RESUMO
Cancer vaccines using dendritic cells (DCs) have been shown to induce antitumor immunity and have recently been applied to non-immunogenic cancers, such as pancreatic cancer. In this study, we utilized DCs loaded with heat-treated tumor lysate (HTL-DC) as a vaccine in order to stimulate antitumor immunity in a murine pancreatic cancer model and compared them to DCs loaded with tumor lysate (TL-DC). The poorly immunogenic mouse ductal pancreatic cancer cell line PANC02 with syngeneic mouse strain C57BL/6 was used as a model. Inducible heat shock proteins (HSPs) were significantly increased in HTL (HSP70 and HSP90). Tumor size measurements indicated that HTL-DC induced stronger tumor suppression than unpulsed DC or TL-DC (43% reduction in tumor volume compared to control group). T cell proliferation assay and IFN-gamma ELISPOT assay showed that T cell activation increased in the following order: DCAssuntos
Células Dendríticas/imunologia
, Imunoterapia
, Neoplasias Pancreáticas/imunologia
, Extratos de Tecidos/uso terapêutico
, Animais
, Proliferação de Células
, Feminino
, Proteínas de Choque Térmico/fisiologia
, Interferon gama/metabolismo
, Camundongos
, Camundongos Endogâmicos C57BL
, Neoplasias Pancreáticas/terapia
, Baço/citologia
, Baço/metabolismo
, Linfócitos T/fisiologia
, Linfócitos T Citotóxicos/fisiologia
, Extratos de Tecidos/imunologia