 10-5 rad root mean square. The precision of the RHT was increased by approximately 100 nm.