RESUMO
m-Hydroxybenzoylecgonine (m-OH-BE) and d3-m- hydroxybenzoylecgonine (d3-m-OH-BE) have been synthesized, and a GC/MS procedure with d3-m-OH-BE as internal standard has been developed. Among 24 human urine specimens that were positive for BE, all of them have shown detectable levels of m-OH-BE with 75% of the specimens exceeding the LoQ (5 ng/mL), compared with only 50% of the specimens containing detectable levels of EME. The presence of m-OH-BE in urine suggested that this metabolite may serve as a valuable marker of cocaine use in addition to BE and EME.
Assuntos
Transtornos Relacionados ao Uso de Cocaína , Cocaína/análogos & derivados , Medicina Legal , Cromatografia Gasosa-Espectrometria de Massas/métodos , Cocaína/metabolismo , Cocaína/farmacocinética , Cocaína/urina , HumanosRESUMO
The effect of drying intact clippings of Taxus on the recovery of taxol and related compounds was studied under different drying conditions which included tobacco drying barn, greenhouse, shadehouse, air conditioned laboratory, oven, and freeze-drying. For clippings dried under tobacco barn, greenhouse, oven, and freeze-drying conditions, nearly total recovery of the expected levels based upon projections from analysis of fresh biomass was observed for taxol and cephalomannine. However, only 75-80% of the expected values for 10-deacetyltaxol and 10-deacetylbaccatin III were found. When the length of drying was extended up to 10 and 15 days as in the shadehouse and laboratory conditions, the recovery of all taxanes was adversely affected.
