The effect of physical exercise during competitions and in simulated conditions on hormonal-neurophysiological relationships in kickboxers.

K1-format kickboxing is a widely followed combat sport that requires intense physical exercise. However, research into the body's response to this type of combat is sparse. This study aims to assess the alterations in hormone levels and brain activity in elite kickboxers following an actual K1 bout and compare these changes with those observed in a control group engaged in a simulated fight exercise with a punchbag. The study included 100 male professional kickboxers, randomly divided into two groups: an experimental group (K1 fight) and a control group (simulated fight with a punchbag). Blood samples were obtained before and after exercise to evaluate testosterone (T) and cortisol concentrations (C). Concurrently, brain activity was recorded using quantitative electroencephalography (QEEG). After the activity in the experimental group mean testosterone level slightly, non-significantly decreased from 13.7 nmol/l to 12.4 nmol/l, while mean cortisol significantly (p < 0.001) increased from 313 to 570 nmol/l. In the control group after the exertion against a punchbag mean cortisol significantly (p < 0.001) increased from 334 to 452 nmol/l and testosterone increased non-significantly, from 15.1 to 16.3 nmol/l. In both groups, the testosterone/cortisol ratio (T/C ratio) showed significantly lower levels after the intervention (p < 0.001 and p < 0.032) in the experimental and control group respectively. The comparison of groups after exercise revealed significantly higher cortisol levels (experimental group x = 570 nmol/l; control group x = 452 nmol/l) and a significantly lower T/C ratio (experimental group x = 2.7; control group x = 3.9), (p = 0.001) in the experimental group. Significantly higher brain activity was found in selected leads after a bout (experimental group). Furthermore, in the experimental group, significant associations of weak to moderate strength were found between hormone fluctuations and selected areas of brain activity (p < 0.05). K1-format kickboxing induces a stress response, evident in the sharp changes in cortisol and testosterone levels. A notable observation was the inverse direction of changes in both hormones. Brain activity analysis indicated the potential influence of raised cortisol concentrations on specific brain areas. This study augments our understanding of the physiological responses during K1 kickboxing bouts and may inform the future evolution of this sport.

The Role of MicroRNAs in the Pathophysiology of Osteoarthritis.

Worldwide, osteoarthritis (OA) is the most common cause of joint pain in older people. Many factors contribute to osteoarthritis' development and progression, including secondary osteoarthritis' underlying causes. It is important to note that osteoarthritis affects all four tissues: cartilage, bone, joint capsule, and articular apparatus. An increasingly prominent area of research in osteoarthritis regulation is microRNAs (miRNAs), a small, single-stranded RNA molecule that controls gene expression in eukaryotes. We aimed to assess and summarize current knowledge about the mechanisms of the action of miRNAs and their clinical significance. Osteoarthritis (OA) is affected by the interaction between miRNAs and inflammatory processes, as well as cartilage metabolism. MiRNAs also influence cartilage cell apoptosis, contributing to the degradation of the cartilage in OA. Studies have shown that miRNAs may have both an inhibitory and promoting effect on osteoporosis progression through their influence on molecular mechanisms. By identifying these regulators, targeted treatments for osteoarthritis may be developed. In addition, microRNA may also serve as a biomarker for osteoarthritis. By using these biomarkers, the disease could be detected faster, and early intervention can be instituted to prevent mobility loss and slow deterioration.

The MicroRNAs in the Pathophysiology of Osteoporosis.

Globally, osteoporosis is the most common systemic skeletal disease. There are many factors that influence osteoporosis' development and progression. During the pathogenesis of this disease, bone turnover is imbalanced between resorption and the formation of bone tissue. A growing interest has been devoted to the role that microRNA (miRNA) plays in osteoporosis regulation. A microRNA (miRNA) is a group of small single-stranded RNA molecules involved in regulating gene expression in eukaryotic organisms. As microRNAs (miRNAs) are key regulators of gene expression and can modulate processes related to bone metabolism, they have become increasingly important for studying osteoporosis pathogenesis. The available research suggests that miRNAs play an important role in regulating processes associated with bone metabolism, especially by influencing bone resorption and synthesis. Furthermore, microRNAs can also serve as potential therapeutic targets for osteoporosis, besides being a rapid and specific biomarker.

