RESUMO
Chemotactic effects of staphylococcal substances on macrophages from guinea pigs were studied using modified Boyden-chambers. Filters with an average pore-diameter of 12 micrometer allowed optimal migration of the macrophages. The macrophages were obtained from the peritoneal exsudates 96 h after stimulation with sodium-caseinate (fig. 1) and subsequently concentrated by centrifugation in a ficoll-ronpacon gradient (fig. 2, table 1). Casein had strong chemotactic effects on the macrophages with and without fresh guinea pig serum (fig. 3). Staphylococci, before and after extraction with guanidinium chloride, purified protein A and capsular substances were neither cytotaxic nor cytotaxigenic (in the absence resp. presence of fresh serum). Culture supernatants and staphylococci after incubation with their homologous antiserum proved cytotaxigenic, also lysates of granulocytes from the blood. Lysates of granulocytes from the peritoneal exsudates of guinea pigs were strongly cytotaxigenic for the macrophages.
