RESUMO
This article deals with the autofluorescence spectra from the hard tissues of a tooth, both in norm and pathology. An investigation was made on 30 extracted human teeth. The measurements were made both for the intact hard tissues of a tooth, such as enamel, dentine, cementum, and root canal, and for the tissues pathologically affected by a caries (superficial, intermediate, and deep) and by a dental calculus. It was found that the fluorescent spectra from enamel, dentine, cementum, and from the regions affected by a caries and dental calculus were identical in form. All the spectra revealed a maximum near 700 nm. However, the intact and affected hard tissues were greatly different in the integral fluorescent intensity. Dental calculus was found to produce the most pronounced fluorescent intensity, whereas the carious regions produced a slightly weaker fluorescent intensity. On the contrary, the intact hard tissues of a tooth exhibited the poorest fluorescent intensity.
Assuntos
Espectrometria de Fluorescência/métodos , Doenças Dentárias/diagnóstico , Doenças Dentárias/metabolismo , Dente/química , Fenômenos Biofísicos , Biofísica , Cálculos Dentários/química , Cálculos Dentários/diagnóstico , Cárie Dentária/diagnóstico , Cárie Dentária/metabolismo , Cemento Dentário/química , Esmalte Dentário/química , Cavidade Pulpar/química , Dentina/química , Humanos , Técnicas In VitroRESUMO
Possible application is studied for fluorescence spectroscopy in the express-evaluation of the gastrointestinal microflora. This diagnostic approach is feasible because of the difference in fluorescent spectra from different microorganisms, with anaerobic microorganisms exhibiting the most pronounced fluorescence. It was found that fluorescence strength raised with an increase in the bacteria Bacteroides and bifid bacteria, whereas it decreased with an increase in Escherichia coli. The bacteria Bacteroides and bifid bacteria are the most typical representatives of anaerobic bacteria, whereas the bacteria Escherichia coli are the typical representatives of aerobic bacteria. According to the literature, Bacteroides and bifid bacteria reveal an intense fluorescence. Bearing in mind that these genera of bacteria play an important role in the gastrointestinal microbiocenosis, fluorescence spectroscopy can be used as a diagnostic means for gastrointestinal dysbacteriosis.
