RESUMO
BACKGROUND: Oxidative stress (OS) may result in damage to critical macromolecules, and an association between lead (Pb) toxicity and OS is a matter of research. Therefore, the aim of the present study was to investigate the effect of Pb on the oxidative system in indirectly Pb-exposed male apprentices. METHODS: Established parameters of Pb toxicity (aminolevulinic acid dehydratase index [ALAD index], zinc-protoporphyrin [ZPP]) as well as Pb-level in blood were determined in Pb-exposed apprentices (n > 25) and controls (n > 24). Enzymatic (glutathione peroxides [GPx], superoxide dismutase [SOD], catalase [CAT]) and non-enzymatic (alpha-tocopherol, beta-carotene) indices of OS, and malondialdehyde (MDA) level were also determined. RESULTS: There was a statistically significant increase in Pb level, ALAD index, ZPP concentration, GPx activity and MDA concentration in Pb-exposed apprentices when compared to controls. Although a statistically significant decrease in alpha-tocopherol and beta-carotene levels was seen, SOD and CAT activities were unaltered in Pb-exposed apprentices. Pb level and duration of Pb exposure were correlated with each other, as well as various indices of OS and MDA concentration. CONCLUSIONS: Chronic indirect Pb exposure results in lipid peroxidation in erythrocytes of apprentices via OS, and duration of Pb exposure is a reliable marker of Pb toxicity.
