Removal of remodeling/reprogramming factors from oocytes and the impact on the full-term development of cloned embryos.

The reason for the poor development of cloned embryos is not yet clear. Several reports have suggested that some nuclear remodeling/reprogramming factors (RRFs) are removed from oocytes at the time of enucleation, which might cause the low success rate of animal cloning. However, there is currently no method to manipulate the amount of RRFs in oocytes. Here, we describe techniques we have developed to gradually reduce RRFs in mouse oocytes by injecting somatic cell nuclei into oocytes. These injected nuclei were remodeled and reprogrammed using RRFs, and then RRFs were removed by subsequent deletion of somatic nuclei from oocytes. The size of the metaphase II spindle reduced immediately, but did recover when transferred into fresh oocytes. Though affected, the full-term developmental potential of these RRF-reduced oocytes with MII-spindle shrinkage was not lost after fertilization. When somatic cell nuclear transfer was performed, the successful generation of cloned mice was somewhat improved and abnormalities were reduced when oocytes with slightly reduced RRF levels were used. These results suggest that a change in RRFs in oocytes, as achieved by the method described in this paper or by enucleation, is important but not the main reason for the incomplete reprogramming of somatic cell nuclei.

Subcutaneous angiolipoma: magnetic resonance imaging features with histological correlation.

PURPOSE: Despite producing pain, angiolipoma is sometimes misdiagnosed as an ordinary small lipoma, which is usually not associated with pain. Few reports have described magnetic resonance (MR) imaging findings of angiolipoma. The aim of the present study was to clarify the MR imaging features of angiolipoma. MATERIALS AND METHODS: The MR imaging findings of 11 lesions in 7 patients were reviewed and compared with histopathological findings. RESULTS: The MR imaging features of these lesions were the presence of fat nodules with or without areas of low signal intensity on T1- and T2-weighted images. The location of the low-signal-intensity areas varied. The low-signal-intensity areas were mainly in the peripheral portion of 3 lesions and in the central portion of 5 lesions. No or few low-signal-intensity areas were observed in 3 lesions. In the lesions with peripheral low-signal-intensity areas, lesion marginations were well defined, and the lesions were easily recognized as mass lesions. In the lesions with only central low-signal-intensity areas, marginations were poorly defined, and the lesions were not easy to recognize as mass lesions. In the lesions with few or without low-signal-intensity areas, marginations were invisible. Histopathological studies indicated that the low-signal-intensity areas on T1- and T2-weighted images corresponded to areas of dense capillary proliferation. In lesions with few or without low-signal-intensity areas on MR images, capillaries were thinly spread over almost the entire lesion area. CONCLUSION: The MR imaging features of angiolipoma are fat nodules with or without low-signal-intensity areas of various size and location on T1- and T2-weighted images.

Recurrence of fibrous hamartoma of infancy excised 14 years after the primary surgery.

Fibrous hamartoma of infancy is a rare, benign, superficial soft-tissue mass. It usually occurs within the first 2 years of life at the axial regions, upper arms, and external genital areas. There have been some recurrences within the 1st year of the surgery, although no cases have been reported to recur after 1 year. The authors present a recurrent case of fibrous hamartoma of infancy 14 years after the primary surgery, and they show the clinical and histopathological findings.

Clinical usefulness of assessing lumbar somatosensory evoked potentials in lumbar spinal stenosis. Clinical article.

OBJECT: The aim of this retrospective study was to evaluate the clinical usefulness of assessing lumbar somatosensory evoked potentials (SSEPs) in central lumbar spinal stenosis (LSS). METHODS: The latencies of lumbar SSEPs were recorded in 40 patients with central LSS, including 16 men and 24 women. The mean age of the patients was 67.3 +/- 7.4 years. The diagnosis was LSS in 23 cases and LSS associated with degenerative spondylolisthesis in 17 cases. The average duration of symptoms was 43.8 +/- 51.2 months. Twenty-two cases had bilateral and 18 cases had unilateral leg symptoms. Thirty-seven cases were associated with neurogenic intermittent claudication and the mean walking distance of patients with this condition was 246.8 +/- 232.7 m. The mean Japanese Orthopedic Association scale score, as well as the visual analog scale (VAS) scores of low-back pain, leg pain, and numbness, were 16.5 +/- 3.5, 6.0 +/- 2.5, 6.9 +/- 2.1, and 7.8 +/- 2.2, respectively. The minimal cross-sectional area of the dural sac on MR imaging was 0.44 +/- 0.21 cm(2). Thirty-nine cases of cervical spondylotic myelopathy without lumbar and peripheral neuropathy were chosen as the control group. RESULTS: The latencies of lumbar SSEPs in patients with LSS and in the control group were 23.0 +/- 2.0 ms and 21.6 +/- 1.9 ms, respectively. There was a statistically significant difference between the LSS and control groups (p < 0.05). The latency of lumbar SSEPs was significant correlated with the VAS score of leg numbness (p < 0.05). The latency of lumbar SSEPs in LSS was clearly delayed when the VAS score of leg numbness was > or = 8 (p < 0.05). CONCLUSIONS: Lumbar SSEPs are able to detect neurological deficit in the lumbar area effectively, and they can reflect part of the subjective severity of sensory disturbance (numbness) in LSS. Both lumbar SSEPs and VAS scores of leg numbness may be useful for clinical evaluation in patients with LSS.

