[Enzymatic determination of serum glycerophosphatides, using phospholipase C (author's transl)]. / Enzymatische Bestimmung der Glycerinphosphatide im Serum unter Verwendung von Phospholipase C.

A simple method is described for the determination of serum glycerophosphatides, with the exception of lyso-phosphatide. Two determinations are necessary. The usual "triglyceride" determination is performed on native serum, and a further determination is performed after incubation of the serum with phospholipase C. Glycerophosphatides are hydrolysed to diglycerides and phosphorylcholine by phospholipase C. The resulting diglycerides are determined in addition to the mono-, di-, triglycerides and free glycerol of the native serum. The difference between the two glyceride determinations corresponds to the concentration of glycerophosphatides. Involvement of glycerophosphatides in blood coagulation and their increased levels in certain liver illnesses are two reasons for the importance of the present method. Compared with the usual method of measuring phospholipid phosphorus, the present method involves considerably less work, and, as a fully enzymatic method, it has a higher specificity.

[A reagent-saving modification of the glucose dehydrogenase method for the determination of glucose in venous and capillary blood using the Autoanalyzer II (author's transl)]. / Reagenziensparende Modifikation der Glucose-Dehydrogenase-Methode für den AutoAnalyzer II zur Glucosebestimmung in Venen- und Kapillarblut.

A modification of the glucose dehydrogenase method for the Autoanalyzer II is described, which permits a considerable reduction in the quantities of enzymes and coenzyme, without affecting linearity. Using the new modification and a sample frequency of 80 per hour, glucose can be determined in venous blood and in diluted capillary blood haemolysates with no change in the construction of the apparatus.