The Use of Quantitative Electroencephalography (QEEG) to Assess Post-COVID-19 Concentration Disorders in Professional Pilots: An Initial Concept.

Announced by WHO in 2020, the global COVID-19 pandemic caused by SARS-CoV-2 has affected many people, leading to serious health consequences. These consequences are observed in the daily lives of infected patients as various dysfunctions and limitations. More and more people are suffering post-COVID-19 complications that interfere with or completely prevent them from working or even functioning independently on a daily basis. The aim of our study was to demonstrate that innovative quantitative electroencephalography (QEEG) can be used to assess cognitive function disorders reported after the COVID-19 pandemic. It is worth noting that no similar study has been conducted to date in a group of pilots. The QEEG method we used is currently one of the basic neurological examinations, enabling easy observation of post-COVID-19 changes in the nervous system. With the innovativeness of this technique, our study shows that the use of quantitative electroencephalography can be a precursor in identifying complications associated with cognitive function disorders after COVID-19. Our study was conducted on twelve 26-year-old pilots. All participants had attended the same flight academy and had contracted SARS-CoV-2 infection. The pilots began to suspect COVID-19 infection when they developed typical symptoms such as loss of smell and taste, respiratory problems, and rapid fatigue. Quantitative electroencephalography (QEEG), which is one of the most innovative forms of diagnostics, was used to diagnose the patients. Comparison of the results between the study and control groups showed significantly higher values of all measurements of alpha, theta, and beta2 waves in the study group. In the case of the sensorimotor rhythm (SMR), the measurement results were significantly higher in the control group compared to the study group. Our study, conducted on pilots who had recovered from COVID-19, showed changes in the amplitudes of brain waves associated with relaxation and concentration. The results confirmed the issues reported by pilots as evidenced by the increased amplitudes of alfa, theta, and beta2 waves. It should be emphasized that the modern diagnostic method (QEEG) presented here has significant importance in the medical diagnosis of various symptoms and observation of treatment effects in individuals who have contracted the SARS-CoV-2 virus. The present study demonstrated an innovative approach to the diagnosis of neurological complications after COVID-19.

Google Trends Analysis Reflecting Internet Users' Interest in Selected Terms of Sexual and Reproductive Health in Ukraine.

INTRODUCTION: The war in Ukraine has had adverse impacts on all areas of life, including health-related issues. Limited access to medical care increases the need to look for alternative sources of medical information. AIM: To analyze trends of Internet users' interest in sexual and reproductive health in Ukraine based on Google Trends. MATERIALS AND METHODS: The retrospective study was based on the analysis of terms related to sexual and reproductive health searched by Ukrainian Internet users. The tool used was Google Trends. The period from 1 January 2021 to 1 January 2023 was analyzed. The time variability in search growths and peaks was assessed from the perspective of two time intervals (before the war and during the war) using the chi-square test. RESULTS: Significant changes have been demonstrated in the interests of Internet users from Ukraine regarding selected issues of sexual and reproductive health during the ongoing war. Compared to the pre-war period, a marked increase was observed in active searches for terms such as "condoms" (p = 0.0081), "rape" (p = 0.0008), "syphilis" (p = 0.0136), "ovulation" (p = 0.0002) and "pregnancy test" (p = 0.0008). CONCLUSIONS: The conducted analysis clearly indicates an increased need for information regarding sexual and reproductive health among Ukrainian citizens during the ongoing armed conflict. The analysis of trends among Internet users interests can be a valuable source of knowledge for decision makers, including human rights organizations, regarding the scope and coordination of activities aimed at protecting the sexual and reproductive health of the inhabitants of Ukraine.

Preliminary Development of a Brainwave Model for K1 Kickboxers Using Quantitative Electroencephalography (QEEG) with Open Eyes.

