Examination of single-stage anaerobic and anoxic/aerobic and dual-stage anaerobic-anoxic/aerobic digestion to remove pharmaceuticals from municipal biosolids.

Many trace contaminants of emerging concern (CECs) including a number of pharmaceutically active compounds are not effectively removed during conventional wastewater treatment processes and instead accumulate in wastewater sludge. Unfortunately, many existing sludge stabilization treatments such as anaerobic digestion (AD) also have limited effectiveness against many of these CECs including the four pharmaceuticals ibuprofen, diclofenac, carbamazepine, and azithromycin which can then enter the environment through the disposal or land application of biosolids. Single-stage AD, single-stage cycling aerobic-anoxic (AERO/ANOX) and sequential digesters (AD followed by an AERO/ANOX digester) at sludge retention times (SRT) of 5 to 20-days were evaluated side-by-side to assess their effectiveness in removing pharmaceuticals and conventional organic matter. Single-stage ADs (35 °C) and AERO/ANOX (22 °C) digesters effectively removed total solids while sequential AD + AERO/ANOX digesters offered further improvements. Ibuprofen was not effectively removed during AD and resulted in up to a 23 ± 8% accumulation. However, ibuprofen was completely removed during AERO/ANOX digestion and in several sequential digestion scenarios. Each type of digestion was less effective against carbamazepine with slight (3 ± 2%) accumulations to low levels (14 ± 1%) of removals in each type of digestion studied. Diclofenac was more effectively removed with up 30 ± 3% to 39 ± 4% reductions in the single-stage digesters (AD and AERO/ANOX, respectively). While sequential digestion scenarios with the longest aerobic SRTs significantly increased diclofenac removals from their first-stage digesters, scenarios with the longest anaerobic SRTs actually decreased removals from first-stage digesters, possibly due to reversible biotransformation of diclofenac conjugates/metabolites. Up to 43 ± 6% of azithromycin was removed in AERO/ANOX digesters, while the best performing sequential-digester scenario removed up to 63 ± 7% of azithromycin. This study shows that different digester configurations can reduce the CEC burden in biosolids while also greatly reducing their volumes for disposal, although none can remove CECs completely.

A Review on the Fate of Legacy and Alternative Antimicrobials and Their Metabolites during Wastewater and Sludge Treatment.

Antimicrobial compounds are used in a broad range of personal care, consumer and healthcare products and are frequently encountered in modern life. The use of these compounds is being reexamined as their safety, effectiveness and necessity are increasingly being questioned by regulators and consumers alike. Wastewater often contains significant amounts of these chemicals, much of which ends up being released into the environment as existing wastewater and sludge treatment processes are simply not designed to treat many of these contaminants. Furthermore, many biotic and abiotic processes during wastewater treatment can generate significant quantities of potentially toxic and persistent antimicrobial metabolites and byproducts, many of which may be even more concerning than their parent antimicrobials. This review article explores the occurrence and fate of two of the most common legacy antimicrobials, triclosan and triclocarban, their metabolites/byproducts during wastewater and sludge treatment and their potential impacts on the environment. This article also explores the fate and transformation of emerging alternative antimicrobials and addresses some of the growing concerns regarding these compounds. This is becoming increasingly important as consumers and regulators alike shift away from legacy antimicrobials to alternative chemicals which may have similar environmental and human health concerns.

Occurrence of the Persistent Antimicrobial Triclosan in Microwave Pretreated and Anaerobically Digested Municipal Sludges under Various Process Conditions.

Treatment of emerging contaminants, such as antimicrobials, has become a priority topic for environmental protection. As a persistent, toxic, and bioaccumulative antimicrobial, the accumulation of triclosan (TCS) in wastewater sludge is creating a potential risk to human and ecosystem health via the agricultural use of biosolids. The impact of microwave (MW) pretreatment on TCS levels in municipal sludge is unknown. This study, for the first time, evaluated how MW pretreatment (80 and 160 °C) itself and together with anaerobic digestion (AD) under various sludge retention times (SRTs: 20, 12, and 6 days) and temperatures (35 and 55 °C) can affect the levels of TCS in municipal sludge. TCS and its potential transformation products were analyzed with ultra-high-performance liquid chromatography and tandem mass spectrometry. Significantly higher TCS concentrations were detected in sludge sampled from the plant in colder compared to those in warmer temperatures. MW temperature did not have a discernible impact on TCS reduction from undigested sludge. However, AD studies indicated that compared to controls (no pretreatment), MW irradiation could make TCS more amenable to biodegradation (up to 46%), especially at the elevated pretreatment and digester temperatures. At different SRTs studied, TCS levels in the thermophilic digesters were considerably lower than that of in the mesophilic digesters.

Occurrence and fate of antimicrobial triclocarban and its transformation products in municipal sludge during advanced anaerobic digestion using microwave pretreatment.

