RESUMO
In this study, an in-house constructed paper-based spectrophotometer is presented and demonstrated for detecting three organic dyes, namely, methylene blue, malachite green, and rhodamine B, and monitoring the efficiency of their removal from a wastewater sample with Sistan sand as a costless adsorbent. The compact design and light weight of this simple spectrophotometer delivered portability, with materials costing less than a dollar. Spectral analysis of the captured images was performed using free downloadable software from the Google Play store. The main experimental parameters affecting the efficiency of dye adsorption including pH, sorbent dosage, initial dye concentration, and contact time were investigated and optimized using the Taguchi design experimental method. Validation experiments were performed using a standard commercial bench-top spectrophotometer, and results were compared in terms of analytical performance, speed, and cost of analysis. The smartphone-based spectrometer was able to measure accurately, as confirmed using the commercial spectrometer, with enhanced sensitivity for methylene blue and rhodamine B. The combination of the high spectral accuracy of the paper-based spectrophotometer, together with sand as a readily accessible sorbent, enabled us to develop a powerful yet simple approach and tool for the removal and monitoring of dyes within wastewater samples, which is potentially available to everybody who owns a smartphone.
RESUMO
Hyperthermia treatment induced by magnetic mesoporous glasses has been applied as a potential therapeutic approach for bone defects due to malignant tumors. The objective of this study was to synthesize and characterize the structural and biological properties of magnetic bioactive glasses (BGs) for producing multifunctional materials. The effect of the addition of copper (Cu) to the bioactive glass composition was also evaluated. Fe BG and FeCu BG as magnetic mesoporous BGs, and Cu BG as mesoporous BG were synthesized and dried by template sol-gel method. Then the synthesized bioglasses were characterized and analyzed using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive electron disperse spectroscopy (EDS), Brunauer-Emmett-Teller (BET), and vibrating sample magnetometer (VSM). In addition, the antibacterial behavior, cytotoxicity assay (MTT test), proliferation assay of HUVEC cell assay, and bioactivity (ALP activity test) of the synthesized BGs were evaluated. The characterization results exhibited that the synthesized powders formed mesoporous glasses with nanoparticle morphology, good surface area, and magnetic properties. The synthesized BGs also demonstrated suitable biological behavior. The magnetic saturation of bioactive glasses was increased by the addition of copper oxide. A two-phase structure was observed for the magnetic glasses compared to the copper-containing glasses, thus making them suitable for drug delivery systems. The antibacterial behavior was found to be better for the Cu BG and Fe BG compared to the FeCu BG. However, the least amount of cytotoxicity was observed for the Fe BG and FeCu BG, compared to the Cu BG. In addition, the Fe-containing BGs compared with the control group showed a lack of HUVEC cell proliferation and angiogenesis motivation. From the ALP assay, higher bioactivity for the magnetic bioglasses in the presence of mesenchymal cells was found. From the results of this in vitro study, the Cu-containing magnetic bioglass (FeCu BG) could be considered as a new generation of magnetic glasses for inducing hyperthermia in treatment of bone defects due to malignant tumors. However, further in vitro and in vivo studies are required to confirm their applications in healing of bone defects and tissue engineering.
