[Physical rehabilitation for ankle joint injuries in the elderly and senile (stage 3).]

Ankle injuries are among the most common musculoskeletal injuries in the elderly. However, among the new scientific publications, both foreign and domestic, there are almost no examples and algorithms for conducting rehabilitation exercises for elderly patients, which prevents the introduction of these practices into rehabilitation measures. The purpose of our study is to evaluate the created technique of physical rehabilitation and, with a reliable result, to introduce it into the daily work of doctors of various profiles who provide assistance to patients with ankle joint injuries. The patients of the comparison group received standard complex therapy, including orthopedic methods of treatment, drug therapy, myostimulation, and massage. Patients of the main group, in addition to the traditional complex, was added to the exercise therapy technique according to the claimed method. For the entire period of the study, no complications and side effects were identified, which is an advantage compared to other methods of rehabilitation. The minimum costs, the general availability and autonomy of the training, the high results achieved during the experiment confirm the value and necessity of creating similar techniques for other injuries of the musculoskeletal system. The developed method of physical therapy exercises has shown its effectiveness and relevance among elderly patients according to goniometry data and can be recommended for use among other population groups as a rehabilitation method after ankle joint injuries.

Investigation of the genotoxic effects of fluoride on a bone tissue model.

Fluorides are thought to be a major cause of osteocarcinogenesis, due to their widespread industrial use, ability to accumulate in bone tissue, and genotoxic and probable carcinogenic properties. In vitro experiments investigating the genotoxic potential of fluorides in bone tissue models can provide valuable indirect information on their involvement in osteocarcinogenesis. Here, we investigated whether sodium fluoride (NaF) has the ability to induce DNA damage and chromosomal abnormalities in human osteosarcoma cells after 48 and 72 h of exposure. The cell cultures were treated with NaF in concentrations of 0, 20, 100 and 200 µg/ml. The level of DNA damage was assessed by the comet assay, and the frequency of chromosomal abnormalities by a micronucleus test. A significant increase in DNA damage indicators was noted in the samples treated with fluoride concentrations of 100 and 200 µg/ml, after 48 and 72 h of exposure. The micronucleus test revealed a dose-dependent increase in cells with micronuclei, nucleoplasmic bridges and nuclear protrusions. Increasing the concentration of NaF led to an increase in the prevalence of cytogenetic indicators after both treatment durations. This demonstrated ability of fluorine to exert genotoxic effects on bone cells indirectly indicates the possible importance of fluoride in the aetiology of osteosarcoma.