[An analysis of the mechanisms of interpopulation interaction of Yersinia with Tetrahymena pyriformis infusorians at the cellular and subcellular levels]. / Analiz mekhanizmov mezhpopuliatsionnogo vzaimodeistviia iersinii s infuzoriiami Tetrahymena pyriformis na kletochnom i subkletochnom urovniakh.

The mechanisms of interaction between the populations of Yersinia and T. pyriformis have been analyzed on the cellular and subcellular levels. As shown in this investigation, Yersinia, when phagocytized by T. pyriformis, may undergo morphological changes, remain unchanged and also multiply, destroying the host cell in the process.

[Leukocytic thermostable alpha-glycoprotein and lactoferrin from granulocyte cytoplasm (immunocytochemical study)]. / Leikotsitarynyi termostabil'nyi alpha-glikoproteid i laktoferrin tsitoplazmy granulotsitov (immunotsitokhimicheskoe issledovanie).

The immunofluorescence techniques with monospecific antibodies to lactoferrin (LF) and leukocytic thermostable alpha-glycoprotein (LT alpha-G) has demonstrated that both the proteins are specific components of the peripheral blood granulocytes of normal subjects. LF and LT alpha-G are detectable in the cytoplasmic granules of polynuclear leukocytes but not on the external surface of these cells' membranes. Differences between the direct and indirect immunofluorescence techniques, used for the detection of the granulocytic LF and LT alpha-G are discussed. Examinations of the blood smears from patients, operated on under artificial circulation, have shown that the emergence of granulocytic protein aggregates between the cells and LF and LT alpha-G sorption on the red cell surface indicate a disease.

[Use of a calibrated melamine-formaldehyde latex for the luminescence microscopic study of phagocytosis in a macrophage culture]. / Primenenie kalibrovannogo melamin-formal'degidnogo lateksa dlia liuminestsentno-mikroskopicheskogo izucheniia fagotsitoza v kul'ture makrofagov.

The possibility has been demonstrated of the use of calibrated melamine-formaldehyde latex for studying phagocytosis as an object not undergoing intracellular digestion. Latex was discovered to be actively phagocytized by macrophages and to exert no toxic action on them. A study was made of the time course of changes in the color and brightness of the fluorescence of the latex phagocytized particles in macrophages intravitally fluorochrominated by acridine orange. These changes were demonstrated to be analogous to those observed previously during phagocytosis of bacteria and other objects. The data obtained demonstrate once more the transition of the fluorescent complex from lysosomes to phagosomes and the lack of the relationship of these changes with intracellular death and digestion of the phagocytized objects. The possibility has been also shown to differentiate between phagocytized particles of latex and those located outside the cells.

[Study of the kinetics of antigen-antibody reaction by light scattering]. / Nabliudenie kinetiki reaktsii antigen-antitelo metodom svetorasseianiia.

Laser light scattering in conjunction with measurement of the spectral width and integral intensity of light scattering was applied to studying the process of complex formation of antigens and antibodies. The system of polysaccharide group A streptococcus and antibody against it was examined under varying polysaccharide concentrations. The measurements were performed every 10 s for 70 min after combining polysaccharide and antibody solutions. Within the entire time interval, the size of the complexes were less than the wave length of exciting laser light (0.633 m). This made it possible to determine their average magnitude in terms of the Rayleigh model.

[Detection of antigen-antibody reactions using optical beat spectroscopy]. / Obnaruzhenie reaktsii antigen-antitelo s pomoshch'iu spektroskopii opticheskogo smesheniia.

The light beating spectroscopy technique was used for recording the results of serological reactions without visual phenomena. The investigated systems consisted of the antigens of influenza virus or Mycoplasma pneumoniae and the corresponding sera. A principal possibility of detecting the antigen-antibody complexes in serological reactions without visual phenomena by the light beating spectroscopy technique was demonstrated.

[Use of the light-dispersion spectroscopy for detection of the precipitation reaction]. / Primenenie metoda spektroskopii opticheskogo smeshcheniia dlia obnaruzheniia reaktsii pretsipitatsii.

The light beating spectroscopy technique was used to detect the precipitation test. The system consisting of human serum globulin--rabbit antiserum against human globulins was studied. A possibility of detecting the precipitation test for much more diluted systems of the antigen and antibody by means of the light beating technique than by the routine visual method was demonstrated.

[Characteristics of the reaction of the macrophage lysosomal apparatus in guinea pigs immunized with a live and a killed Br. abortus culture]. / Osobennosti reaktsii lizosomal'nogo apparata makrofagov morskikh svinok, immunizirovannykh zhivoi i ubitoi kul'turoi Br. abortus

Accumulation of fluorescent antigen-antibody complex in the cytoplasmic granules at various time intervals after the phagocytosis of brucellae treated with brucella fluorescent serum was studied in the peritoneal macrophage culture of guinea pigs immunized with live and killed brucella culture. The activity of the lysosomic system was also studied. Immunization with live brucellae led to an increase in the acid phosphatase activity, to an increased rate of destruction of phagocytosed spheroplasts with fluorescent antibodies fixed on their surface, to a more rapid accumulation of fluorescent antigen-antibody complex in secondary lysosomes and to a more marked humoral response than the immunization with the killed culture. Changes in macrophages correlated with positive skin allergic reactions, and were apparently associated with increased sensitivity of a delayed type.

[Effect of antilysosomal sera on the phagocytosis of corpuscular antigens in a macrophage culture]. / Vliianie antilizosomnykh syvorotok na fagotsitoz korpuskuliarnykh antigenov v kul'ture makrofagov

A study was made of the effect of the antilysosomal sera on various phases of phagocytosis Macrophages fluorochromized with acridine orange served as a model for the assessment of the effect of the antilysosomal sera on the phagocytosis: the fluorescent-microscopic method permitted to evaluate quantitatively the activity of the antilysosomal sera and to study the intracellular changes in the phagocytized antigen associated with its interaction with the lysosomes. The data obtained showed the antisera to the enzymes and the lysosome membranes to inhibit the phagocytosis both in the presence of a complement and without it. It was also demonstrated that the antilysosomal sera influenced the activity of the acid phosphatase and its distribution in the macrophages.

[Penetration of anti-lysosome serum in macrophages and the localization of anti-lysosome antibodies in their structures]. / Proniknovenie antilizosomnykh syvorotok v makrofagi i lokalizatsiia antilizosomnykh antitel na ikh struktyrakh

Penetration of antilysosomal sera into living macrophages, their localization on subcellular structures, and their effects upon the cell, were studied by means of immunofluorescence technique. The rabbit antilysosomal (antimembrane) immune globulins were found to be ingested by the mouse macrophages and to exert obvious effect upon the macrophage function. The specificity of used antisera was confirmed by findings obtained with fixed macrophages.