RESUMO
AIM: Determine the role of opportunistic infections causative agents in ethology of obstructive bronchitises and prolonged subfebrilities in children. MATERIALS AND METHODS: 56 children with the diagnosis of obstructive bronchitis and 46 children with the diagnosis of prolonged subfebrility were examined for the presence of herpes, mycoplasma and pneumocystic infections. EIA, IIF, rapid culture method, PCR were used. RESULTS: The highest number of cases of mixed infection was detected in children with HHV-6 infection. Mixed infection was diagnosed 6 times more frequently in children with obstructive bronchitis and 9 times in children with prolonged subfebrility. The number of children with pneumocystosis in combination with other infections was 2.4 and 2 times higher than with monoinfection; with CMV infection--4 and 2 times; with HSV infection--5 and 4 times; EBV infection--6 and 3.7 times. The only exception was mycoplasmosis detected in children with obstructive bronchitis where the difference between the number of mono and mixed infection cases was insignificant. CONCLUSION: The data obtained give evidence of wide spread of opportunistic infections.
Assuntos
Bronquite/diagnóstico , Coinfecção/diagnóstico , Febre/diagnóstico , Infecções Oportunistas/diagnóstico , Biomarcadores/sangue , Bronquite/sangue , Bronquite/etiologia , Criança , Doença Crônica , Coinfecção/sangue , Coinfecção/complicações , Infecções por Citomegalovirus/sangue , Infecções por Citomegalovirus/complicações , Infecções por Citomegalovirus/diagnóstico , Ensaio de Imunoadsorção Enzimática , Infecções por Vírus Epstein-Barr/sangue , Infecções por Vírus Epstein-Barr/complicações , Infecções por Vírus Epstein-Barr/diagnóstico , Febre/sangue , Febre/etiologia , Herpes Simples/sangue , Herpes Simples/complicações , Herpes Simples/diagnóstico , Herpesvirus Humano 6/genética , Herpesvirus Humano 6/isolamento & purificação , Humanos , Imunoglobulinas/sangue , Infecções por Mycoplasma/sangue , Infecções por Mycoplasma/complicações , Infecções por Mycoplasma/diagnóstico , Infecções Oportunistas/sangue , Infecções Oportunistas/complicações , Pneumonia por Pneumocystis/sangue , Pneumonia por Pneumocystis/complicações , Pneumonia por Pneumocystis/diagnóstico , Reação em Cadeia da Polimerase , Infecções por Roseolovirus/sangue , Infecções por Roseolovirus/complicações , Infecções por Roseolovirus/diagnósticoRESUMO
Contemporary aspects ofthe problem ofdiseases caused by single-celled yeast fungi Pneumocystisjirovecii are examined. Pneumocystic infection affects children and adults with various manifestations of immunodeficiency, is a HIV-associated infection. Contemporary epidemiologic characteristics of this infection are presented, data on etiology, pathogenesis and clinical presentation ofpneumocystosis are provided, problems of laboratory diagnostics are elucidated. Morphologic, immunobiologic and molecular-genetic methods of detection of Pneumocystis jirovecii causative agent are described.
Assuntos
Infecções por HIV/imunologia , Hospedeiro Imunocomprometido , Pneumocystis carinii/imunologia , Pneumonia por Pneumocystis/imunologia , Adulto , Criança , Imunofluorescência , Infecções por HIV/complicações , Infecções por HIV/diagnóstico , Infecções por HIV/virologia , Humanos , Pneumocystis carinii/genética , Pneumocystis carinii/isolamento & purificação , Pneumonia por Pneumocystis/complicações , Pneumonia por Pneumocystis/diagnóstico , Pneumonia por Pneumocystis/microbiologia , Reação em Cadeia da PolimeraseRESUMO
Transmission electron microscopy (of ultrathin sections) was used to examine the biomass of lung tissue in the immunodeficiency minipigs experimentally infected with Pneumocystis carinii. The material was found to contain pneumocysts, bacteria, and coronaviruses. There was a clear coronavirus-pneumocyst structural relationship. The findings suggest the combined effect of microorganisms of different systematic groups on the development of a pathological process in the experimental infection etiologically determined by Pneumocystis carinii.
