Microorganisms of Two Thermal Pools on Kunashir Island, Russia.

The Kuril Archipelago is a part of the Circum-Pacific Belt (Ring of Fire). These islands have numerous thermal springs. There are very few studies on these microbial communities, and none of them have been conducted by modern molecular biological methods. Here we performed the first metagenomic study on two thermophilic microbial communities of Kunashir Island. Faust Lake is hot (48 °C) and highly acidic (pH 2.0). We constructed 28 metagenome-assembled genomes as well as 17 16S ribosomal RNA sequences. We found that bottom sediments of Faust Lake are dominated by a single species of red algae belonging to the Cyanidiaceae family. Archaeans in Faust Lake are more diverse than bacteria but less abundant. The Tretyakovsky Thermal Spring is also hot (52 °C) but only weakly acidic (pH 6.0). It has much higher microbial diversity (233 metagenome-assembled genomes; 93 16S ribosomal RNAs) and is dominated by bacteria, with only several archaeans and one fungus. Despite their geographic proximity, these two thermal springs were found to not share any species. A comparison of these two lakes with other thermal springs of the Circum-Pacific Belt revealed that only a few members of the communities are shared among different locations.

Metagenomics data of microbial communities in bacterial mats and bottom sediments in water bodies within the Kurai Mercury Province (Gorny Altai, Russia).

Below is data on the microbial diversity in bottom sediments and microbial mats in water bodies within the Kurai Mercury Province (Ulagan District, Aktash village, Gorny Altai). A database on the geochemical features of water bodies in the study area is presented. Data was obtained using 16 s rRNA amplicon directed metagenomic sequencing on Illumina MiSeq. The raw sequence data used for analysis is available in NCBI under the Sequence Read Archive (SRA) with BioProject No. PRJNA670076 and SRA accession numbers SRX9316205, SRX9316207, SRX9316208, SRX9316209.

Metagenomics data of microbial communities of natural organic matter from the dispersion train of sulfide tailings.

Below is data on the microbial diversity of natural organic matter from the Dispersion Train of Sulfide Tailings (northern Salaire Ridge, southwestern Siberia, Russia, Ursk Village). Data was obtained using 16s rRNA amplicon directed metagenomic sequencing on Illumina MiSeq. The raw sequence data used for analysis is available in NCBI under the Sequence Read Archive (SRA) with BioProject No. PRJNA670045 and SRA accession number SRX9314152, SRX9314376. The data sequences of the 16s rRNA gene are presented at the links MW142408-MW142413, MW142414-MW142447.

Metagenomic data on prokaryotic diversity of Kunashir island geothermal spring.

This is data on the microbial diversity of a geothermal spring located on the banks of the acidic creek of Kunashir Island. Data was obtained using 16s rRNA amplicon directed metagenomic sequencing on Illumina MiSeq. The raw sequence data used for analysis is available in NCBI under the Sequence Read Archive (SRA) with the BioProject No. PRJNA637298, PRJNA637447 and SRA accession number SRP265942, SRP266050. The data sequences of the 16s rRNA gene are presented at the accession numbers MT604934-MT604967, MT604911-MT604921 in NCBI GenBank database.

Metagenome-Assembled Genome of Halomonas sp. Isolate SL48-SHIP-3 from the Microbial Mat of Salt Lake Number 48 (Novosibirsk Region, Russia).

The Halomonas sp. isolate SL48-SHIP-3 genome was obtained from metagenomics sequencing of the microbial mat of Salt Lake Number 48 (54.201806N, 78.179194E; Novosibirsk region, Russia). The sequenced and annotated genome is 2,575,909 bp and encodes 2,368 genes.

Draft Genome Sequence of Halorubrum sp. Strain 48-1-W from a Saline Lake in the Novosibirsk Region, Russia.

Halorubrum sp. strain 48-1-W was isolated from a water sample from a saline lake (Novosibirsk Region, Russia, 54°14'N 78°13'E). The sequenced and annotated genome is 3,584,929 bp and contains 3,506 genes.

Draft Genome Sequence of Bacillus altitudinis Strain KU-skv2(2), Isolated from a Microbial Mat on an Anthropogenic Pipe from Caldera Uzon (Kamchatka, Russia).

Bacillus altitudinis strain KU-skv2(2) was isolated from a microbial mat on an anthropogenic pipe from Caldera Uzon (Kamchatka, Russia, 54°30'0.23″N, 160°0'15.18″E). The sequenced and annotated genome is 3,739,340 bp in size and encodes 3,929 genes.

Draft genome sequence of Anoxybacillus flavithermus KU2-6-11 isolated from hot-spring in Uzon caldera (Kamchatka, Russia).

The Anoxybacillus flavithermus KU2-6-11 was isolated from sediments of a nameless hot spring. The hot spring is located in Uzon caldera (Kamchatka, Russia). The sequenced and annotated genome is 2,646,305 bp and encodes 2787genes. The draft genome sequence of the Anoxybacillus flavithermus KU2-6-11 has been deposited at DDBJ/EMBL/GenBank under the accession PEDM01000000 and the sequences could be found at the site https://www.ncbi.nlm.nih.gov/nuccore/PEDM01000000.