K1 kickboxing fighting is characterised by high injury rates due to the low restrictions of fighting rules. In recent years, much attention has been paid to research on changes in brain function among athletes, including those in combat sports. One of the tools that are likely to help diagnose and assess brain function is quantitative electroencephalography (QEEG). Therefore, the aim of the present study was an attempt to develop a brainwave model using quantitative electroencephalography in competitive K1 kickboxers. A total of thirty-six male individuals were purposefully selected and then comparatively divided into two groups. The first group consisted of specialised K1 kickboxing athletes exhibiting a high level of sports performance (experimental group, n = 18, mean age: 29.83 ± 3.43), while the second group comprised healthy individuals not training competitively (control group, n = 18, mean age: 26.72 ± 1.77). Body composition assessment was performed in all participants before the main measurement process. Measurements were taken for kickboxers during the de-training period, after the sports competition phase. Quantitative electroencephalography of Delta, Theta, Alpha, sensimotor rhytm (SMR), Beta1 and Beta2 waves was performed using electrodes placed on nine measurement points (frontal: FzF3F4, central: CzC3C4, and parietal: PzP3P4) with open eyes. In the course of the analyses, it was found that the level of brain activity among the study population significantly differentiated the K1 formula competitors compared with the reference standards and the control group in selected measurement areas. For kickboxers, all results of the Delta amplitude activity in the area of the frontal lobe were significantly above the normative values for this wave. The highest value was recorded for the average value of the F3 electrode (left frontal lobe), exceeding the norm by 95.65%, for F4 by 74.45% and Fz by 50.6%, respectively. In addition, the Alpha wave standard value for the F4 electrode was exceeded by 14.6%. Normative values were found for the remaining wave amplitudes. Statistically significant differentiation of results, with a strong effect (d = 1.52-8.41), was shown for the activity of Delta waves of the frontal area and the central part of the parietal area (Fz,F3,F4,Cz-p < 0.001), Theta for the frontal area as well as the central and left parietal lobes (Fz,F3,F4-p < 0.001, Cz-p = 0.001, C3-p = 0.018; d = 1.05-3.18), Alpha for the frontal, parietal and occipital areas (for: Fz,F3-p < 0.001, F4-p = 0.036, Cz-p < 0.001, C3-p = 0.001, C4-p = 0.025, Pz-p = 0.010, P3-p < 0.001, P4-p = 0.038; d = 0.90-1.66), SMR for the central parietal and left occipital lobes (Cz-p = 0.043; d = 0.69, P3-p < 0.001; d = 1.62), Beta for the frontal area, occipital and central lobes and left parietal segment (Fz,F3-p < 0.001, F4-p = 0.008, Cz, C3, Pz, P3,P4-p < 0.001; d = 1.27-2.85) and Beta 2 for all measurement areas (Fz, F3, F4, Cz, C3, C4, Pz, P3, P4-p < 0.001; d = 1.90-3.35) among the study groups. Significantly higher results were shown in the kickboxer group compared to the control. In addition to problems with concentration or over-stimulation of neural structures, high Delta waves, with elevated Alpha, Theta and Beta 2 waves, can cause disorders in the limbic system and problems in the cerebral cortex.

The Influence of Various Hydration Strategies (Isotonic, Water, and No Hydration) on Hematological Indices, Plasma Volume, and Lactate Concentration in Young Men during Prolonged Cycling in Elevated Ambient Temperatures.

