1.
Vision Res
; 46(27): 4547-55, 2006 Dec.
Artigo
em Inglês
| MEDLINE
| ID: mdl-17014882
RESUMO
Metarhodopsin-II is the light-excited form of rhodopsin that triggers G protein function. Metarhodopsin-II is stabilized when the N-terminus of the carboxyl (340-350) tail peptide of the alpha-subunit of transducin (Gtalpha) is crosslinked to rhodopsin cysteine 140 or the 340-350 peptide C-terminus of Gtalpha is crosslinked to rhodopsin cysteine 316. When the N-terminus of the peptide is coupled to C316 the MI<-->MII equilibrium is not affected. The evidence suggests that the N-terminus of the 340-350 region of Gtalpha is located near C140 when transducin stabilizes metarhodopsin-II and alternative explanations are suggested for the effectiveness of the 340-350 Gtalpha tail peptide when bound to C316.