[Fatty acid makeup of various Brucella species and its relationship to the culture medium]. / Sostav zhirnykh kislot brutsell razlichnykh vidov i ego sviaz' so sredoi vyrashchivaniia.

Gas chromatographic method was applied to the study of the fatty acid composition (in Br. melitensis, Br. abortus, Br. suis, and Br. ovis strains. Fatty acid composition was similar in the mentioned brucellae species, except Br. suis No. 1330 significantly differing by this sign. Methyleneoctadecanoic acid content was considerably elevated, and that of octadecenoic -- reduced in brucellae grown on liver agar with the addition of serum and on meat-peptone agar in comparison with brucellae grown on liver agar; apparently this represents one of the mechanisms of the microorganism adaptation to the less favourable conditions of the nutrient medium. Passage of Br. ovis strain through the guinea pig organism led to the appearance of brucellae forming two types of colonies when grown on liver agar with the addition of serum. The fatty acid composition of brucellae forming small transparent colonies was the same as that of the initial culture with the prevalence of methyleneoctadecanoic acid; as to brucellae with larger colonies with irregular margin and nontransparent centre of the colony--octadecenoic acid prevailed in their fatty acid composition, i.e. their composition was similar to such in brucellae of the melitensis and abortus species grown on liver agar.

[Study of the antagonistic action of actinomycetes on anthrax bacilli]. / Izuchenie antagonisticheskogo deistviiaaktinomitsetov na batsilly sibirskoi iazvy

Capacity for the growth inhibition of the highly virulent causative agent of Siberia plague was studied with respect to actinomycetous strains from soil samples of the Ukraine. It was found that on nutrient media 88.4, 88.9, 88.1 and 93.4% of the isolates inhibited the growth of Bac. anthracis, Bac. cereus, Bac. mycoides and Tsenkovsky vaccine strain respectively. Representatives of very different taxonomic groups were found among the antagonistic actinomycetes. Clearance of the soil from Siberia plague bacilli stable to the outer effect with the help of the active strain L-721 of Act. chromofuscus was observed. The effect was chown in sterilized soil, not sterile soil and soil monolith.