Agreement in Squat Jump Force-Time Characteristics Between Smith Machine and Free-Weight Squat Jump Force-Time Characteristics.

ABSTRACT: Kotani, Y, Lake, J, Guppy, SN, Poon, W, Nosaka, K, and Haff, GG. Agreement in squat jump force-time characteristics between Smith machine and free-weight squat jump force-time characteristics. J Strength Cond Res 37(10): 1955-1962, 2023-The purpose of this study was to determine whether squat jump (SJ) force-velocity (FV) and load-velocity (LV) profiles created using free-weights agree with profiles created with a Smith machine. Fifteen resistance-trained male subjects (age = 26.4 ± 2.5 years; height = 1.75 ± 0.09 m; body mass = 82.6 ± 13.4 kg) participated in this study. All subjects completed 2 familiarization and 2 experimental sessions using both the Smith machine and free-weight SJs each separated by 48 hours. During the experimental trials, progressively loaded SJs were performed in a quasi-randomized block order with loads between 21 kg and 100% of the subject's body mass. Agreement between exercise mode was determined with a weighted least products regression analysis. No fixed or proportional bias was noted between exercise modes when using peak velocity (PV) and mean velocity (MV) to create an FV profile. There was no fixed and proportional bias present for the LV profile when the profile was created with PV. When the LV profile was calculated from MV, fixed and proportional bias were present, indicating that MVs were significantly different between exercise modes. In addition, the free-weight FV and LV profiles exhibited poor to good relative and good to poor absolute reliability. Furthermore, when created using the Smith machine, both profiles exhibited poor to moderate relative and absolute reliability. Based on these data, caution should be used when interpreting LV and FV profiles created with these 2 methods.

The Agreement Between a Portable Contact-Mat and Force-Plates During Bilateral Vertical Jumps.

Force plates are commonly used when assessing vertical jumping performance but are not always affordable or practical tools for all testing situations. Twenty-four participants volunteered to take part in a study investigating the agreement between bilateral force plates and a new commercially available contact mat that records jump height, flight-time (FT), and FT of individual limbs during both countermovement (CMJ) and squat (SJ) jumps. Each participant performed six jumps of each type while standing on a contact mat placed upon a pair of in-ground force plates. When compared to the force plate via ordinary least products regression, the contact mat agreed with force plate CMJ and SJ jump height, individual limb FT during CMJs, and left-leg FT during SJs. The bilateral contact mat provided valid assessment of individual limb FT during CMJs, but not SJs. Practitioners can therefore use a bilateral contact mat interchangeably with bilateral force plates to measure SJ and CMJ performance.

The Reliability and Magnitude of Time-Dependent Force-Time Characteristics During the Isometric Midthigh Pull Are Affected by Both Testing Protocol and Analysis Choices.

ABSTRACT: Guppy, SN, Kotani, Y, Brady, CJ, Connolly, S, Comfort, P, and Haff, GG. Title: The reliability and magnitude of time-dependent force-time characteristics during the isometric mid-thigh pull are impacted by both testing protocol and analysis choices. J Strength Cond Res 36(5): 1191-1199, 2022-This study aimed to investigate whether the use of short-duration (SHORT) isometric midthigh pull (IMTP) trials resulted in greater reliability and magnitude of time-dependent force-time characteristics than traditionally performed IMTP trials (TRAD). Fourteen subjects with >6 months' training experience with the power clean volunteered to take part in the study. Subjects performed five ∼1-second IMTP trials (SHORT) and five 5-second IMTP trials (TRAD). SHORT resulted in substantially more reliable rate of force development (RFD) measures (intraclass correlation coefficient [ICC] = 0.97-0.99; coefficient of variation [CV] = 2.6-7.0%), particularly during time bands from force-onset to 150 ms, compared with TRAD when trials were selected for analysis based on peak force (ICC = 0.66-0.83; CV = 14.1-38.5%). Selecting TRAD trials based on RFD0-200 resulted in similar reliability compared with SHORT of those same epochs (ICC = 0.97-0.99; CV = 2.5-7.8%). Furthermore, SHORT resulted in significantly greater force at specific time points, RFD, and impulse compared with TRAD trials (p = 0.001-0.033; g = -0.16 to -0.66). Based on these results, strength and conditioning professionals should use specific testing protocols (i.e., TRAD and SHORT) depending on the component of an athlete's force-generating capacity that they wish to assess and remain aware of the effect analysis choices they have on the reliability of IMTP force-time characteristics.

