[PCR-detection of cytomegalovirus infection in oncologic and non-oncologic patients with organ and tissue transplantation]. / PTSR-diagnostika tsitomegalovirusnoi infektsii u onkologicheskikh i neonkologicheskikh bol'nykh, perenesshikh transplantatsiiu organov i tkanei.

Cytomegalovirus (CMV) infection often causes death after organ transplantation. Therefore, adequate prevention, diagnosis and treatment of this complication are of critical importance. The experience of establishing routine PCR-detection of CMV is presented. A widely accepted PCR protocol was used for clinical purposes. The strategy included 3 steps: 1) PCR amplification of CMV-sequences from leukocytes of patients; 2) visualization of PCR-product in polyacrylamide gel; 3) verification of the identity of PCR product by its hybridization to a membrane-bound specific probe. This approach allowed to detect CMV infection in 10 out of 28 bone marrow and in 4 out of 32 kidney recipients. The administration of specific antiviral treatment led to the elimination of CMV sequences from the blood.