[Assessment of biological sampling's quality in a medical laboratory: case of Côte d' Ivoire Institute Pasteur]. / Evaluation de la qualite des prelevements de patients ambulatoires dans un laboratoire de bacteriologie medicale : cas de l'Institut Pasteur de Cote d'Ivoire.

BACKGROUND: Assurance quality is important in medical laboratory, but in Africa, few laboratories are involved in this process. The aim of this study was to assess biological sampling's quality in a bacteriological laboratory. MATERIAL AND METHODS: A cross sectional study was undertaken in medical bacteriological laboratory of Côte d' Ivoire Institute Pasteur during 6 months. All urines, saddles, and bronchial expectorations collected from ambulatory patients during this period were included in the study. The quality of urine's, saddles and bronchial expectorations' sampling for a bacteriological analysis was evaluated. An interview based on Guidelines of good laboratories practices and referential ISO 15189 was used. A total of 300 samples were indexed. RESULTS: On a total of 300 recorded biological samples, 224 (74.7%) were not in conformity. In 87.5% of the cases of nonconformities, an antibiotic's treatment were preliminary instituted before the sampling. Corrective actions were carried in the laboratory on 30 samples with 56.6% for the urines, 26.7% for the saddles and 16.7% for the bronchial expectorations. CONCLUSION: At the end of this study, it arises that the quality of the biological sampling received at the medical bacteriology laboratory need to be improved.

[Neonatal urinary tract infections in Abidjan: the problem of bacterial resistance]. / Les infections urinaires neonatales a Abidjan: problematique de la resistance bacterienne.

BACKGROUND: Neonatal infection is frequent with rate of lethality between 20 to 70% in the developing countries. Among these neonatal infections, urinary infection is serious .The diagnosis of urinary infection is based on cytobacteriological exam. But it is rarely done for economic reasons. Principal objective of this survey was to identify the germs responsible for the urinary infections at the newborn in order to propose an adequate therapeutic algorithm. STUDY: From July to December 2004, 720 newborns hospitalized in neonatalogy for neonatal infection were enrolled. Specimens of urines were collected in view of cytobacteriological exam. Culture was positive for 136 samples (18,9%). About etiology of urinary infections, enterobacteria were predominant with 77,5% against 26,5% for cocci Gram positive. The frequency of isolation of the enterobacteria was following: Escherichia coli, Klebsiella pneumoniae, Enterobacter and Levinea with respectively 32%, 28%, 28% and 12%. The rates of global resistance of the enterobacteria to the usual antibiotics varied from 66,7% to 85,7% for the amoxicilline associated to the clavulanic acid; of 25% to 66,7% for the ceftriaxone; and of 0 to 33,3% for the amikacine.