This study, for the first time, investigated the impact of microwave pretreatment on the fate of the pervasive antimicrobial triclocarban (TCC) that was already present in municipal sludge, before and during advanced anaerobic digestion (AD) under thermophilic and mesophilic conditions. A range of microwave temperature (80 and 160 °C) and exposure duration (1 and 30 min) configurations were studied by employing ten bench-scale anaerobic digesters fed with mixed sludge at three different solids retention times (SRTs) including 20, 12, and 6 days. Seasonal changes influenced the levels of TCC in municipal sludge sampled from a plant employing the biological nutrient removal. Initial batch pretreatment studies showed that microwave irradiation itself can achieve TCC removal efficiencies up to 30 ± 4 and 64 ± 5% at 80 and 160 °C, respectively. The control digesters utilizing un-pretreated mixed sludge showed limited TCC removals, between 18 and 32% and 11-26% respectively, under thermophilic and mesophilic temperatures. On the other hand, the highest TCC elimination (78 ± 2%) was obtained from the thermophilic digester utilizing microwaved sludge at 160 °C for 30 min at SRT of 12 days. The non-chlorinated carbanilide (a transformation product of TCC) was detected and quantified for the first time during conventional and microwave-pretreated anaerobic sludge digestion. The formation of carbanilide in biosolids through reductive dechlorination could be an indicator of efficient and complete TCC transformation. This research demonstrated that AD coupled with microwave pretreatment can be used to reduce environmental concentrations of TCC in municipal sludge and biosolids.

Membrane integrated process for advanced treatment of high strength Opium Alkaloid wastewaters.

In this study, an integrated aerobic membrane bioreactor (MBR)-nanofiltration (NF) system has been applied for advanced treatment of Opium processing wastewaters to comply with strict discharge limits. Aerobic MBR treatment was successfully applied to high strength industrial wastewater. In aerobic MBR treatment, a non-fouling unique slot aeration system was designed using computational fluid dynamics techniques. The MBR was used to separate treated effluent from dispersed and non-settleable biomass. Respirometric modeling using MBR sludge indicated that the biomass exhibited similar kinetic parameters to that of municipal activated sludge systems. Aerobic MBR/NF treatment reduced chemical oxygen demand (COD) from 32,000 down to 2,500 and 130 mg/L, respectively. The MBR system provided complete removal of total inorganic nitrogen; however, nearly 50 mgN/L organic nitrogen remained in the permeate. Post NF treatment after MBR permeate reduced nitrogen below 20 mgN/L, providing nearly total color removal. In addition, a 90% removal in the conductivity parameter was reached with an integrated MBR/NF system. Finally, post NF application to MBR permeate was found not to be practical at higher pH due to low flux (3-4 L/m2/hour) with low recovery rates (30-40%). As the permeate pH lowered to 5.5, 75% of NF recovery was achieved at a flux of 15 L/m2/hour.

Is the chronic impact of sulfamethoxazole different for slow growing culture? The effect of culture history.

The study evaluated impact of sulfamethoxazole on acetate utilization kinetics and microbial community structure using respirometric analysis and pyrosequencing. A fill and draw reactor fed with acetate was sustained at a sludge age of 10 days. Acute impact was assessed by modeling of respirometric data in batch reactors started with sulfamethoxazole doses in the range of 25-200 mg/L. Fill and draw operation resumed with continuous sulfamethoxazole dosing of 50 mg/L and the chronic impact was evaluated with acclimated biomass after 20 days. Acute impact revealed higher maintenance energy requirements, activity reduction and slight substrate binding. Chronic impact resulted in retardation of substrate storage. A fraction of acetate was utilized at a much lower rate with partial biodegradation of sulfamethoxazole by the acclimated biomass. Pyrosequencing indicated that Amaricoccus sp. and an unclassified Bacteroidetes sp., possibly with the ability to co-metabolize sulfamethoxazole, dominated the community.

Effect of sludge age on population dynamics and acetate utilization kinetics under aerobic conditions.

The study addressed acetate utilization by an acclimated mixed microbial culture under different growth conditions. It explored changes in the composition of the microbial community and variable process kinetics induced by different culture history. Sequencing batch reactors were operated at steady-state at different sludge ages of two and ten days. Microbial population structure was determined using high-throughput sequencing of 16S rRNA genes. Parallel batch experiments were conducted with acclimated biomass for respirometric analyses. A lower sludge age sustained a different community, which also reflected as variable kinetics for microbial growth and biopolymer storage. The maximum growth rate was observed to change from 3.9/d to 8.5/d and the substrate storage rate from 3.5/d to 5.9/d when the sludge age was decreased from 10 d to 2.0 d. Results challenge the basic definition of heterotrophic biomass in activated sludge models, at least by means of variable kinetics under different growth conditions.