BACKGROUND: Increased internal body temperature during dehydration can be accompanied by water-electrolyte imbalances, higher levels of lactate during and after physical exertion, and changes in blood volume. Adequate hydration with carbohydrate-electrolyte fluids during physical activity can prevent dehydration and delay the onset of fatigue, allowing for proper biochemical and hematological reactions during exertion. A suitable drinking plan should consider the pre-exercise hydration level as well as the requirements for fluids, electrolytes, and substrates before, during, and after exercise. The objective of this study was to assess the impact of different hydration strategies (isotonic, water, and no hydration) on hematological indicators (hemoglobin concentration, hematocrit number, erythrocyte count, leukocyte count, and mean corpuscular volume) and lactate concentration during prolonged physical exertion in a high-temperature environment in young men. METHODS: The research method was quasi-experimental. The study involved 12 healthy men aged 20.6 ± 0.9 years, who were characterized by a body height (BH) of 177.2 ± 4.8 cm, a body mass (BM) of 74.4 ± 7.6 kg, a lean body mass (LBM) of 61.1 ± 6.1 kg, and a body mass index (BMI) of 23.60 ± 0.48. Measurements were taken of body composition and hematological and biochemical indicators. The main tests consisted of three series of tests separated by a one-week break. During the tests, the men performed a 120 min exercise with an intensity of 110 W on a cycle ergometer in a thermo-climatic chamber at an ambient temperature of 31 ± 2 °C. During exertion, the participants consumed isotonic fluids or water in an amount of 120-150% of the lost water every 15 min. The participants who exercised without hydration did not consume any fluids. RESULTS: Significant differences in serum volume were observed between the use of isotonic beverage and no hydration (p = 0.002) and between the use of isotonic beverage and water (p = 0.046). Immediately after the experimental exercise, hemoglobin values were significantly higher with no hydration than with water (p = 0.002). An even stronger significance of differences in hemoglobin was observed between no hydration and isotonic beverage consumption (p < 0.001). There was a statistically significant difference in the number of leukocytes between the consumption of isotonic beverage and no hydration (p = 0.006). CONCLUSIONS: Each active hydration strategy allows for a better maintenance of water-electrolyte homeostasis during physical exertion in a high-temperature environment, and isotonic beverage consumption had a greater impact on hydrating extracellular spaces with the smallest changes in hematological indicators.

The Use of Neurofeedback in Sports Training: Systematic Review.

Biofeedback training is a method commonly used in various fields of life, for example, in medicine, sports training or business. In recent studies, it has been shown that biofeedback, and neurofeedback, can affect the performance of professional athletes. Training based on the neurofeedback method includes exercising the brain waves. The aim of the article is to evaluate the influence of neurofeedback training on the physical fitness of professional athletes representing various sports disciplines, such as judo, volleyball and soccer. Based on 10 scientific papers from various sources, including PubMed, the latest research on neurofeedback and its impact on athletes has been reviewed. On the basis of the literature review from 2012 to 2022 on the neurofeedback method in sports training, it can be stated that this type of practice has a significant impact on physical fitness and sports performance. This review comprised 10 research studies with 491 participants in the neurofeedback groups, and 62 participants in the control group. Two reviewers independently extracted data and evaluated the quality of the studies utilising the PEDro scale. Properly planned and conducted neurofeedback training affects stimulation and improvement of many variables (reducing stress levels, increasing the ability to self-control physiological factors, enhancing behavioural efficiency and meliorating the speed of reaction to a stimulus).

An Attempt to Develop a Model of Brain Waves Using Quantitative Electroencephalography with Closed Eyes in K1 Kickboxing Athletes-Initial Concept.

BACKGROUND: Brain injuries are a common problem in combat sports, especially in disciplines such as kickboxing. Kickboxing is a combat sport that has several variations of competition, with the most contact-oriented fights being carried out under the format of K-1 rules. While these sports require a high level of skill and physical endurance, frequent micro-traumas to the brain can have serious consequences for the health and well-being of athletes. According to studies, combat sports are one of the riskiest sports in terms of brain injuries. Among the sports disciplines with the highest number of brain injuries, boxing, mixed martial arts (MMA), and kickboxing are mentioned. METHODS: The study was conducted on a group of 18 K-1 kickboxing athletes who demonstrate a high level of sports performance. The subjects were between the ages 18 and 28. QEEG (quantitative electroencephalogram) is a numeric spectral analysis of the EEG record, where the data is digitally coded and statistically analysed using the Fourier transform algorithm. Each examination of one person lasts about 10 min with closed eyes. The wave amplitude and power for specific frequencies (Delta, Theta, Alpha, Sensorimotor Rhythm (SMR), Beta 1, and Beta2) were analysed using 9 leads. RESULTS: High values were shown in the Alpha frequency for central leads, SMR in the Frontal 4 (F4 lead), Beta 1 in leads F4 and Parietal 3 (P3), and Beta2 in all leads. CONCLUSIONS: The high activity of brainwaves such as SMR, Beta and Alpha can have a negative effect on the athletic performance of kickboxing athletes by affecting focus, stress, anxiety, and concentration. Therefore, it is important for athletes to monitor their brainwave activity and use appropriate training strategies to achieve optimal results.