Reliability of the Squat Jump Force-Velocity and Load-Velocity Profiles.

ABSTRACT: Kotani, Y, Lake, J, Guppy, SN, Poon, W, Nosaka, K, Hori, N, and Haff, GG. The reliability of the squat jump force-velocity and load-velocity profiles. J Strength Cond Res 36(11): 3000-3007, 2022-The purpose of this study was to investigate the between-session reliability of the squat jump force-velocity (FV) and load-velocity (LV) profiles. Eighteen subjects (age = 28.1 ± 4.8 years; height = 1.7 ± 9.7; body mass = 74.7 ± 12.8) who could back squat >1.5 times body mass participated in this study. Each subject completed a familiarization session, followed by 2 experimental sessions each separated by 72 hours. Subjects performed a series of squat jumps on a force plate against external loads between 0 and 100% of their body mass in a quasi-randomized block order. Intraclass correlation coefficient (ICC) and coefficient of variation (CV) were used to examine the between-session reliability. Peak velocity (PV) and mean velocity (MV) at each load were highly reliable (ICC >0.80, CV% <7.41, SEM <0.13 m·s -1 , smallest detectable difference [SDD] <0.31 m·s -1 , ES <0.21). Force-velocity profiles created with peak force and relative peak force resulted in poor to excellent reliability (ICC = 0.34-0.92, CV% = 11.9-26.3). When mean and relative mean forces were used to create FV profiles, there was poor to good reliability (ICC = 0.03-0.85, CV% = 18.1-39.4). When the LV profile was calculated with PV (ICC = 0.60-0.90, CV% = 7.9-16.9) or MV (ICC = 0.49-0.91, CV% = 11.1-23.4), there was poor to excellent reliability. There was no time effect found between sessions for both FV and LV profiles. The squat jump FV and LV profiles established with a force plate are not reliable. Therefore, these profiles are not recommended to be used to inform programming decisions.

A comparison of manual and automatic force-onset identification methodologies and their effect on force-time characteristics in the isometric midthigh pull.

The aim of this study was to assess the agreement of three different automated methods of identifying force-onset (40 N, 5 SDs, and 3 SDs) with manual identification, during the isometric mid-thigh pull (IMTP). Fourteen resistance-trained participants with >6 months experience training with the power clean volunteered to take part. After three familiarisation sessions, the participants performed five maximal IMTPs separated by 1 min of rest. Fixed bias was found between 40 N and manual identification for time at force-onset. No proportional bias was present between manual identification and any automated threshold. Fixed bias between manual identification and automated was present for force at onset and F150. Proportional but not fixed bias was found for F50 between manual identification and all automated thresholds. Small to moderate differences (Hedges g = -0.487- -0.692) were found for F90 between all automated thresholds and manual identification, while trivial to small differences (Hedges g = -0.122--0.279) were found between methods for F200 and F250. Based on these results, strength and conditioning practitioners should not use a 40 N, 5 SDs, or 3 SDs threshold interchangeably with manual identification of force-onset when analysing IMTP force-time curve data.

Effect of Altering Body Posture and Barbell Position on the Within-Session Reliability and Magnitude of Force-Time Curve Characteristics in the Isometric Midthigh Pull.

Guppy, SN, Brady, CJ, Kotani, Y, Stone, MH, Medic, N, and Haff, GG. Effect of altering body posture and barbell position on the within-session reliability and magnitude of force-time curve characteristics in the isometric midthigh pull. J Strength Cond Res 33(12): 3252-3262, 2019-A large degree of variation in the position used during isometric midthigh pull (IMTP) testing and conflicting results of the effects of these changes can be found in the literature. This study investigated the effect of altering body posture and barbell position on the reliability and magnitude of force-time characteristics generated during the IMTP. Seventeen strength-power athletes (n = 11 males, height: 177.5 ± 7.0 cm, body mass: 90.0 ± 14.1 kg, age: 30.6 ± 10.4 years; n = 6 females, height: 165.8 ± 11.4 cm; body mass: 66.4 ± 13.9 kg, age: 30.8 ± 8.7 years) with greater than 6 months of training experience in the clean (1 repetition maximum: 118.5 ± 20.6 kg, 77.5 ± 10.4 kg) volunteered to undertake the experimental protocol. Subjects performed the IMTP using 4 combinations of hip and knee angles, and 2 different barbell positions. The first barbell position corresponded to the second pull of the clean, while the second rested at the midpoint between the iliac crest and the patella. Peak force (PF), time-specific force (F50, F90, F150, F200, and F250), peak rate of force development (pRFD), and impulse (IMP) time bands were reliable in all 4 testing positions examined. Statistically greater PF, F50, F90, F150, F200, F250, pRFD, and IMP0-50, IMP0-90, IMP0-150, and IMP0-200 were generated in a testing position corresponding to the second pull of the clean when compared with a bent over torso angle, regardless of the barbell position used. Moderate to large effect sizes favoring a testing position corresponding to the second pull were also found. Overall, when performing the IMTP, an upright torso and a barbell position that matches the second pull of the clean should be used.

