Effect of transplantation of immunocompetent cell on orientation and exploratory behavior and cytokine gene expression in the brain of experimental animals.

Intravenous injection of adherent splenocyte fraction from donor (CBAxC57Bl/6)F(1) mice characterized by specific open-field behavior modified this behavior in syngeneic recipient mice. This was paralleled by appropriate changes in the levels of IL-1b and type 1 IL-1 receptor mRNA in the brain cells of recipient mice. Hence, we demonstrated the possibility of directed regulation of orientation and exploratory behavior in mice by transplantation of immunocompetent cells. Mononuclear phagocytes play an important role in this phenomenon.

Immunomorphological characteristics of animals with different levels of orientation and exploratory behavior.

We revealed some relationships between the level of orientation and exploratory behavior, functional activity of the immune system, and structural and functional organization of the CNS in animals. Significant differences in brain morphology and expression of cytokine IL-1 beta, type I IL-1 receptor, and erythropoietin receptor genes in brain cells were detected in (CBA x C57Bl/6)F1 mice with different initial levels of orientation and exploratory behavior. Immunocompetent cells of mice with high and low levels of exploratory behavior differ by spontaneous and mitogen-induced proliferative activity. The formation of humoral and cellular immune response in these animals causes opposite changes in exploratory behavior and the type of these changes depends on the initial level of this behavioral reaction.

[Immunotherapy of lung tumors in Balb/c mice]. / Immunoterapiia opukholei lëgkikh u myshei Balb/c.

Tissue-specific immune reactions were used to develop a method of dealing with malignant tumors. Injection of proteins isolated from lung tissue of mice (CBAxC57BL/6)F1, of varying age, into mice Balb/c with urethane-induced lung tumors cut the number of such tumors by half. Direct correlation was found between the magnitude and nature of effect and level of lung protein injection.

Modulation of orientation and exploratory behavior in mice during development of humoral immune response.

We demonstrated opposite changes in the orientation and exploratory behavior of (CBAxC57Bl/6)F(1) mice in the open field test during the formation of primary humoral immune response. These changes depended on initial behavioral activity: the exploratory behavior was suppressed in animals with initially high activity, stimulated in animals with medium reaction, and remained unchanged in mice with initially low activity. The detected changes in the exploratory behavior during the formation of immune response were leveled in the total population (not divided by initial behavioral status).

Cytokine gene expression in cerebral hemispheres and behavioral reactions of (CBAxC57Bl)F1 mice.

Interleukin-1beta mRNA, interleukin-1beta receptor mRNA, and erythropoietin receptor mRNA are expressed in the brain of (CBAxC57Bl)F1 mice. Immunization with sheep erythrocytes stimulated the expression of these cytokines in the cerebral hemispheres. Injection of recombinant erythropoietin reduced expression of interleukin-1beta, interleukin-1beta receptor, and erythropoietin receptor genes in the brain of experimental animals and considerably increased their motor activity in the open field test.

Modulation of orientation and exploratory behavior of (CBAxC57Bl/6)F1 mice by immunocompetent cells.

The present investigation develops a previously published concept on integration of the immune, endocrine, and nervous systems in a universal structural and functional block (IMEN system). Splenocytes from donor (CBAxC57Bl/6)F1 mice characterized by specific behavior in the open field test modified the behavior of syngeneic recipient mice after intravenous infusion.

[Expression of erythropoietin receptor mRNA in mouse brain hemispheres]. / Ekspressiia mRNK retseptorov éritropoétina v polushariiakh golovnogo mozga myshei.

Now there is a growing evidence that erythropoietin receptors (Epo-R) are present also in some nonhematopoietic tissues such as endothelial cells and fetal cells of neural origin, although the physiological role of Epo-R at these sites is unclear. There are some speculations that Epo-R may be expressed on cells only in the developing CNS. The objective of this study was to determine whether Epo-R mRNA may be expressed in the brain hemispheres of Balb/c mice of different age groups: 1) newborn mice, 2) young 2 months old mice, 3) old 1.8 year old mice. We also studied the in vivo effect of recombinant erythropoietin on the expression of Epo-R mRNA in the brain hemispheres of (CBA x C57BL)F1 mice by RT-PCR. We have detected the existence of Epo-R mRNA expression in brain hemispheres of all the groups, but in old mice this expression was significantly higher. We have discovered a decrease in Epo-R mRNA expression in brain hemispheres of (CBA x C57BL)F1 mice 24 h after in vivo administration of recombinant erythropoietin. The Epo-R mRNA expression in the left brain hemispheres of (CBA x C57BL)F1 was considerably higher than in the right one.

Modulation of exploratory behavior in mice during activation of cell immune response.

We revealed a modulating effect of cell immune response on exploratory activity of (CBA degrees C57Bl/6)F1 mice depending on their initial behavioral profile. The formation of BCG-induced delayed-type hypersensitivity was accompanied by stimulation of the exploratory behavior in animals with initially low or intermediate activity, but did not change this parameter in behaviorally active mice.