Comparison of Head Strike Incidence under K1 Rules of Kickboxing with and without Helmet Protection-A Pilot Study.

BACKGROUND: Kickboxing is a combat sport that encompasses various forms of competition. K1 kickboxing is conducted without any restrictions on the force of strikes, and the bout can end prematurely through a knockout. Headgear has been introduced in amateur kickboxing to safeguard the head. However, scientific studies have shown that despite their use, serious head injuries can still occur. The aim of this study was to evaluate the temporal structure of the bout by calculating the number of head strikes in K1 kickboxing bouts with and without headgear. METHODS: Thirty K1 kickboxing bouts were analyzed, with 30 participants included in the study. The fights were conducted according to the World Association Kickboxing Organization (WAKO) rules. The bouts consisted of three rounds of 2 min each, with a 1 min break between rounds. Sparring pairs were arranged according to weight categories. The first bouts were conducted without headgear, and two weeks later, the fights were repeated with WAKO-approved headgear. The number of head strikes was assessed retrospectively by analyzing video recordings of the bouts, categorizing strikes as hand or foot strikes, and differentiating between strikes that hit the head directly or indirectly. RESULTS: The results showed statistically significant differences between bouts with and without headgear in terms of the number of strikes to the head (p = 0.002), strikes directly to the head (p < 0.001), all hand strikes to the head (p = 0.001), hand strikes directly to the head (p = 0.003), and foot strikes directly to the head (p = 0.03). Higher values were observed in bouts with headgear. CONCLUSIONS: Headgear increases the probability of direct strikes to the head. Therefore, it is important to familiarize kickboxers with the use of headgear in their sport to minimize head injuries.

Matrix Metalloproteinases in Cardioembolic Stroke: From Background to Complications.

Matrix metalloproteinases (MMPs) are endopeptidases participating in physiological processes of the brain, maintaining the blood-brain barrier integrity and playing a critical role in cerebral ischemia. In the acute phase of stroke activity, the expression of MMPs increase and is associated with adverse effects, but in the post-stroke phase, MMPs contribute to the process of healing by remodeling tissue lesions. The imbalance between MMPs and their inhibitors results in excessive fibrosis associated with the enhanced risk of atrial fibrillation (AF), which is the main cause of cardioembolic strokes. MMPs activity disturbances were observed in the development of hypertension, diabetes, heart failure and vascular disease enclosed in CHA2DS2VASc score, the scale commonly used to evaluate the risk of thromboembolic complications risk in AF patients. MMPs involved in hemorrhagic complications of stroke and activated by reperfusion therapy may also worsen the stroke outcome. In the present review, we briefly summarize the role of MMPs in the ischemic stroke with particular consideration of the cardioembolic stroke and its complications. Moreover, we discuss the genetic background, regulation pathways, clinical risk factors and impact of MMPs on the clinical outcome.

MiniQEEG and Neurofeedback in Diagnosis and Treatment of COVID-19-Related Panic Attacks: A Case Report.

