RESUMO
Direct expression of hepatitis B virus (HBV) surface antigen (HBsAg) gene under the control of the Escherichia coli tryptophan operon (trp) promoter has been achieved. Synthesis of HBsAg (both complete and lacking its N-terminal segment) as a part of hybrid proteins with the N-terminal portion coded by genes cat, kan or bla is controlled by the appropriate promoters, as well as by the trp promoter. The highest levels of expression, including those for direct synthesis of HBsAg, provide the accumulation of about 10(5) polypeptide molecules per bacterial cell.
Assuntos
Escherichia coli/genética , Genes Virais , Antígenos de Superfície da Hepatite B/genética , Clonagem Molecular , Escherichia coli/crescimento & desenvolvimento , Escherichia coli/imunologia , Regulação da Expressão Gênica , Antígenos de Superfície da Hepatite B/isolamento & purificação , Óperon , PlasmídeosRESUMO
A plasmid carrying the complete genome of hepatitis B virus (HBV) inserted into the BamHI site of the pBR322 plasmid vector has been constructed. The physical map of HBV DNA established for 13 restriction endonucleases allows to conclude that the cloned DNA is similar, but not identical to the HBV DNA of ayw subtype.
