RESUMO
The chromosomal damage (micronuclei) of the peripheral blood lymphocytes of the group of professionals who had participated in the liquidation of the Chernobyl NPP accident and have continued their work at the "Shelter" and of the group of self-settlers who living for about ten year in the Chernobyl NPP alienation zone were studied. Residents of Kyïv were the group for comparison. It was found that in professionals the number of micronuclei was higher due to the presence of cells with several micronuclei while in the self-settlers it was due to the increase in the number of cells with one micronucleus. Also was determined the correlation between the frequency of micronuclei in the lymphocytes of the self-settlers on the one hand and the level of radionuclides contamination of territory and dose loading on the other.
Assuntos
Emigração e Imigração , Exposição Ambiental/efeitos adversos , Linfócitos/efeitos da radiação , Micronúcleos com Defeito Cromossômico/efeitos da radiação , Exposição Ocupacional/efeitos adversos , Centrais Elétricas , Liberação Nociva de Radioativos , Adulto , Aberrações Cromossômicas , Exposição Ambiental/estatística & dados numéricos , Feminino , Humanos , Masculino , Testes para Micronúcleos/estatística & dados numéricos , Pessoa de Meia-Idade , Exposição Ocupacional/estatística & dados numéricos , Doses de Radiação , UcrâniaRESUMO
Some metabolic peculiarities of bacteria of the genus Klebsiella were investigated. The bacteria under study were isolated from different sources and varied in virulence. The pathogenic and saprotrophic enterobacteria were discriminated based on their response to the addition of carbohydrates or nitrate to the medium. Pathogenic Klebsiella spp. exhibited mainly the mixed formic-acid type of fermentation and were more resistant to nitrates than saprotrophic bacteria with the butanediol type of fermentation. The bacteria Klebsiella pneumoniae subsp. pneumoniae isolated from different sources, such as patients, healthy persons, or the environment, exhibited no substantial differences in metabolism and virulence. It was inferred that these bacteria themselves cannot cause disease, and their isolation in morbid states is an indirect result of dysbacteriosis.
Assuntos
Klebsiella/metabolismo , Metabolismo dos Carboidratos , Fermentação , Klebsiella/patogenicidade , Nitratos/metabolismo , Especificidade da Espécie , VirulênciaRESUMO
While comparatively analyzing the properties of Klebsiella pneumoniae from three studied sources (patients, healthy people, environment), no reliable differences between the comparable strains have been revealed. No differences have been found in the toxin formation, hemagglutinating and antilysozymic activity, adhesivity and invasive properties as well as in the peculiarities of gas exchange, while growing on media with different nitrogen and carbon sources. The studied microorganisms differed only in antibiotic resistance: strains isolated from the environment were sensitive to antibiotics, whereas strains of human origin were resistant to them.
Assuntos
Klebsiella pneumoniae/fisiologia , Antibacterianos/farmacologia , Aderência Bacteriana , Células Cultivadas/microbiologia , Meios de Cultura , Microbiologia Ambiental , Humanos , Infecções por Klebsiella/microbiologia , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/isolamento & purificação , Klebsiella pneumoniae/patogenicidade , Testes de Sensibilidade Microbiana , Muramidase/antagonistas & inibidores , Fatores de TempoRESUMO
Irradiation conditions in which laboratory animals were kept in experimental laboratories of Chernobyl and Kiev after the accident at the Chernobyl A.P.S. are described. The data are presented on the spectral structural and activity of radionuclides in the diet as well as in the organs and tissues of the animals. The radiation loads have been estimated with regard to an external gamma component and the internal one contributed by the incorporated radionuclides. It has been shown that radiation doses received by the animals during their lifetime due to these contributions do not exceed units of cGy.
Assuntos
Acidentes , Exposição Ambiental , Reatores Nucleares , Centrais Elétricas , Efeitos da Radiação , Poluentes Radioativos do Ar/efeitos adversos , Poluentes Radioativos do Ar/análise , Animais , Contaminação Radioativa de Alimentos , Camundongos , Camundongos Endogâmicos C57BL , Doses de Radiação , Monitoramento de Radiação , Radioisótopos/análise , Radioisótopos/farmacocinética , Ratos , UcrâniaRESUMO
It has been shown that rats born during the first months after the Chernobyl A.P.S. disaster exhibit essential changes in the peripheral blood and bone marrow haemopoiesis throughout the entire lifetime. Rats brought up in Chernobyl from the age of three months on display even more pronounced changes. It is assumed that the changes in the haemopoiesis develop due to the continuous influence of low-level radiation of different quality and are attributed to the effect of the incorporated radionuclides.