[Metabolic state of erythrocytes under gall-stone disease]. / Metabolicheskoe sostoianie éritrotsitov pri zhelchno-kamennoi bolezni.

Metabolic state of erythrocytes is changed under gall-stone disease. The increase of glucose and glycated hemoglobin contents in erythrocytes is combined with intensification of glycolytic reactions and lowered activity of pentose phosphate pathway. These changes may have compensative importance.

[Haemoglobin system of Black Sea round goby under experimental hypoxia conditions]. / Gemoglobinovaia sistema chernomorskogo bychka-krugliaka v usloviiakh éksperimental'noi gipoksii.

Haemoglobin of the Black Sea round goby (Neogobius melanostomus P.) resisting to external hypoxia was studied. Haemoglobin of this species was fractionated into 4 components (2 minor and 2 major ones) by the disk-electrophoresis in polyacrylamid gel. The major fractions were eluted and their respiration parameters were investigated. One of them showed high affinity for O2 (P50 5.81 +/- 0.74 mm Hg at pH 8.3) and heightened sensibility to pH (Bohr effect amounted to -0.81 +/- 0.09 at pH of 8.3 --> 7.5) simultaneously. Moreover, an acidosis incubation medium (pH 8.3 --> 7.5) decreased its O2 saturation by 12.8 +/- 1.4% (Root effect). The level of this fraction increased by 41.8% (p < 0.001) under the experimental hypoxia conditions (seawater O2 concentration range was 1.7-1.8 mg 1(-1); exposition--10 days). It affected blood respiration parameters and reflected the process of adaptation of this species to oxygen deficiency. The nucleotidtriphosphate level in red blood cells did not change. Swelling of circulated erythrocytes was observed simultaneously.

[Effect of polyunsaturated fatty acid-containing food additives on erythrocyte enzyme activities and on hemoglobin affinity to oxygen in highly and low trained volleyball players during physical exertion]. / Vplyv biologichno aktyvnykh kharchovykh dobavok, shcho mistiat' polinenasycheni zhirni kysloty, na aktyvnist' deiakykh vnutrishn'oerytrotsytarnykh fermentiv ta na sporidnenist' gemoglobinu do kysniu u sportsmeniv pry intensyvnomu fizychnomu navantazhenni.

It has been determined that in erythrocytes of high-qualified and low-qualified sportsmen-volleyboll after use of biopreparation "Polien", contained polyunsaturated fatty acids, glycolysis and pentose phosphate pathway reactions are intensified and glutathione reductase activity are decreased. At the same time the affinity of haemoglobin to oxygen in stabilized in organism of sportsmen before and after intensive muscle work. It has been shown, that specific and nonspecific changes of biochemical indexes are realized in organism of sportsmen with different qualification under intensive muscle work.

[Effect of "polyen" on modified serum albumin in athletes under physical load]. / Vliianie "poliena" na modifitsirovanie syvorotochnogo al'bumina u sportsmenov v usloviiakh fizicheskoi nagruzki.

It has been shown that during adaptation to muscle loads in organism of sportsmen serum albumin liganding becomes more intensive (particular by POL products), the protein effective concentration reduces and the formation of protein modificated form. After use of biopreparation "Polyen" containing polyunsaturated fatty acids w3, the ligand redistribution is realized, the conformational state of albumin is stabilized.

[Structure-activity features of serum albumin in athletes under physical load]. / Strukturo-funktsional'nye osobennosti syvorotochnogo al'bumina u sportsmenov pri vozdeistvii fizicheskoi nagruzki.

By circular dichroism the secondary structure of serum albumin has been studied in organism of high-qualified volleyball-sportsmen. It has been determined that changes in percentage correlation of alpha- and beta-structures of sportsmen serum albumin were combined with decrease of protein reserve functional activity and with its intensified liganding by substance of lipid and carbohydrate nature.

[Effect of the biopreparation "Polyene" on parameters of blood fatty acid composition in volleyball athletes]. / Vliianie biopreparata "polien" na pokazateli zhirnokislotnogo sostava krovi u sportsmenov-voleibolistov.

Influence of the preparation "Polyen" has been studied on indexes of lipid acids contents in blood plasma, erythrocyte membrane and serum albumin of high-qualified volleyball-players. It was shown that "Polyen" has favourable effect on different metabolic pathways of lipid acids, that allow to recommend this preparation as additional component in food ration for training sportsmen.

