RESUMO
The breaking strength of different adhesive systems (AdheSE, Contax, Ecusit Primer Mono and Clearfil Se Bond) was studied on 60 teeth. The most breaking strength was demonstrated by system Clearfil Se Bond--29.434+/-0.350 MPa. The least breaking strength from the studied bondings was demonstrated by the system AdheSe--23.708+/-0.068 MPa. The breaking strength of the adhesive system Clearfil Se Bond was by 21% higher than the corresponding value of the system Contax (24.312+/-0.086 MPa) and by 11% higher than the corresponding value of the system Ecusit (26.504+/-0.143 MPa).
Assuntos
Cimentos Dentários/química , Análise do Estresse Dentário , Dentina/química , Resistência à TraçãoRESUMO
A method of reverse transcription followed by polymerase chain reaction (RT-PCR) has been implemented for the demonstration of the rabbit haemorrhagic disease virus (RHDV) genome in organ suspensions, leukocytes and excretions of infected rabbits. RT-PCR has been tested with 10 RHDV strains isolated at various geographic sites and times using a pair of primers coming from the gene region coding for the capsid protein VP60. The same primers were effective in the amplification of 4 of 5 European brown hare syndrome (EBHS) virus isolates. Non-radioactive labelling of PCR products with digoxigenin during the amplification and a system of colorimetric assessment of hybridization reactions between a biotin-labelled RHDV capture probe and the chains of labelled amplicons (PCR ELISA) were used for specific analyses of nucleic acid synthesis. The sensitivity of the alternative procedure of analysis of the dig-labelled PCR products with PCR ELISA was two logs10 higher than that of conventional electrophoresis in agarose gel stained with ethidium bromide. The results of the hybridization reactions, carried out under various stringency conditions, have confirmed the presumption that the genomic similarity between the amplified and the probed areas of the capsid protein VP60 gene was not uniform within all the tested caliciviruses. A higher degree of heterogeneity was observed between the isolates of EBHSV and RHDV.
Assuntos
Caliciviridae/genética , Colorimetria , Nucleotídeos de Desoxiuracil/metabolismo , Digoxigenina , Lagomorpha/virologia , Reação em Cadeia da Polimerase/métodos , RNA Viral/análise , Animais , Encéfalo/virologia , Infecções por Caliciviridae/veterinária , Infecções por Caliciviridae/virologia , Ensaio de Imunoadsorção Enzimática , Vírus da Doença Hemorrágica de Coelhos/genética , Intestinos/virologia , Rim/virologia , Leucócitos/virologia , Fígado/virologia , Pulmão/virologia , Hibridização de Ácido Nucleico , RNA Viral/sangue , RNA Viral/urina , DNA Polimerase Dirigida por RNA , Coelhos , Baço/virologiaRESUMO
Three strains of porcine reproductive and respiratory syndrome virus (PRRSV) were isolated in porcine lung macrophage (PLM) cultures from three swine herds. This has been the first successful isolation of PRRSV in the Czech Republic and the strains received the designations CAPM V-501, CAPM V-502 and CAPM V-503, respectively. All the three isolates in PLM were identified by immunofluorescence and immunoperoxidase tests and the strain CAPM V-502 also by electron microscopy using the ultrathin section technique. The strain CAPM V-502 has been adapted to the cell line MARC-145. Viral RNA in PLM cultures infected with any of the isolated PRRSV strains was demonstrated by RT-PCR targeted to the more conserved ORF 7 genomic region encoding the nucleocapsid protein. The assessment of PCR products in agarose gel revealed a uniform size of 394 bp in all the three isolates and the European prototype strain Lelystad used as positive control.