The Effect of Altering Body Posture and Barbell Position on the Between-Session Reliability of Force-Time Curve Characteristics in the Isometric Mid-Thigh Pull.

Seventeen strength and power athletes (n = 11 males, 6 females; height: 177.5 ± 7.0 cm, 165.8 ± 11.4 cm; body mass: 90.0 ± 14.1 kg, 66.4 ± 13.9 kg; age: 30.6 ± 10.4 years, 30.8 ± 8.7 years), who regularly performed weightlifting movements during their resistance training programs, were recruited to examine the effect of altering body posture and barbell position on the between-session reliability of force-time characteristics generated in the isometric mid-thigh pull (IMTP). After participants were familiarised with the testing protocol, they undertook two testing sessions which were separated by seven days. In each session, the participants performed three maximal IMTP trials in each of the four testing positions examined, with the order of testing randomized. In each position, no significant differences were found between sessions for all force-time characteristics (p = >0.05). Peak force (PF), time-specific force (F50, F90, F150, F200, F250) and IMP time-bands (0⁻50, 0⁻90, 0⁻150, 0⁻200, 0⁻250 ms) were reliable across each of the four testing positions (ICC ≥ 0.7, CV ≤ 15%). Time to peak force, peak RFD, RFD time-bands (0⁻50, 0⁻90, 0⁻150, 0⁻200, 0⁻250 ms) and peak IMP were unreliable regardless of the testing position used (ICC = <0.7, CV = >15%). Overall, the use of body postures and barbell positions during the IMTP that do not correspond to the second pull of the clean have no adverse effect on the reliability of the force-time characteristics generated.

Proposed selection strategy of surrogate matrix to quantify endogenous substances by Japan Bioanalysis Forum DG2015-15.

It is important to select an appropriate surrogate matrix for preparing calibration standards and quality control samples while quantitatively assaying for endogenous substances, because a blank matrix that does not contain the endogenous substance cannot be derived from the species from which the target study samples are collected. This is because the assay results might be affected, depending on the characteristics of the analyte in the surrogate matrix. Our discussion group that participated in the Japan Bioanalysis Forum discussed the recommended selection strategies, focusing on large and small molecules in ligand binding assays and LC-MS, respectively. We established an efficient selection strategy for a surrogate matrix, with simple compositions as the first candidates stated in this article.

Parasporin-2Ab, a newly isolated cytotoxic crystal protein from Bacillus thuringiensis.

A novel crystal protein that exhibited potent cytotoxicity against human leukemic T-cells was cloned from the Bacillus thuringiensis TK-E6 strain. The protein, designated as parasporin-2Ab (PS2Ab), was a polypeptide of 304 amino acid residues with a predicted molecular weight of 33,017. The deduced amino acid sequence of PS2Ab showed significant homology (84% identitiy) to parasporin-2Aa (PS2Aa) from the B. thuringiensis A1547 strain. Upon processing of PS2Ab with proteinase K, the active form of 29 kDa was produced. The activated PS2Ab showed potent cytotoxicity against MOLT-4 and Jurkat cells and the EC(50) values were estimated as 0.545 and 0.745 ng/mL, respectively. The cytotoxicity of PS2Ab was significantly higher than that of PS2Aa reported elsewhere. Although both cytotoxins were structurally related, it was thought that the minor differences found were responsible for the different cytotoxicities of PS2Ab and PS2Aa.