[Study of endothelium-dependent blood oscillations in the human skin microcirculatory bed].

Cutaneous microcirculation parameters were studied with laser Doppler flowmetry in healthy volunteers. To investigate endothelial-dependent peripheral blood flow oscillations the iontophoresis of 1% acetylcholine solution was carried out. To estimate the contribution of rhythmical components in blood flow signal the continuous wavelet-transform spectral analysis was used. To reveal correlation between microcirculation parameters under study the correlation analysis was used. The microcirculation index was shown to be the factor producing cross-correlation dependences. The only positive significant correlation between the blood flow oscillation amplitude in the range of endothelial activity normalized to mean microcirculation index at rest and maximal microcirculation index during the iontophoresis of acetylcholine was revealed.

[Aging features in functioning of skin microvascular bed in humans].

The state of skin microcirculation system has been studied in healthy volunteers aged from 20 to 77, using laser Doppler flowmetry. To reveal the reaction of microvascular bed in response to short-term ischemia, occlusive test has been carried out. During experiment the age-induced reduction of microcirculation index has been observed at rest. In response to stopping occlusion the 3.5--4-aliquot increase of microcirculation index has been revealed during reactive post-occlusive hyperemia in all age groups. The data obtained suggest maintenance of high reserve resources of skin microcirculation in ageing in response to ischemia.

[Estimation of age-related changes in the regulation of peripheral blood flow in humans].

The age-dependent features in the state of skin microvascular bed has been studied with laser Doppler flowmetry in healthy volunteers of different age groups. To reveal the reaction of skin blood flow in response to short-term ischemia, the occlusive test has been carried out. To estimate the contribution of rhythmic components to blood flow signal, continuous wavelet-transform spectral analysis was used. Age-dependent increase of pulse-wave amplitude and decrease of respiratory wave amplitude reflecting age-dependent changes in functioning of arteriolar and venular links of microvascular bed have been observed at rest. In response to short-term ischemia the age-dependent reduction of reserve resources has been revealed in functioning of arteriolar link of microvascular bed. The reduction of activity of myogenic, neurogenic and endothelial regulation systems have been shown at rest in ageing.

[Modulation of human peripheral blood neutrophil apoptosis by ultraviolet C-range radiation and by gamma-irradiation]. / Moduliatsiia apoptoza neitrofilov perifericheskoi krovi cheloveka ul'trafioletovym izlucheniem diapazona C i gamma-izlucheniem.

It was found that the irradiation with in vitro UVC (254 nm) in the dose range of 6-600 J/m2 accelerates the apoptosis of human peripheral blood neutrophils in a dose-dependent manner, with saturation occurring at UVC doses of 250-300 J/m2. gamma-Irradiation with a dose of 2 Gy accelerates the apoptosis of neutrophils, whereas the irradiation with doses of 10 and 20 Gy suppresses it (by 9 h of cultivation). Lipopolysaccharide (1 microgram/ml) suppresses the UVC-induced apoptosis of neutrophils.

[Metabolic transformation affected by specific Salmonella O antigen in the lymphocytes of salmonellosis patients]. / Metabolicheskaia perestroika pod vozdeistviem spetsificheskogo O-antigena sal'monell v limfotsitakh bol'nykh sal'monellezom.

Metabolic transformation in the lymphocytes of salmonellosis patients, manifested by changes in the proportion of nucleic acids due to the stimulating action of Salmonella specific O antigen, was studied by the method of microspectral luminescent analysis. The level of single-helix nucleic acids in lymphocytes was shown to increase 6-8 hours after the stimulating action of specific O antigen, which was manifested by the increase of the alpha parameter from 0.21 to 0.68 in salmonellosis patients. This made it possible to confirm the diagnosis of salmonellosis in 92-95% of cases even at the early period of the disease when serodiagnosis could not yet be made.

[Significance of the indices of the metabolic and immunological reorganization of lymphocytes stimulated by a specific O-antigen in acute dysentery patients]. / Znachenie pokzatelei metabolicheskoi i immunologicheskoi perestroiki limfotsitov, stimulirovannykh spetsificheskim O-antigenom, u bol'nykh ostroi dizenteriei.

Immunological and metabolic shifts occurring in the lymphocytes of patients with acute dysentery under the influence of specific Shigella O-antigens have been studied during the infectious process. These shifts, whose manifestation depends on the severity of the disease, have been shown to be most pronounced on week 1 of the disease, gradually subsiding by week 3. The indices characterizing these shifts can be used for the evaluation of the severity of Shigella infection and its prognosis.