RESUMO
OBJECTIVE: To establish the model of cellular inflammatory responses of gingival epithelial cells in vitro induced by Porphyromonas gingivalis vesicle and to probe into the pathogenesis of Porphyromonas gingivalis in periodontitis. METHODS: The effect of Porphyromonas gingivalis vesicle on prostaglandin E(2) (PGE(2)) production of gingival epithelial cells was detected by ELISA and the effects of Porphyromonas gingivalis vesicle on cyclooxygenase-2 (COX-2), interleukin (IL)-6 and IL-8 mRNA expression in gingival epithelial cells were determined by Real-time reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Porphyromonas gingivalis vesicle dose-dependently induced PGE(2) production and up-regulated COX-2, IL-6 and IL-8 mRNA expression in gingival epithelial cells significantly. CONCLUSIONS: Cellular inflammatory responses of gingival epithelial cells induced by Porphyromonas gingivalis vesicle may contribute to the initiation and progression of periodontitis.
Assuntos
Células Epiteliais/imunologia , Gengiva/imunologia , Porphyromonas gingivalis/imunologia , Aderência Bacteriana , Células Cultivadas , Ciclo-Oxigenase 2/imunologia , Ciclo-Oxigenase 2/metabolismo , Dinoprostona/imunologia , Dinoprostona/metabolismo , Células Epiteliais/metabolismo , Células Epiteliais/microbiologia , Gengiva/metabolismo , Gengiva/microbiologia , Humanos , Interleucina-6/imunologia , Interleucina-6/metabolismo , Interleucina-8/imunologia , Interleucina-8/metabolismo , Porphyromonas gingivalis/patogenicidadeRESUMO
PURPOSE: To study the effects of EGF on Ca(2+) and cAMP in the ductal cells of rat SMG. METHODS: Fluorometric technique and radioimmunological technique were used to investigate the dynamic changes of Ca(2+) and cAMP in the ductal cells. The data were analysed statistically by t test. RESULTS: After an momently latent period, intercellular calcium increased rapidly, then began to decline. This period could last 5 minutes. After 3 minutes, cAMP showed a rapid decrease, and then increased slowly. Its concentration could restore 90 minutes later. CONCLUSION: EGF may regulate intercellular calcium and cAMP, which results in the proliferation of the ductal cells.