RESUMO
Cultivation of Trichinella muscular larvae, purified by centrifugation in 20 ... 50% saccharose density gradient, in protein--free nutrient media at a dosage of 3.5-.10(3) lar./ml in the presence of insulin has made it possible to obtain a soluble antigen of Trichinella. It has been shown by means of electrophoresis in polyacrylamide gel that the soluble (secretory-excretory) antigen has three protein fractions while the somatic Trichinella antigen has 18 fractions. It has been shown that the soluble (secretory-excretory) antigen can be used for immobilization of erythrocytes on the surface that enables the sensitivity and specificity of serological methods for diagnosis of trichinellosis to be increased.
Assuntos
Antígenos de Helmintos/isolamento & purificação , Trichinella spiralis/imunologia , Animais , Antígenos de Helmintos/análise , Antígenos de Helmintos/metabolismo , Meios de Cultura , Eletroforese em Gel de Poliacrilamida , Testes de Hemaglutinação , Larva/imunologia , Larva/metabolismo , Músculos/parasitologia , Solubilidade , Fatores de Tempo , Trichinella spiralis/isolamento & purificação , Trichinella spiralis/metabolismoRESUMO
The major drawback of the routine methods for measuring the suspension concentrations with a microscope is the predominance of the examined area where the particles are concentrated beyond the microscope visual field. To make the examination more convenient, short, and accurate, it is recommended that the suspension particles contained in a certain volume be concentrated with the use of a camera, shaping them as a truncated cone on its smaller foot, equal to a single visual field of the microscope, and there these particles be scintillated.
Assuntos
Helmintíase/parasitologia , Helmintos/isolamento & purificação , Microscopia/instrumentação , Contagem de Ovos de Parasitas/instrumentação , Parasitologia/instrumentação , Animais , Desenho de Equipamento , Helmintíase/patologia , Suspensões , U.R.S.S.RESUMO
Intrastrain heterogeneity of muscular larvae of trichinellids has been revealed in experiments of free sedimentation and isopyknic division in the density gradient of saccharose, the presence of which is confirmed by the study of morphological characters and infection activity. The presence of intrastrain heterogeneity indicates the necessity of introduction of standard technique for studies of biological characters of different strains of trichinellids.
