Acupoint nanocomposite hydrogel for simulation of acupuncture and targeted delivery of triptolide against rheumatoid arthritis.

BACKGROUND: Attenuating inflammatory response and relieving pain are two therapeutic therapeutical goals for rheumatoid arthritis (RA). Anti-inflammatory and analgesic drugs are often associated with many adverse effects due to nonspecific distribution. New drug delivery systems with practical targeting ability and other complementary strategies urgently need to be explored. To achieve this goal, an acupoint drug delivery system that can target deliver anti-inflammatory drugs and simulate acupuncture in relieving pain was constructed, which can co-deliver triptolide (TP) and 2-chloro-N (6)-cyclopentyl adenosine (CCPA). RESULTS: We have successfully demonstrated that acupoint nanocomposite hydrogel composed of TP-Human serum album nanoparticles (TP@HSA NPs) and CCPA could effectively treat RA. The result shows that CCPA-Gel can enhance analgesic effects specifically at the acupoint, while the mechanical and thermal pain threshold was 4.9 and 1.6 times compared with non-acupoint, respectively, and the nanocomposite gel further enhanced. Otherwise, the combination of acupoint and nanocomposite hydrogel exerted synergetic improvement of inflammation, bone erosion, and reduction of systemic toxicity. Furthermore, it could regulate inflammatory factors and restore the balance of Th17/Treg cells, which provided a novel and effective treatment strategy for RA. Interestingly, acupoint administration could improve the accumulation of the designed nanomedicine in arthritic paws (13.5% higher than those in non-acupoint at 48 h), which may explain the better therapeutic efficiency and low toxicity. CONCLUSION: This novel therapeutic approach-acupoint nanocomposite hydrogel, builds a bridge between acupuncture and drugs which sheds light on the combination of traditional and modern medicine.

Occurrences, Retention and Risk Assessments of PAHs in Beidagang Wetland in Tianjin, China.

Coastal wetlands are the last barriers for pollutants from land to the sea. In this study, a coastal wetland that locates in the lower reach of Haihe River Systems was selected to speculate the removal and retention of polycyclic aromatic hydrocarbons (PAHs) by analyzing their spatial distributions and the changes of composition. The results showed that the overall removal efficiency of PAHs in water phase was 58.1%. There was an accumulation for sedimentary PAHs, reaching 431 ng/g (181 ng/g in the inlet). The compositions of sedimentary PAHs were also changed, high-molecular-weight PAHs were the main component (70-50%), with a steady decreasing trend and the influence of water flow direction. The risk assessment by mean effect range media quotients (M-ERM-Qs) depicted that there was in low ecological risk, due to the degradation of PAHs in the wetlands. Our results clearly demonstrated the coastal wetlands could effectively retain the PAHs, thus we recommend an active protection strategy for the coast wetlands in Tianjin in the future.