RESUMO
Production of domoic acid (DA), the responsible toxin for amnesic shellfish poisoning, was examined for 44 strains of Pseudo-nitzschia spp. isolated from Ofunato Bay, Japan. Only one strain which was identified as Pseudo-nitzschia multiseries produced DA in a level comparable to Canadian strains. No significant DA was detected in the rest of the strains, indicating that toxic P. multiseries does not bloom in a high density in the bay.
Assuntos
Eucariotos/metabolismo , Ácido Caínico/análogos & derivados , Toxinas Marinhas/metabolismo , Fármacos Neuromusculares Despolarizantes/química , Canadá , Divisão Celular , Cromatografia Líquida de Alta Pressão , Japão , Ácido Caínico/metabolismo , Espectroscopia de Ressonância Magnética , Frutos do Mar/toxicidadeRESUMO
Prorocentrum lima was found to be distributed on the surface of the algae, Sargassum confusum and Carpopeltis flabellata collected at the Sanriku coast, northern Japan. Chemical analysis of cultured cells revealed that Sanriku strains of P. lima produce okadaic acid, a toxin responsible for diarrhetic shellfish poisoning. The Sanriku strain grew well in T1 medium at 15 degrees C at which tropical strains do not grow, indicating that it is a local strain which adapts to cooler environments.
Assuntos
Dinoflagellida/química , Ácido Okadáico/metabolismo , Frutos do Mar , Adaptação Biológica , Animais , Cromatografia Líquida de Alta Pressão , Diarreia/etiologia , Japão , TemperaturaRESUMO
In spite of the routine use of hemoglobin Alc (HbAlc) value to guide diabetes therapy, substantial differences have been noted between results obtained by different instruments and laboratories. It was suggested that confusion between the terms used for HbA1c, i.e. between "total HbA1c' which includes labile content, and "stable HbA1c', which does not, may account for some of these inter-instrument and inter-laboratory differences. However, an undesirable difference was found even in measurements of only the stable component of HbAlc using the two most commonly used HPLC analyzers in our country. We found that a two-point calibration using lyophilized hemoglobin at lower and higher ranges was effective in matching up results of HbA1c measurement. This finding was concordant with a report by the Committee of the Japan Diabetes Society and other reports. Two methods of HbA1c immunoassay, one performed with the DCA 2000 automated analyzer and another performed using the Liquitech kit or a general automated analyzer, were evaluated and found to perform as well as HPLC analyzers in all respects except precision. The results derived from immunoassay were in good agreement with the calibrated results by HPLC, with (Liquitech) and without (DCA 2000) conversions.
