Allometric relationships for eight species of 4-5 year old nitrogen-fixing and non-fixing trees.

Allometric equations are often used to estimate plant biomass allocation to different tissue types from easier-to-measure quantities. Biomass allocation, and thus allometric equations, often differs by species and sometimes varies with nutrient availability. We measured biomass components for five nitrogen-fixing tree species (Robinia pseudoacacia, Gliricidia sepium, Casuarina equisetifolia, Acacia koa, Morella faya) and three non-fixing tree species (Betula nigra, Psidium cattleianum, Dodonaea viscosa) grown in field sites in New York and Hawaii for 4-5 years and subjected to four fertilization treatments. We measured total aboveground, foliar, main stem, secondary stem, and twig biomass in all species, and belowground biomass in Robinia pseudoacacia and Betula nigra, along with basal diameter, height, and canopy dimensions. The individuals spanned a wide size range (<1-16 cm basal diameter; 0.24-8.8 m height). For each biomass component, aboveground biomass, belowground biomass, and total biomass, we determined the following four allometric equations: the most parsimonious (lowest AIC) overall, the most parsimonious without a fertilization effect, the most parsimonious without canopy dimensions, and an equation with basal diameter only. For some species, the most parsimonious overall equation included fertilization effects, but fertilization effects were inconsistent across fertilization treatments. We therefore concluded that fertilization does not clearly affect allometric relationships in these species, size classes, and growth conditions. Our best-fit allometric equations without fertilization effects had the following R2 values: 0.91-0.99 for aboveground biomass (the range is across species), 0.95 for belowground biomass, 0.80-0.96 for foliar biomass, 0.94-0.99 for main stem biomass, 0.77-0.98 for secondary stem biomass, and 0.88-0.99 for twig biomass. Our equations can be used to estimate overall biomass and biomass of tissue components for these size classes in these species, and our results indicate that soil fertility does not need to be considered when using allometric relationships for these size classes in these species.

N supply mediates the radiative balance of N2 O emissions and CO2 sequestration driven by N-fixing vs. non-fixing trees.

Forests are a significant CO2 sink. However, CO2 sequestration in forests is radiatively offset by emissions of nitrous oxide (N2 O), a potent greenhouse gas, from forest soils. Reforestation, an important strategy for mitigating climate change, has focused on maximizing CO2 sequestration in plant biomass without integrating N2 O emissions from soils. Although nitrogen (N)-fixing trees are often recommended for reforestation because of their rapid growth on N-poor soil, they can stimulate significant N2 O emissions from soils. Here, we first used a field experiment to show that a N-fixing tree (Robinia pseudoacacia) initially mitigated climate change more than a non-fixing tree (Betula nigra). We then used our field data to parameterize a theoretical model to investigate these effects over time. Under lower N supply, N-fixers continued to mitigate climate change more than non-fixers by overcoming N limitation of plant growth. However, under higher N supply, N-fixers ultimately mitigated climate change less than non-fixers by enriching soil N and stimulating N2 O emissions from soils. These results have implications for reforestation, suggesting that N-fixing trees are more effective at mitigating climate change at lower N supply, whereas non-fixing trees are more effective at mitigating climate change at higher N supply.

Nitrogen-fixing trees increase soil nitrous oxide emissions: a meta-analysis.

Nitrogen-fixing trees are an important nitrogen source to terrestrial ecosystems. While they can fuel primary production and drive carbon dioxide sequestration, they can also potentially stimulate soil emissions of nitrous oxide, a potent greenhouse gas. However, studies on the influence of nitrogen-fixing trees on soil nitrous oxide emissions have not been quantitatively synthesized. Here, we show in a meta-analysis that nitrogen-fixing trees more than double soil nitrous oxide emissions relative to non-fixing trees and soils. If planted in reforestation projects at the global scale, nitrogen-fixing trees could increase global soil nitrous oxide emissions from natural terrestrial ecosystems by up to 4.1%, offsetting climate change mitigation via reforestation by up to 4.4%.

Nitrogen-fixing trees could exacerbate climate change under elevated nitrogen deposition.

Biological nitrogen fixation can fuel CO2 sequestration by forests but can also stimulate soil emissions of nitrous oxide (N2O), a potent greenhouse gas. Here we use a theoretical model to suggest that symbiotic nitrogen-fixing trees could either mitigate (CO2 sequestration outweighs soil N2O emissions) or exacerbate (vice versa) climate change relative to non-fixing trees, depending on their nitrogen fixation strategy (the degree to which they regulate nitrogen fixation to balance nitrogen supply and demand) and on nitrogen deposition. The model posits that nitrogen-fixing trees could exacerbate climate change globally relative to non-fixing trees by the radiative equivalent of 0.77 Pg C yr-1 under nitrogen deposition rates projected for 2030. This value is highly uncertain, but its magnitude suggests that this subject requires further study and that improving the representation of biological nitrogen fixation in climate models could substantially decrease estimates of the extent to which forests will mitigate climate change.