[Possibility of determining the urinary content of albumin by capillary electrophoresis].

An assay has been developed to determine urinary albumin by capillary electrophoresis. The detection limit for albumin is 6.0 mg/l; its correlation coefficient is 5.3%; the calibration dependence is linear in the range of albumin concentrations of up to 150 mg/l. The findings are in good agreement with the results of immunoturbidimetry (r = 0.996). In addition of albumin, the proposed procedure can detect the presence of other proteins in urine.

[Blood amino acid composition of poikilothermic vertebrate under prolonged starvation]. / Aminokislotnyi pul krovi dlitel'no golodaiushchikh poikilotermnykh pozvonochnykh.

Free amino acids in the blood plasma of lamprey (Lampetra fluviatilis) and frog (Rana temporaria) have been determined quantitatively for the periods of deprivation of the exogenous feed. The lamprey's total amino acid pool increased by 74% from November to April and reached the lower limit known for the mammals. The amount of free amino acids in frogs decreased by 40% in the spring as compared with the autumn values. The difference is accounted for by certain features of the living cycles of these animals. A more energetic proteolysis in the lamprey tissues as compared with that in the frog tissues has been confirmed by quantitative determining of leucine, isoleucine and valine in the blood of these animals. Apart from the above, alanine, glycine, lysine, threonine and, in certain periods, tyrosine have been found to be quantitatively significant in the plasma of both animal species. The composition and proportion of the amino acids in blood plasma of these animals are due to specific features of their metabolism and connected with the energy state of the liver cells under starvation.

[Epidural anesthesia using local anesthetics and clopheline in geriatric urology]. / Epidural'naia anesteziia s ispol'zovaniem mestnykh anestetikov i klofelina v geriatricheskoi urologii.

Study of pharmacokinetics of lidocaine, trimecaine, and clofelin demonstrated rapid resorption of these drugs in the postoperative period. Comparison of two methods of epidural anesthesia in high-risk patients (ASA 3) subjected to urologic surgery showed the objective advantages of adrenergic regional anesthesia, ensuring the stability of vital functions during surgery and allowing operations in awaken patients with adequate spontaneous respiration.

[Free amino acids in the blood plasma of lampreys, frogs and rats]. / Svobodnye aminokisloty v plazme krovi minog, liagushek i krys.

Free amino acids in the blood plasma of lampreys, frogs and rats were determined by HPLC. In spite of quantitative differences in the total pool of free amino acids, the specific content of physiologically important amino acids is quite similar in representatives of different classes of vertebrates, from cyclostomes to mammals.