[Evaluation of dipyridamole efficacy as an agent for preventing acute respiratory viral diseases]. / Otsenka éffektivnosti dipiridamola kak sredstva profilaktiki ostrykh respiratornykh virusnykh zabolevanii.

The epidemiological effectiveness of a low molecular interferon inducer, dipyridamole, as a means of prevention of influenza and ARD was studied in a double-blind epidemiological trial in a period of seasonal rise of ARD (Dec., 26, 1984-March 13, 1985). A statistically significant decrease of ARD incidence (1.91-fold) in the experimental group was confirmed by the results of serological studies and titrations of serum interferon in paired sera collected before the prevention and 10 days after its termination in selected subgroups from the experimental and control groups. The results indicate that the use of dipyridamole may be effective for mass prevention of influenza and ARD.

[Use of immunoenzyme analysis employing a staphylococcal protein A conjugate with peroxidase in the serodiagnosis of influenza]. / Primenenie immunofermentnogo analiza s ispol'zovaniem kon''iugata belka A stafilokokka s peroksidazoi v serodiagnostike grippa.

A test-system was developed on the basis of solid-phase enzyme-immunoassay using protein A/peroxidase conjugate for the determination of antibody levels to influenza virus in sera of humans who had experienced a natural infection or received a live influenza vaccine. The accurate observation of the test conditions was demonstrated to give the results well correlating with those of the HI test. The use of isolated hemagglutinin as the antigen considerably increased the specificity of the enzyme-immunoassay and in a number of cases detected a 4-fold or higher rise of antibody titres to hemagglutinin in paired sera of the vaccinees where the HI test showed no rise in antibody titres.

[Epidemiological trial of the prophylactic effectiveness of the interferon inducer dipyridamole with respect to influenza and acute respiratory diseases]. / Epidemiologicheskoe ispytanie profilakticheskoi éffektivnosti induktora interferona dipiridamola v otnoshenii grippa i ostrykh respiratornykh zabolevanii.

The epidemiological effectiveness of dipyridamol, an interferon-inducing agent used for the prevention of influenza and viral acute respiratory diseases, was tested in 4 epidemiological trials, 3 of them carried out as double blind trials. Observations were made in groups of adults (a research institute, a factory) and children (a kindergarten, a school), comprising 1040 subjects in the test groups and 771 subjects in the control groups. The drug was used during the whole epidemic period (January--March 1983) according to the following schedule: 1 oral administration in 8 days, in doses of 8 mg for adults, 50 mg for schoolchildren and 24 mg for children in the kindergarten. The epidemiological effectiveness of the drug was evaluated by comparing the total morbidity rates in influenza and acute respiratory diseases in the test and control groups. The results of 4 trials showed a pronounced epidemiological effectiveness of dipyridamol. The values of the epidemiological effectiveness index of the drug were 2.38 in the kindergarten, 1.55 at the school, 7.42 at the factory and 2.16 at the research institute. The results of the study of dipyridamol suggest that further investigations should be made with a view to use it for the mass prevention of influenza and acute respiratory diseases.

[Modification of sulfhydryl groups in ribosomal proteins by a dinitrophenyl hapten]. / Modifikatsiia sul'fgidril'nykh grupp ribosomnykh belkov dinitrofenil'nym gaptenom.

A dinitrophenyl hapten capable of protein SH-group modification was synthesized and the specificity of its reaction with SH-groups of the E. coli ribosomal proteins was studied. The possibility of incorporation of the Dnp-modified protein into ribosomal subunits by in vitro reconstitution was demonstrated with the ribosomal protein S12. The Dnp-hapten attached to the protein S12 was found to be accessible for interaction with the Dnp-specific antibodies and therefore to be exposed on the surface of the reconstituted 30S subunit. Thus, the approach for incorporation of the antigenic groups into the protein components of supramolecular structures was proposed.