RESUMO
Bovine factor H was found to be polymorphic by the combined techniques of SDS-polyacrylamide electrophoresis of bovine plasma and immunoblotting. Three phenotypes (S, SF, F) were identified in a sample population of 149 cattle. Variant S and F differed by an apparent molecular weight of 5000 daltons. Family studies demonstrated Mendelian segregation of variants S and F. The data indicate that these genetic variants of bovine factor H are encoded by two codominant alleles at a single autosomal locus.
Assuntos
Bovinos/genética , Proteínas Inativadoras do Complemento C3b/genética , Polimorfismo Genético , Alelos , Animais , Proteínas Inativadoras do Complemento C3b/análise , Fator H do Complemento , Eletroforese em Gel de Poliacrilamida , Feminino , Frequência do Gene , Immunoblotting , Masculino , Peso MolecularRESUMO
A single embryo obtained from a superovulated Holstein-Friesian cow resulted in female twins following transfer.Blood typing studies including 17 genetic systems showed that the twins had identical genotypes. The probability that dizygous female twins from the same parents have identical blood types by chance was calculated as 1.34 x 10(-7).
RESUMO
Este trabajo consiste en una revision bibliografica actualizada en lo referente a grupos sanguineos en ganado bovino. El trabajo se ha resumido en 5 capitulos: I) Introduccion historica, II) Serologia,III) Genetica-Inmunogenetica, IV) Aplicaciones practicas de los grupos sanguineos y V) Correlacion entre los grupos sanguineos y la produccion. Es la esperanza de que este trabajo contribuya a estimular futuras investigaciones en esta rama de la Inmunogenetica, la que hasta el presente ha sido descuidada en el pais
Assuntos
Animais , Sistema ABO de Grupos Sanguíneos , BovinosAssuntos
Antígenos de Grupos Sanguíneos , Bovinos/genética , Animais , Bovinos/sangue , Quimera , Transferência Embrionária , Feminino , Masculino , Gravidez , Transplante Homólogo , GêmeosRESUMO
Samples of homozygous bovine serum transferrins have been prepared and their purity has been ascertained by immunological techniques and electrophoretic analysis in SDS. Measurements of carbohydrate composition show that no significant differences exist among the phenotype variants AA, D1D1, D2D2, and EE. Chromatography of transferrin AA on DEAE-cellulose separated four subfractions, each of which corresponded well with one band obtained by polyacrylamide gel electrophoresis. Carbohydrate analyses of the individual subfractions did not show significant differences in sialic acid, hexose, or hexosamine contents. After desialylation with neuraminidase, each subfraction was converted to a major band and a minor band on gel electrophoresis. From the relative band positions of the desialylated transferrins, it was concluded that possession of sialyl residued by bovine transferrin is not the primary cause of electrophoretic multiplicity. Rather, sialic acid masks an underlying heterogeneity which most likely resides within the polypeptide chain. Further characterization of this heterogeneity will best be undertaken with the isolated asialotransferrin subfractions.
