RESUMO
An enzyme immunoassay (EIA) (Gonozyme, Abbott Laboratories, Chicago, IL) was evaluated for its ability to detect L-forms of Neisseria gonorrhoeae in pure and mixed culture suspensions. A total of 15 L-form strains that were induced from fresh clinical isolates of N. gonorrhoeae on an L-form medium were tested by EIA at antigen levels equivalent to those found in 2 X 10(5), 3 X 10(4), and 2 X 10(3) parental cells per milliliter. The results showed the sensitivity of the EIA for L-forms and parental cells to be the same, exhibiting positive results in all pure culture suspensions of parental cells at 2 X 10(5) and 3 X 10(4) cells per milliliter and their corresponding L-form preparations. At 2 X 10(3) cells per milliliter, three parental and two of their respective L-form preparations yielded positive EIA results. Incorporation of a mixture of heterologous organisms that can be found in the normal human genital flora, each at a concentration of 1 X 10(7) cells per milliliter into the L-form preparations, did not affect the sensitivity of the EIA for detection of L-forms. The results of the present study indicate that the EIA is an equally sensitive method for detecting gonococcal L-forms and parental cells. A further study, however, is needed to ascertain its usefulness under actual clinical conditions.
Assuntos
Técnicas Imunoenzimáticas , Neisseria gonorrhoeae/isolamento & purificação , Técnicas Bacteriológicas , Estudos de Avaliação como AssuntoRESUMO
The incidence of bleeding duodenal varices is exceedingly rare. We report a case of a 35-year-old chronic alcoholic who died of severe fatal upper gastrointestinal bleeding from a ruptured duodenal varix located in the second portion of duodenum. This diagnosis was suspected at endoscopy and was confirmed at autopsy. A review of literature shows 20 case reports of bleeding duodenal varices with two fatalities. While the duodenal bulb is the most common site of varices, the second portion of duodenum appears to be the next most common site. From all of the possible causes of duodenal varix, chronic liver diseases, mainly cirrhosis, remains the predominant etiological factor accounting for 30.7% of reported cases.
Assuntos
Duodeno/irrigação sanguínea , Hemorragia Gastrointestinal/etiologia , Hepatite Alcoólica/complicações , Cirrose Hepática Alcoólica/complicações , Pancreatite/patologia , Varizes/etiologia , Adulto , Doença Crônica , Duodeno/patologia , Hemorragia Gastrointestinal/patologia , Hepatite Alcoólica/patologia , Humanos , Cirrose Hepática Alcoólica/patologia , Masculino , Pancreatite/complicações , Ruptura Espontânea , Varizes/patologiaRESUMO
Reagent-impregnated filter paper replica methods are described which can be used to detect all penicillinase (beta-lactamase)-producing Neisseria gonorrhoeae (PPNG) colonies in an entire population of Neisseria gonorrhoeae growing on primary isolation plates.
Assuntos
Neisseria gonorrhoeae/enzimologia , beta-Lactamases/biossíntese , Técnicas Bacteriológicas , Resistência às Penicilinas , Fitas ReagentesAssuntos
Carcinógenos/metabolismo , Fluorenos/metabolismo , Neoplasias Experimentais/induzido quimicamente , Adenoma de Ducto Biliar/induzido quimicamente , Animais , Ascite/complicações , Neoplasias dos Ductos Biliares/induzido quimicamente , Neoplasias dos Ductos Biliares/patologia , Isótopos de Carbono , Cromatografia em Papel , Feminino , Glucuronatos/urina , Cirrose Hepática/induzido quimicamente , Cirrose Hepática/complicações , Cirrose Hepática/patologia , Masculino , Ratos , Ácidos Sulfúricos/urinaRESUMO
A single injection of 100 micrograms of estradiol benzoate into newborn rats was followed after weaning by dietary treatment with one of two dosages of the carcinogen N-hydroxy-N-2-fluorenylacetamide. Autopsies 26 weeks later showed a higher incidence of liver cancer in male and, particularly, female rats injected with hormone than in controls. The weights of livers were greater but gonads were smaller in size in the estradiol groups. Endocrine and possibly centralnervous-system factors may play roles in formation of liver tumors.