RESUMO
A new method of preservation of nucleated cells in the electric refrigerator with xenon. After slow freezing and storage is even one day at -80 °C persists for more than 60% leukocytes. Cell membranes are resistant to the vital dye. In 85% of granulocytes stored baseline lysosomal-cationic protein, reduced lipid peroxidation and antioxidant activity. Cryopreservation of biological objects in inert gases is a promising direction in the practice of medicine and can be an alternative to the traditional method using liquid nitrogen.
Assuntos
Preservação de Sangue/métodos , Criopreservação/métodos , Granulócitos/citologia , Granulócitos/metabolismo , Xenônio/química , Antioxidantes/metabolismo , Humanos , Peroxidação de Lipídeos , Lisossomos/metabolismoRESUMO
NADPH oxidase activation was studied by detecting the reactive oxygen species (ROS) and the dynamics of neutrophil phagocytic activity in blood before freezing and one day after exposure to -20 degrees C in the presence of pectic polysaccharides. Pectins (excluding lemnan) were found to increase the phagocytic activity of neutrophils, promote the formation of ROS in these cells and enhance NADPH oxidase activity in the following order: bergenan, comaruman, lemnan, apiuman, and potamogetonan. Cryoprotective action of lemnan and comaruman was proposed to be related to their unique chemical structures.
Assuntos
Criopreservação , NADPH Oxidases/metabolismo , Neutrófilos/citologia , Pectinas/metabolismo , Fagocitose , Células Cultivadas , Criopreservação/métodos , Ativação Enzimática , Humanos , Neutrófilos/metabolismo , Espécies Reativas de Oxigênio/metabolismoRESUMO
Human blood serum exhibiting ß-adrenoceptor-sensitizing activity does not prevent manifestation of similar activity of histidine, tryptophan, tyrosine, mildronat, and preductal. This opens prospects for the use of analogues of endogenous ß-adrenoceptor-sensitizing agent in clinical practice.
Assuntos
Agonistas Adrenérgicos beta/farmacologia , Contração Muscular/efeitos dos fármacos , Miométrio/efeitos dos fármacos , Receptores Adrenérgicos beta/metabolismo , Soro/química , Animais , Relação Dose-Resposta a Droga , Epinefrina/farmacologia , Ciclo Estral/fisiologia , Feminino , Histidina/farmacologia , Humanos , Metilidrazinas/farmacologia , Contração Muscular/fisiologia , Miométrio/fisiologia , Concentração Osmolar , Cloreto de Potássio/farmacologia , Ratos , Triptofano/farmacologia , Tirosina/farmacologiaRESUMO
We studied changes in phagocytic activity of neutrophils and lymphocytes subjected to cold anabiosis. The proposed effective method for introduction of human blood leukocytes into cold anabiosis at moderately low temperature (-40 degrees C) in the presence of a cryopreserving agent does not require washout of the biological material after defrosting.
Assuntos
Preservação de Sangue , Criopreservação , Linfócitos/fisiologia , Neutrófilos/fisiologia , Fagocitose , Crioprotetores , Humanos , Técnicas In Vitro , Fatores de TempoRESUMO
We propose a simple, effective, and economic method for introducing human blood leukocytes in cold anabiosis at moderately low temperature (-50 degrees Celsius).