Education background and monthly household income are factors affecting the knowledge, awareness and practice on haze pollution among Malaysians.

Transboundary haze pollution caused by periodic forest fires has initiated a serious negative implication to the economy, tourism sectors and public health in Southeast Asia. Due to the raising concerns of health effects of haze towards the Malaysian population, the present study was conducted to investigate the degree of awareness towards haze and the number of safety measures adopted by the Malaysian population during the haze crisis based on demographic characteristics. A cross-sectional questionnaire-based study was conducted from 4 to 29 January 2016 on 387 subjects in Klang Valley, Malaysia. The results showed that more than 90% of the respondents were aware of the hazards of haze pollution. Respondents with higher education background with diploma, undergraduate and postgraduate degree possessed greater awareness on the hazards of haze (p < 0.001) and practiced safety measures (p = 0.025) compared with those with lower education background with no proper schooling, primary schooling and secondary schooling. Among the subjects with higher household income (>RM 10,000/month) had higher awareness level on haze (p < 0.05) and adopted more safety measures (p < 0.05) compared with respondents with lower household income (

Synergistic Effects of Curcumin and Piperine as Potent Acetylcholine and Amyloidogenic Inhibitors With Significant Neuroprotective Activity in SH-SY5Y Cells via Computational Molecular Modeling and in vitro Assay.

Hallmarks of Alzheimer's disease (AD) pathology include acetylcholine (ACh) deficiency and plaque deposition. Emerging studies suggest that acetylcholinesterase (AChE) may interact with amyloid ß (Aß) to promote aggregation of insoluble Aß plaques in brains of patients. Current therapeutic options available for AD patients, such as AChE inhibitors, provide only symptomatic relief. In this study, we screened four natural compounds believed to harbor cognitive benefits-curcumin, piperine, bacoside A, and chebulinic acid. In the first section, preliminary screening through computational molecular docking simulations gauged the suitability of the compounds as novel AChE inhibitors. From here, only compounds that met the in silico selection criteria were selected for the second section through in vitro investigations, including AChE enzyme inhibition assay, 3-(4,5-dimenthylthiazol-2-yl)-2,5-dimethyltetrazolium bromide (MTT) assay, Thioflavin T (ThT) assay, and biochemical analysis via a neuronal cell line model. Of the four compounds screened, only curcumin (-9.6 kcal/mol) and piperine (-10.5 kcal/mol) showed favorable binding affinities and interactions towards AChE and were hence selected. In vitro AChE inhibition demonstrated that combination of curcumin and piperine showed greater AChE inhibition with an IC50 of 62.81 ± 0.01 µg/ml as compared to individual compounds, i.e., IC50 of curcumin at 134.5 ± 0.06 µg/ml and IC50 of piperine at 76.6 ± 0.08 µg/ml. In the SH-SY5Y cell model, this combination preserved cell viability up to 85%, indicating that the compounds protect against Aß-induced neuronal damage (p < 0.01). Interestingly, our results also showed that curcumin and piperine achieved a synergistic effect at 35 µM with an synergism quotient (SQ) value of 1.824. Synergistic behavior indicates that the combination of these two compounds at lower concentrations may provide a better outcome than singularly used for Aß proteins. Combined curcumin and piperine managed to inhibit aggregation (reduced ThT intensity at 0.432 a.u.; p < 0.01) as well as disaggregation (reduced ThT intensity at 0.532 a.u.; p < 0.01) of fibrillar Aß42. Furthermore, combined curcumin and piperine reversed the Aß-induced up-regulation of neuronal oxidative stress (p < 0.01). In conclusion, curcumin and piperine demonstrated promising neuroprotective effects, whereas bacoside A and chebulinic acid may not be suitable lead compounds. These results are hoped to advance the field of natural products research as potentially therapeutic and curative AD agents.

Bacopa monnieri, a Neuroprotective Lead in Alzheimer Disease: A Review on Its Properties, Mechanisms of Action, and Preclinical and Clinical Studies.

Alzheimer disease is a neurodegenerative disease that is signified by cognitive decline, memory loss, and erratic behavior. Till date, no cure for Alzheimer exists and the current Alzheimer medications have limited effectiveness. However, herbal medicines may slow down the disease's progression, which may hopefully reduce the number of cases in the years to come. Numerous studies have been done on characterizing the neuroprotective properties from plants belonging to Scrophulariaceae family, particularly Bacopa monnieri and its polyphenolic compounds known as bacosides. This review presents the findings on bacosides in therapeutic plants and their impact on Alzheimer disease pathology. These reports present data on the clinical, cellular activities, phytochemistry, and biological applications that may be used in new drug treatment for Alzheimer disease.