[The effect of biological food supplements containing polyunsaturated fatty acids on the erythrocyte enzyme activity and on the hemoglobin oxygen affinity in volleyball players exposed to intensive physical loading]. / Vliianie biologicheskikh pishchevykh dobavok, soderzhashchikh polinenasyshchennye zhirnye kisloty, na aktivnost' nekotorykh éritotsitarnykh fermentov i srodstvo gemoglobina k kislorodu u sporstmenov-voleibolistov pri vozdeistvii intensivnykh fizicheskikh nagruzok.

It has been determined that in erythrocytes of professional volley-ball players given biopreparation "Polien" containing polyunsaturated fatty acids glycolysis and pentose phosphate pathway reactions are intensified and glutathione reductase activity decreased. At the same time the affinity of haemoglobin to oxygen stabilized in the sportsmen before and after intensive muscle load.

[Lipid composition of blood plasma and erythrocyte membrane of volleyball players under intensive physical load]. / Lipidnyi sostav plazmy krovi i membran éritrotsitov u voleibolistov v usloviiakh intensivnoi fizicheskoi nagruzki.

As the result of prolonged training activity highly skilled volleyball players have marked deficiency of fatty acids of w3 family both in the blood plasma as erythrocyte membranes. These acids may be used in the lipid peroxidation processes, that is proved by the facts of POL products content and their drawing into oxidation reaction, which is certified by high level of ATP in erythrocytes. Reduction of quantity of polyunsaturated fatty acids of w3 family may be regarded as results of their insufficient arrival with food.

[The indices of intraerythrocyte metabolism and hemoglobin oxygen affinity in volleyball players under intensive physical loads]. / Pokazateli vnutriéritrotsitarnogo metabolizma i srodstvo gemoglobina k kislorodu u sportsmenov-voleibolistov pri intensivnykh fizicheskikh nagruzakh.

The authors' investigations led them to the conclusion that skilled volleyball players' erythrocytes undergo adaptive metabolic rearrangement. There was also low hemoglobin affinity to oxygen. A single training load provoked the effect of metabolic enhancement as a result of increased red cell concentration of glucose, glycosylated hemoglobin and high activity of hexokinase.

[Various physico-chemical properties of hemoglobins in representatives of different classes of vertebrates]. / Nekotorye fiziko-khimicheskie svoistva gemoglobinov u predstavitelei razlichnykh klassov pozvonochnykh.

The level of hydrophobic property and microviscosity in central and peripheral sections of molecules of main electrophoretic fractions of hemoglobins in representatives of different classes of vertebrates has been studied by means of the methods of fluorescent spectroscopy. It has been found that central sections of hemoglobin molecules are characterized by less pronounced changes in the volume and level of hydrophobic properties and highly pronounced changes in the packing integrity as compared to peripheral sections of the protein globule. Peripheral sections of hemoglobin molecules in the mammals are characterized by higher amount of surface tyrosines and lower level of hydrophobic properties against similar indices in representatives of other classes of vertebrates. Microviscosity (packing compaction) in central sections of hemoglobin molecules decreases with a transfer from low phylogenetic groups of vertebrates to higher ones.

[The species characteristics of the intramolecular structure of hemoglobin in vertebrates]. / O vidovykh osobennostiakh vnutrimolekuliarnoi struktury gemoglobina pozvonochnykh.

Using fluorescence spectroscopy species peculiarity of hydrophobic and microviscosity degree in central and peripheral molecular areas of the main electrophoretic haemoglobin fractions in representatives of different vertebrate classes gas been studied. It is established that central areas of haemoglobin molecule are characterised by twice lesser variability of size and hydrophobic degree and 2.4 times greater variability of package strength as compared with peripheral areas of protein globule.

[The comparative characteristics of the physicochemical and structural-functional properties of hemoglobin in a number of vertebrates]. / Sravnitel'naia kharakteristika nekotorykh fiziko-khimicheskikh i strukturno-funktsional'nykh svoistv gemoglobina v riadu pozvonochnykh.

Using isoelectrofocusing within pH gradient 6-8, studies have been made on microheterogeneity of haemoglobins from various vertebrates. Phylogenetic evaluation of oxygen affinity of the main and minor electrophoretically pure haemoglobin fractions is given. It was shown that oxygen affinity of the haemoglobins is related to the ratio between dicarboxylic and basic amino acids.

[Functional properties of blood proteins in highly trained athletes]. / K voprosu o funktsional'nykh svoistvakh belkov krovi u vysokotrenirovannykh sportsmenov.

The functional properties of serum albumin and hemoglobin were investigated in highly trained athletes. During adaptation to regular strenuous exercise, the metabolic pathway of omega 3 fatty acids intensified and the content of lipid peroxidation products in serum albumin increased. Also, hemoglobin affinity for molecular oxygen decreased and ATP concentration in red blood cells increased.