BACKGROUND: Both the global COVID-19 pandemic situation, as well as the current political situation in Eastern Europe may exacerbate anxiety and contribute to stress-related disorders such as panic disorder. Electroencephalography (EEG)-based neurofeedback provides both assessment of the subject's brainwave activity as well as the possibility of its therapeutic correction. It is possible that it can be implemented as an auxiliary treatment in panic disorders of different origin. The aim of this feasibility study was to demonstrate (both short- and long-term) effectiveness of neurofeedback therapy in a patient with previously diagnosed panic attacks, related to fear of COVID-19 infection. METHODS: We report the case study of a 47-year-old man affected by panic attacks, related to his profound, constant fear of COVID-19 infection and its sequelae. For the initial diagnostic workup, several clinical and research tools were used: 1. Baseline psychological exam; 2. Anxiety-targeted interview performed by miniQEEG therapist; 3. Analysis of previous clinical test results (EEG record/lab blood test); and 4. The miniQEEG exam (central strip recording Cz-C3-C4), The patient was subjected to regular EEG Neurofeedback sessions for two consecutive months. After completing the treatment, follow-up tests, as listed above were repeated immediately after completing the whole treatment program, as well as 1 and 2 years later. MiniQEEG results were compared with healthy control (age-matched male subject not affected with panic attacks) and evaluated over the time that the subject was involved in the study. RESULTS: Initially, the patient was suffering from severe panic attacks accompanied by vegetative symptoms and from destructive and negative thoughts. After 8 consecutive weeks of treatment encompassing sixteen QEEG neurofeedback training sessions (each lasting 30 min), a subjective improvement of his complaints was reported. More importantly, QEEG records of the patient also improved, approximating the pattern of QEEG recorded in the healthy control. CONCLUSION: In this single case-based feasibility analysis, we demonstrate that systematic application of QEEG-Neurofeedback may result in manifest and durable therapeutic effect. Of note, use of this treatment may be a valuable option for patients with panic attack/panic disorder, especially if related to the psychological burden of the COVID-19/war era. Future studies on a larger patient population, especially with a longitudinal/prospective design, are warranted.

Quantitative electroencephalography interpretation of human brain activity after COVID-19 before and after Sudarshan Kriya Yoga.

The COVID-19 pandemic has affected the entire world. The SARS-CoV-2 virus is wreaking havoc globally, leading to serious health problems and even death. The purpose of this study is to present the brainwave variability pattern using QEEG after exposure to COVID-19 and to introduce the subject of the Sudarshan Kriya Yoga (SKY)-based breathing technique. QEEG is one of the basic neurological examinations through which we can compare the changes in the nervous system after SARS-CoV-2 virus infection and observe the variation of brainwave frequencies with a breathing technique.

A Comparative Cross-Platform Analysis to Identify Potential Biomarker Genes for Evaluation of Teratozoospermia and Azoospermia.

Male infertility is a global public health concern. Teratozoospermia is a qualitative anomaly of spermatozoa morphology, contributing significantly to male infertility, whereas azoospermia is the complete absence of spermatozoa in the ejaculate. Thus, there is a serious need for unveiling the common origin and/or connection between both of these diseases, if any. This study aims to identify common potential biomarker genes of these two diseases via an in silico approach using a meta-analysis of microarray data. In this study, a differential expression analysis of genes was performed on four publicly available RNA microarray datasets, two each from teratozoospermia (GSE6872 and GSE6967) and azoospermia (GSE145467 and GSE25518). From the analysis, 118 DEGs were found to be common to teratozoospermia and azoospermia, and, interestingly, sperm autoantigenic protein 17 (SPA17) was found to possess the highest fold change value among all the DEGs (9.471), while coiled-coil domain-containing 90B (CCDC90B) and coiled-coil domain-containing 91 (CCDC91) genes were found to be common among three of analyses, i.e., Network Analyst, ExAtlas, and GEO2R. This observation indicates that SPA17, CCDC90B, and CCDC91 genes might have significant roles to play as potential biomarkers for teratozoospermia and azoospermia. Thus, our study opens a new window of research in this area and can provide an important theoretical basis for the diagnosis and treatment of both these diseases.

Development of a brain wave model based on the quantitative analysis of EEG and EEG biofeedback therapy in patients with panic attacks during the COVID-19 pandemic.