Complexation of alpha-cyclodextrin with carborane derivatives in aqueous solution.

Although the complexation of carborane derivatives with beta-cyclodextrin (beta-CD) is well-known, we present here the first observation of the complexation of carborane derivatives with alpha-CD in aqueous solution. The stoichiometry and association constant (K(a)) of the complexes were estimated from Job's plots and NMR titration studies, respectively. The carborane : alpha-CD stoichiometry was 1 : 1 in all cases. The complexation ability and selectivity of the carborane derivatives for alpha-CD are markedly decreased compared with those for beta-CD. The interaction between the carborane cage and the hydrophobic cavity of alpha-CD appears to be weak, probably because the hydrophobic cavity of alpha-CD is too small to accommodate the carborane cage. The C-H hydrogen and the polar substituents of carborane cage may form hydrogen bonds with secondary alcohol groups at the rim of alpha-CD. The orientation of the carborane cage influences the inclusion process, and o- and m-carborane derivatives showed moderately stronger association constants than p-carborane derivatives.

Clinical value of motor evoked potentials with transcranial magnetic stimulation in the assessment of lumbar spinal stenosis.

The objective of this study was to evaluate the clinical usefulness of assessing motor evoked potentials (MEP) in lumbar spinal stenosis (LSS). Twenty-three LSS patients were enrolled. The preoperative data of MEP latency (MEPLT), clinical symptoms, Japanese Orthopaedic Association (JOA) scores for low back pain, visual analogue scale (VAS) for back pain, leg pain and numbness, walking distance and the minimal cross-sectional area (mCSA) of the dural sac were evaluated. The mean MEPLT was 42.1 +/- 2.8 ms. Fourteen patients had bilateral leg symptoms. The mean walking distance and mCSA were 302.1 +/- 302.8 m and 0.4 +/- 0.2 cm(2), respectively. The mean JOA score and VAS scores for back pain, leg pain and numbness were 15.9 +/- 4.8, 6.0 +/- 2.9, 7.7 +/- 1.9 and 7.3 +/- 3.0, respectively. MEPLT was related to the walking distance, limb symptoms and the VAS for numbness. MEPLT was significantly delayed in patients who showed a walking distance less than 500 m. MEP is useful in LSS assessment. It can reflect the subjective severity of motor disturbance and predict the neurological deficit prior to appearance.

New mathematical approach for approximating the baseline of F-waves using spreadsheet software.

OBJECTIVE: The aim of this study was to see if curved baselines of F-waves could be mathematically approximated with universal spreadsheet software. METHODS: The subjects were 3 healthy persons and 3 patients with cervical myelopathy. Supramaximal electrical stimuli were applied 200 times to the median nerve at the wrist. Compound muscle action potentials (CMAPs) of the abductor pollicis brevis were recorded. To make polynomial approximation equations that represent latter part of the M-waves, records without F-waves were analyzed. RESULTS: There were 193 CMAPs without F-waves out of all 1,200 records. Polynomial equations were made for each record. Determinant coefficients for all the approximation equations were greater than 0.998, and the overall standard deviation of the difference between original data and approximated value was 3.05 microV. CONCLUSIONS: Curved baselines of F-waves were represented by approximation curves. Baselines of the F-waves could be approximated as flat lines by subtracting calculated values from the original data. SIGNIFICANCE: This method was useful for analyzing waveforms of F-waves.

Epidemiological study of low back pain and occupational risk factors among taxi drivers.

A survey of taxi drivers was conducted to determine the actual situation of drivers' low back pain (LBP). The survey was carried out in October 2002, the target drivers were asked to complete a questionnaire which contains questions regarding physique of drivers, demographic features, working conditions, office environment, health conditions, the presence of low back pain, the level of low back pain based on Visual Analogue Scale and Roland-Morris Disability Questionnaire score. As a result, the total number of valid responses was 1,334 and the response rate was 71 percent, and the 1-wk prevalence of LBP was 20.5 percent of respondents. Regarding 275 subjects with LBP, Visual Analogue Scale (VAS) averaged 4.3. There was a positive weak correlation between VAS and Roland-Morris Disability Questionnaire score (R=0.41). And Logistic regression analysis was performed to examine the relationship between LBP and occupational factors, the results suggested following items as risk factors; such as history of LBP, suffering from fatigue, diseases other than LBP and smoking habit.