[Isolation and physico-chemical characteristics of ceruloplasmin obtained from murine serum]. / Vydelenie i fiziko-khimicheskaia kharakteristika tseruloplazmina, poluchennogo iz syvorotki krovi myshei.

Ceruloplasmin was isolated from mice blood serum by means of ion exchange chromatography and gel filtration. Its content in blood was equal to 10-12 mg/100 ml, oxidase activity--9-12 U/mg of protein at E440, molecular mass--161.3 +/- 5.8 kDa. Properties of mice ceruloplasmin were studied as compared with the human and rat glycoprotein, isolated using the similar procedure.

[The effect of ceruloplasmin on hepatocyte proliferation in the regenerating rat liver]. / Vliianie tseruloplazmina na proliferatsiiu gepatotsitov regeneriruiushchei pecheni krys.

The influence of ceruloplasmin on cell proliferation in regeneration liver of the rat has been studied. Ceruloplasmin stimulates cell proliferation in regeneration liver increasing functional activity of the mononuclear phagocytes.

[Changes in cell membrane proteins during N-nitrosodiethylamine induced carcinogenesis]. / Izmeneniia belkov kletochnykh membran v protsesse kantserogeneza, vyzvannogo N-nitrozodiétilaminom.

The composition of rat liver cell membrane proteins during the N-nitrosodiethylamine-induced hepatocarcinogenesis was studied using polyacrylamide gel electrophoresis. The effect of the carcinogen on rat liver was controlled by the catalase activity in the microsomal fractions. The plasma membrane protein fractions with Mr of 140 kDa, 135 kDa, 40 kDa and 22 kDa as well as the 36 kDa fraction from endoplasmic reticulum membranes were found to decrease during hepatocarcinogenesis.

[The effect of ceruloplasmin on metabolic processes in the liver of mice during neutron exposure]. / Vliianie tseruloplazmina na metabolicheskie protsessy v pecheni myshei pri neitronnom vozdeistvii.

A single intraperitoneal administration of human ceruloplasmin to BALB/c mice 60 min before fast neutron irradiation normalizes lipid content and cAMP/cGMP index, and increases catalase activity in the liver of irradiated animals.

[Characteristics of the fractional composition of membranes of membrane-bound ribosomes]. / Osobennosti fraktsionnogo sostava belkov membranosviazannykh ribosom.

Electrophoresis in PAAG was used to study the fractional composition of proteins from free and membrane-bound ribosomes isolated from the rat liver. The membrane-bound ribosomes are shown to differ in the protein composition from the free ones in the presence of two fractions with a molecular weight of 35 and 50 kDa which are localized in a large subunit of the organelle. The possible role of these fractions in ribosome-membrane interaction is discussed.

[Protein of membrane-bound liver ribosomes changing after exposure of an organism to various factors]. / Belok membranosviazannykh ribosom pecheni zhivotnykh, izmeniaiushchiisia pri razlichnykh vozdeistviiakh na organizm.

Polyacrylamide gel electrophoresis was used to examine the protein composition of membrane-bound ribosomes from mouse liver upon exposure to different influences, e.g., cell transplantation, cold stress, injections of heterogeneous proteins and cytotoxic non-protein substances, etc. Significant changes in the protein (Mr 50 000) structure occurring immediately after the exposure and depending on its nature were revealed.

[Effect of ceruloplasmin on the growth of experimental tumors and proliferative activity of cells]. / Vliianie tseruloplazmina na rost éksperimental'nykh opukholei i proliferativnuiu aktivnost' kletok.

Exogenous ceruloplasmin influence on experimental tumour growth and intensity of normal and malignant cell proliferation in tissue culture was investigated. It was found that ceruloplasmin administration to animals with transplanted tumours caused a 25-50% inhibition of tumour growth. A growth inhibiting activity of ceruloplasmin observed in tissue culture at the concentration of 5 mg/ml was established. It is also shown that ceruloplasmin did not reduce the antitumour effect of cyclophosphamide.