The current global crisis facing the world is the COVID-19 pandemic. Infection from the SARS-CoV-2 virus leads to serious health complications and even death. As it turns out, COVID-19 not only physically assails the health of those infected, but also leads to serious mental illness regardless of the presence of the disease. Social isolation, fear, concern for oneself and one's loved ones, all of this occurs when a pandemic overloads people. People exhibit numerous neurological disorders that have never happened to them before. Patients are diagnosed with frequent panic attacks, the result of which can be seen in their Quantitative Electroencephalogram results. This test may be one of the main diagnostic tools of the COVID-19 pandemic. From the results obtained, it is possible to compare and draw conclusions. This method of testing effectively allows EEG biofeedback training and observes its effect on brain activity. The feedback received in this way gives us the opportunity to properly tailor a protocol for the patient and their conditions. Numerous studies support the effectiveness of EEG biofeedback for panic attacks and other psychiatric disorders. The purpose of our study was to show the effectiveness of EEG biofeedback with a Quantitative Electroencephalogram of the brainwave pattern after having COVID-19 and what symptoms may result.

Comparison of QEEG Findings before and after Onset of Post-COVID-19 Brain Fog Symptoms.

Previous research and clinical reports have shown that some individuals after COVID-19 infection may demonstrate symptoms of so-called brain fog, manifested by cognitive impairment and disorganization in behavior. Meanwhile, in several other conditions, related to intellectual function, a specific pattern of changes in electric brain activity, as recorded by quantitative electroencephalography (QEEG) has been documented. We hypothesized, that in post-COVID brain fog, the subjective complaints may be accompanied by objective changes in the QEEG profile. In order to test this hypothesis, we have performed an exploratory study on the academic staff of our University with previous records of QEEG originating in the pre-COVID-19 era. Among them, 20 subjects who revealed neurological problems in the cognitive sphere (confirmed as covid fog/brain fog by a clinical specialist) after COVID-19 infection were identified. In those individuals, QEEG was performed. We observed, that opposite to baseline QEEG records, increased Theta and Alpha activity, as well as more intensive sensimotor rhythm (SMR) in C4 (right hemisphere) in relation to C3 (left hemisphere). Moreover, a visible increase in Beta 2 in relation to SMR in both hemispheres could be documented. Summarizing, we could demonstrate a clear change in QEEG activity patterns in individuals previously not affected by COVID-19 and now suffering from post-COVID-19 brain fog. These preliminary results warrant further interest in delineating their background. Here, both neuroinflammation and psychological stress, related to Sars-CoV2-infection may be considered. Based on our observation, the relevance of QEEG examination as a supportive tool for post-COVID clinical workup and for monitoring the treatment effects is also to be explored.

Assessment of physical activity and quality of life of students in the day of COVID-19 pandemic.

INTRODUCTION: Physical activity is one of the intrinsic elements of human life. Systematic exercise is an important factor in preventing heart disease, stroke and type 2 diabetes. Lack of exercise increases the risk of non-communicable disease (NCD) and death worldwide. The outbreak of the COVID-19 pandemic aggravated the problem of the lack of adequate amounts of physical exertion, especially among students. With the introduction of distance learning, they were forced to change their lifestyle, and the physical activity that they had undertaken so far has, in most cases, been reduced or stopped altogether. AIM: In our study, we aimed to find out how students evaluate the quality of life and what actions they take for health during the COVID-19 pandemic. MATERIAL AND METHODS: The study was conducted using the Google online form on student groups and websites using the proprietary survey and the SF-36 quality of life questionnaire. The study group consisted of 162 people (107 women and 55 men). The respondents ranged in age from 19 to 25 years old. RESULTS: The study showed that as many as 77.8% of students undertook various forms of physical activity before the outbreak of the COVID-19 pandemic. Unfortunately, the dangers and restrictions related to the coronavirus, as well as the decrease in mood resulting from this situation, forced nearly half of the students participating in the study to change their forms of physical activity. In most cases, restrictions on access to various forms of recreation discouraged the respondents from engaging in it. As many as 70.4% of the respondents stated that they had experienced health and emotional problems. In most cases, it influenced the performance of daily tasks, contacts with family, friends, neighbors or other social groups. CONCLUSIONS: The COVID-19 pandemic has forced a significant number of students to modify their lifestyle, which has resulted in the reduction or abandonment of physical exercise. The research confirmed the relationship between physical activity and the sense of quality of life.

Effects of SARS-CoV-2 Inflammation on Selected Organ Systems of the Human Body.

