Comparison of the thin-layer chromatographic properties of sulfur-containing amino acids and their aminophosphonic analogues.

The thin-layer chromatographic behavior of the natural, sulfur-containing amino acids (Cys, Hcys, Met) and their phosphonic analogues (CysP, HcysP, MetP) has been studied. Their detection limits using iodine and molybdate, ninhydrine and iodine-azide reagents were determined, and the R(F) values of these two classes of amino acids in acidic, neutral and mild basic solvent systems were compared.