Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
2.
PLoS One ; 12(1): e0170015, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28081235

RESUMO

Mycoplasma agalactiae is a worldwide serious pathogen of small ruminants that usually spreads through the mammary route causing acute to subacute mastitis progressing to chronic persistent disease that is hard to eradicate. Knowledge of mechanisms of its pathogenesis and persistence in the mammary gland are still insufficient, especially the host-pathogen interplay that enables it to reside in a chronic subclinical state. This study reports transcriptome profiling of mammary tissue from udders of sheep experimentally infected with M. agalactiae type strain PG2 in comparison with uninfected control animals using Illumina RNA-sequencing (RNA-Seq). Several differentially expressed genes (DEGs) were observed in the infected udders and RT-qPCR analyses of selected DEGs showed their expression profiles to be in agreement with results from RNA-Seq. Gene Ontology (GO) analysis revealed majority of the DEGs to be associated with mycoplasma defense responses that are directly or indirectly involved in host innate and adaptive immune responses. Similar RNA-Seq analyses were also performed with spleen cells of the same sheep to know the specific systemic transcriptome responses. Spleen cells exhibited a comparatively lower number of DEGs suggesting a less prominent host response in this organ. To our knowledge this is the first study that describes host transcriptomics of M. agalactiae infection and the related immune-inflammatory responses. The data provides useful information to further dissect the molecular genetic mechanisms underlying mycoplasma mastitis, which is a prerequisite for designing effective intervention strategies.


Assuntos
Glândulas Mamárias Animais/metabolismo , Infecções por Mycoplasma/fisiopatologia , Mycoplasma agalactiae/fisiologia , RNA/metabolismo , Ovinos/genética , Transcriptoma , Animais , Modelos Animais de Doenças , Feminino , Perfilação da Expressão Gênica , Imunoensaio , Imuno-Histoquímica , Glândulas Mamárias Animais/microbiologia , Glândulas Mamárias Animais/patologia , Infecções por Mycoplasma/metabolismo , Infecções por Mycoplasma/microbiologia , Mycoplasma agalactiae/imunologia , RNA/química , RNA/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real , Análise de Sequência de RNA
3.
Virol J ; 12: 146, 2015 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-26377918

RESUMO

BACKGROUND: Gammaherpesviruses (GHVs) are a large group of dsDNA viruses that can infect humans and several animal species. The two human GHVs, Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus are known for their oncogenic properties in individuals with immunodeficiency. Recently, the first feline GHV, Felis catus gammaherpesvirus 1 (FcaGHV1) was discovered and frequently found in domestic cats in Australia, Singapore and the USA. FcaGHV1 is more likely to be detected in cats co-infected with the feline immunodeficiency virus (FIV). FINDINGS: The prevalence of FcaGHV1 in pet cats from Germany and Austria was 16.2 % (95 % CI = 12.38-20.02). The odds for GHV infection were greater for FIV positive (OR = 4.5), male (OR = 13.32) and older (OR = 2.36) cats. Furthermore, FcaGHV1 viral loads were significantly higher in FIV-infected cats compared to matched controls. CONCLUSIONS: GHV infections are common in domestic cats in Central Europe. The worldwide distribution of FcaGHV1 can be assumed. A potential role as a co-factor in FIV-induced pathogeneses is supported.


Assuntos
Doenças do Gato/epidemiologia , Gammaherpesvirinae/isolamento & purificação , Infecções por Herpesviridae/veterinária , Animais , Áustria/epidemiologia , Doenças do Gato/virologia , Gatos , Síndrome de Imunodeficiência Adquirida Felina/complicações , Feminino , Alemanha/epidemiologia , Infecções por Herpesviridae/epidemiologia , Masculino , Dados de Sequência Molecular , Prevalência , Fatores de Risco , Análise de Sequência de DNA , Carga Viral
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...