INTRODUCTION AND PURPOSE OF THE STUDY: SARS-CoV-2 virus does not only affect the respiratory system. It may cause damage to many organ systems with long-term effects. The latest scientific reports inform that this virus leaves a long-term trace in the nervous, circulatory, respiratory, urinary and reproductive systems. It manifests itself in disturbances in the functioning of the organs of these systems, causing serious health problems. The aim of the study was to review the latest research into the long-term effects of COVID-19 and determine how common these symptoms are and who is most at risk. Based on a literature review using the electronic scientific databases of PubMed and Web of Science on the long-term effects of SARS-CoV-2 infection, 88 studies were included in the analysis. The information contained in the analyzed literature shows that the SARS-CoV-2 virus can cause multi-organ damage, causing a number of long-term negative health complications. CONCLUSIONS: There is evidence that the virus can cause long-term complications lasting more than six months. They mainly concern disturbances in the functioning of the nervous, circulatory and respiratory systems. However, these studies are small or short-lasting, and many are speculative.

Reply to Cafiero et al. Comment on "Kopanska et al. Disorders of the Cholinergic System in COVID-19 Era-A Review of the Latest Research. Int. J. Mol. Sci. 2022, 23, 672".

We have carefully read the Letter to the Editor by Concetta Cafiero, Alessandra Micera, Agnese Re, Beniamino Schiavone, Giulio Benincasa, and Raffaele Palmirotta related to our paper entitled "Disorders of the Cholinergic System in COVID-19 Era-A Review of the Latest Research" [...].

Acrylamide Neurotoxicity as a Possible Factor Responsible for Inflammation in the Cholinergic Nervous System.

Acrylamide (ACR) is a chemical compound that exhibits neurotoxic and genotoxic effects. It causes neurological symptoms such as tremors, general weakness, numbness, tingling in the limbs or ataxia. Numerous scientific studies show the effect of ACR on nerve endings and its close connection with the cholinergic system. The cholinergic system is part of the autonomic nervous system that regulates higher cortical functions related to memory, learning, concentration and attention. Within the cholinergic system, there are cholinergic neurons, anatomical cholinergic structures, the neurotransmitter acetylcholine (ACh) and cholinergic receptors. Some scientific reports suggest a negative effect of ACR on the cholinergic system and inflammatory reactions within the body. The aim of the study was to review the current state of knowledge on the influence of acrylamide on the cholinergic system and to evaluate its possible effect on inflammatory processes. The cholinergic anti-inflammatory pathway (CAP) is a neuroimmunomodulatory pathway that is located in the blood and mucous membranes. The role of CAP is to stop the inflammatory response in the appropriate moment. It prevents the synthesis and the release of pro-inflammatory cytokines and ultimately regulates the local and systemic immune response. The cellular molecular mechanism for inhibiting cytokine synthesis is attributed to acetylcholine (ACh), the major vagal neurotransmitter, and the α7 nicotinic receptor (α7nAChR) subunit is a key receptor for the cholinergic anti-inflammatory pathway. The combination of ACh with α7nAChR results in inhibition of the synthesis and release of pro-inflammatory cytokines. The blood AChE is able to terminate the stimulation of the cholinergic anti-inflammatory pathway due to splitting ACh. Accordingly, cytokine production is essential for pathogen protection and tissue repair, but over-release of cytokines can lead to systemic inflammation, organ failure, and death. Inflammatory responses are precisely regulated to effectively protect against harmful stimuli. The central nervous system dynamically interacts with the immune system, modulating inflammation through the humoral and nervous pathways. The stress-induced rise in acetylcholine (ACh) level acts to ease the inflammatory response and restore homeostasis. This signaling process ends when ACh is hydrolyzed by acetylcholinesterase (AChE). There are many scientific reports indicating the harmful effects of ACR on AChE. Most of them indicate that ACR reduces the concentration and activity of AChE. Due to the neurotoxic effect of acrylamide, which is related to the disturbance of the secretion of neurotransmitters, and its influence on the disturbance of acetylcholinesterase activity, it can be concluded that it disturbs the normal inflammatory response.