Mechanism involved in insulin resistance via accumulation of ß-amyloid and neurofibrillary tangles: link between type 2 diabetes and Alzheimer's disease.

The pathophysiological link between type 2 diabetes mellitus (T2DM) and Alzheimer's disease (AD) has been suggested in several reports. Few findings suggest that T2DM has strong link in the development process of AD, and the complete mechanism is yet to be revealed. Formation of amyloid plaques (APs) and neurofibrillary tangles (NFTs) are two central hallmarks in the AD. APs are the dense composites of ß-amyloid protein (Aß) which accumulates around the nerve cells. Moreover, NFTs are the twisted fibers containing hyperphosphorylated tau proteins present in certain residues of Aß that build up inside the brain cells. Certain factors contribute to the aetiogenesis of AD by regulating insulin signaling pathway in the brain and accelerating the formation of neurotoxic Aß and NFTs via various mechanisms, including GSK3ß, JNK, CamKII, CDK5, CK1, MARK4, PLK2, Syk, DYRK1A, PPP, and P70S6K. Progression to AD could be influenced by insulin signaling pathway that is affected due to T2DM. Interestingly, NFTs and APs lead to the impairment of several crucial cascades, such as synaptogenesis, neurotrophy, and apoptosis, which are regulated by insulin, cholesterol, and glucose metabolism. The investigation of the molecular cascades through insulin functions in brain contributes to probe and perceive progressions of diabetes to AD. This review elaborates the molecular insights that would help to further understand the potential mechanisms linking T2DM and AD.

Anatomy education in a changing medical curriculum in India: medical student feedback on duration and emphasis of gross anatomy teaching.

Authors report here a survey of medical student feedback on the effectiveness of two different anatomy curricula at Christian Medical College, Vellore, India. Undergraduate medical students seeking the Bachelor in Medicine and Bachelor in Surgery (M.B.B.S.) degrees were divided into two groups by the duration of their respective anatomy curriculum. Group 1 students had completed a longer, 18-month curriculum whereas Group 2 counterparts followed a shorter, 12-month curriculum. Students' responses to a questionnaire were studied. Analysis of feedback from Groups 1 and 2 contrasted the effectiveness of the two anatomy curricula. The coverage of gross anatomy was rated adequate or more than adequate by 98% of Group 1 and 91% of Group 2. A desire for greater emphasis on gross anatomy teaching was expressed by 24% of Group 1 and 50% of Group 2 (P = 0.000). Two-thirds of all students felt that the one-year program was not adequate, and 90% of Group 1 and 74% of Group 2 felt that clinically oriented anatomy teaching required more emphasis. Dissection was helpful or very helpful for 94% of Group 1 and 88% of Group 2. This study suggests that a better understanding of gross anatomy was gained from a course of longer duration (18 months with 915 contact hr vs. 12 months with 671 contact hr). Students who completed the longer anatomy course had greater appreciation of the need for clinically oriented anatomy teaching and dissection.

Estimation of length of calcaneum and talus from their bony markers.

Since hardly a report is available on estimation of length of calcaneum and talus from a fragment of them, a fresh study was made on a present day south Indian population. A total of 110 calcanei (55 right and 55 left), and 70 tali (35 right and 35 left), all unpaired, dry, and devoid of gross pathology, were used. Maximum anteroposterior length of the bone was measured in millimeter using an anthropometric board, and linear measurements of the other bony markers were measured in millimeter using a sliding caliper. Bony markers of calcaneum were maximum anteroposterior length, maximum transverse width, length, width and depth of groove on the sustentaculum tali, and length, width, and depth of the sulcus calcanei. Bony markers of talus were maximum anteroposterior length, maximum transverse width, length and width of articular surface for the lateral malleolus, length and width of articular surface for the medial malleolus, vertical width and transverse width of articular surface of the head, width and depth of groove for tendon of the flexor hallucis longus, and length, width, and depth of the sulcus tali. Simple regression suggested that maximum length of the calcaneum regressed significantly with maximum transverse width, length, width and depth of groove on the sustentaculum tali, and length, width, and depth of the sulcus calcanei and that maximum length of the talus regressed significantly with maximum transverse width, length and width of the lateral articular surface, length of the medial articular surface, vertical and transverse diameters of the head, and depth of the sulcus tali. Maximum length of calcaneum and talus is derived from the regression values, to predict the stature of the person from available stature equations in the literature.