Effect of vermicompost on rhizobiome and the growth of wheat on Martian regolith simulant.

As humanity embarks on the journey to establish permanent colonies on Mars, ensuring a reliable source of sustenance will be crucial. Therefore, detailed studies regarding crop cultivation using Martian simulants are of great importance. This study aimed to grow wheat on substrates based on soil and Martian simulants, with the addition of vermicompost, to investigate the differences in wheat development. Basic physical and chemical properties of substrates were examined, including determination of macro- and microelements as well as their microbiological properties. Plant growth parameters were also determined. The addition of vermicompost positively affected wheat grown on soil, but the effect on plants grown on substrate with Martian simulants was negligible. Comparing the microbiological and chemical components, it was observed that plants can defend themselves against the negative effects of growth on the Martian simulants, but their success depends on having the PGPR (Plant growth-promoting rhizobacteria) present, which can provide the plant with additional nitrogen. The presence of beneficial symbiotic microbiota will allow the wheat to wait out the negative growth time rather than adapt to the regolith environment.

The impact of various forms of silver nanoparticles on the rhizosphere of wheat (Triticum aestivum L.) - Shifts in microbiome structure and predicted microbial metabolic functions.

The study investigated the effects of different silver nanoparticles (AgNPs) on the soil microbiome and wheat growth. For comparison purposes, a commercial fungicide and silver nitrate (AgNO3) were used. The results revealed three distinct groups of nanoparticles based on their impacts. Small-size AgNPs (10 nm) with a negative charge, as well as fungicide had limited effects on the microbiome, similar to the no-treatment control. Bigger in size (30-60 nm) and a negative charge AgNPs showed the most beneficial effects on soil microbiota shifts. These AgNPs increased the abundance of bacteria with beneficial traits such as nitrogen-fixing, urease, protease, and lignin degradation bacteria. The third type of AgNPs had a positive charge of nanostructure and influenced specific microbial populations, increasing the abundance of anaerobic and autotrophic groups of microorganisms, which could be assessed as a harmful shift for plants growth promotions and was similar to the AgNO3 treatment. Overall, the study emphasized the potential of AgNPs in agriculture not only as biocidal. The conducted study proved that AgNPs with bigger size/negative charge, used in low concentration can have a surprisingly stimulating effect on the positive characteristics of the rhizosphere microbiome. Moreover, the surface charge of AgNPs is a significant factor affecting microbial activity of wheat rhizosphere soil, which in this treatment is significantly similar to the AgNO3 treatment.

Concept of Sustainable Demolition Process for Brickwork Buildings with Expanded Polystyrene Foam Insulation Using Mealworms of Tenebrio molitor.

The traditional demolition process for brickwork buildings results in a significant volume of mixed debris. The debris consists of ceramic bricks (and other wall elements), mortar, thermal insulation (usually expanded polystyrene or rockwool), smaller steel elements, pieces of wood, and glass. Such mixed debris is difficult to recycle. Separating thermal insulation that is "glued" by cement mortar to brickwork is probably the most difficult and time-consuming task in processing mixed debris. This task can be performed in a very different and fully "automatized" manner using Tenebrio molitor mealworms. The mealworms remove expanded polystyrene from brickwork surfaces and transform it into frass. In the paper, a research program aiming to prove the concept of using the mealworms of Tenebrio molitor for processing mixed debris is presented. The tests were conducted using two models of a three-layered brickwork wall, which is very common in Europe. The proposed approached was successful. Both types of used expanded polystyrene foam (EPS) were fully removed from multilayer wall specimens. The possibilities and limitations of the proposed processing method were discussed and analyzed. The conducted research proved that it is feasible to clean brickwork debris from the EPS using Tenebrio molitor mealworms. Differences in the speed of cleaning process regarding the type of EPS were noted. More research is needed to scale the process, and to find the best method for using frass. By using Tenebrio molitor mealworms, one can make the demolition process much cleaner.

Polyethylene, polystyrene and lignocellulose wastes as mealworm (Tenebrio molitor L.) diets and their impact on the breeding condition, biometric parameters, metabolism, and digestive microbiome.

This study aimed to identify the extent to which a diet of oatmeal and polymers affects the development of mealworms, their microbiome, the biochemical activity of their digestive system, and their feed-metabolizing capacity. With a polystyrene diet, feed loss was most significant, as indicated by FTIR (Fourier-transform infrared spectroscopy) of frass, which showed that polystyrene was the only compound that was chemically modified. Compared to the control diet, mealworm larvae developed best on polyethylene regranulate (PE-reg), quickly transiting from one developmental stage to another with minor mass loss. A lignocellulose-based diet was the least beneficial for mealworm development. A polystyrene diet was most beneficial in terms of the protein content in larvae, but the contents and quality (usefulness as food) of fatty acids in the insects fed these wastes were significantly lower than in the control insects. For each diet, specific microbial cultures formed, and the presence of protozoa and various biochemical activities suggested different survival strategies and assimilation mechanisms facilitating survival. Despite profound changes in the microbiota and biochemistry of the digestive tract of mealworms fed waste-based diets, this study indicates their potential for utilizing PE-reg and polystyrene.

Changes in the gut microbiome and enzymatic profile of Tenebrio molitor larvae biodegrading cellulose, polyethylene and polystyrene waste.

Recent studies have demonstrated the ability of mealworm (Tenebrio molitor) for plastic degradation. This study is focused on changes in microbiome structure depending on diets. Microbial community obtained from oat and cellulose diet formed similar group, two kinds of polyethylene formed another group, while polystyrene diet showed the highest dissimilarity. The highest relative abundance of bacteria colonizing gut was in PE-oxodegradable feeding, nevertheless all applied diets were higher in comparison to oat. Dominant phyla consisted of Proteobacteria, Bacteroides, Firmicutes and Actinobacteria, however after PS feeding frequency in Planctomycetes and Nitrospirae increased. The unique bacteria characteristic for cellulose diet belonged to Selenomonas, while Pantoea were characteristic for both polyethylene diets, Lactococcus and Elizabethkingia were unique for each plastic diet, and potential diazotropic bacteria were characteristic for polystyrene diet (Agrobacterium, Nitrosomonas, Nitrospira). Enzymatic similarity between oatmeal and cellulose diets, was shown. All three plastics diet resulted in different activity in both, digestive tract and bacteria. The enzymes with the highest activity were included phosphatases, esterases, leucine arylamidase, ß-galactosidase, ß-glucuronidase, α-glucosidase, ß-glucosidase, chitinase, α-mannosidase and α-fucosidase. The activity of digestive tract was stronger than cultured gut bacteria. In addition to known polyethylene degradation methods, larvae may degrade polyethylene with esterase, cellulose and oatmeal waste activity is related with the activity of sugar-degrading enzymes, degradation of polystyrene with anaerobic processes and diazotrophs.