RESUMO
The effects of transformation of downy birch (Betula pubescens Ehrh.) with the GS1 gene encoding the cytosolic form of glutamine synthetase on the rooting of plants in vitro was studied. The transgenic plants had an elevated content of glutamine as well as glutamic and aspartic acids and rooted more rapidly than the control plants. Rooting on a medium containing the glutamine synthetase inhibitor phosphinothricin prevented the accumulation of auxin in birch plants carrying the GS1 gene, indicating the involvement of this enzyme in raising the level of auxins in the transgenic plants. The correlation between the increase in the auxin levels in the transgenic plants carrying the glutamine synthetase gene and the increase in the rooting rate is shown for the first time.
Assuntos
Betula , Glutamato-Amônia Ligase , Ácidos Indolacéticos/metabolismo , Proteínas de Plantas , Raízes de Plantas , Plantas Geneticamente Modificadas , Betula/enzimologia , Betula/genética , Regulação Enzimológica da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Glutamato-Amônia Ligase/biossíntese , Glutamato-Amônia Ligase/genética , Proteínas de Plantas/biossíntese , Proteínas de Plantas/genética , Raízes de Plantas/enzimologia , Raízes de Plantas/genética , Plantas Geneticamente Modificadas/enzimologia , Plantas Geneticamente Modificadas/genéticaRESUMO
The article presents the results of investigation of violations in the proteolysis system in patients with type 2 diabetes and diabetic retinopathy (DR). We studied the levels of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinase-I (TIMP-1) in the blood and in the intraocularfluid ofpatients with type 2 diabetes depending upon the stage of DR. We have established that the level of MMP-9 and TIMP- in the blood and in the intraocular fluid increased in patients with type 2 diabetes without signs of DR and with any stage of DR compared to the levels of these parameters in patients without diabetes. Increasing the levels of MMP-9 and TIMP-J took place with increasing of DR severity and reached its maximum levels at the proliferative stage of DR. It was found that the levels of MMP-9 in the intraocular fluid and type 2 diabetes duration affected theprobability of developing diabetic macular edema. At the level of MMP-9 105 ng/ml and 2 diabetes duration 10 years, the probability of developing macular edema was 100%. As a result of the construction ofpredictive models it was found that the level of MMP-9 in the intraocular fluid, stage of DR at the beginning of observation and type 2 diabetes duration had influence on the probability of the development ofproliferative DR over 4 years offollow-up. If the level of MMP-9 100 ng/ml, type 2 diabetes duration 10 years and absence of DR at the beginning of observation were observed the probability of developing proliferative DR would compose 85.9%.
