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1.
Food Microbiol ; 59: 142-9, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27375255

RESUMO

The objective of this study was to evaluate the possibility of using polyphenolic extracts from cherry and blackcurrant leaves as natural antimicrobial agents in meat products. The polyphenolic composition of the extracts was analyzed and their impact on the microbial quality, lipid oxidation, color, and sensory evaluation of pork sausages was studied. Polyphenolic extracts were obtained from leaves collected in September. The total polyphenolic content in sour cherry leaf extract was 1.5 times higher than that found in blackcurrant leaf extract. Analysis of the polyphenol profile of each extract revealed two major groups: phenolic acids and flavonoids, including epigallocatechin and glycosides of quercetin and kaempferol. After chilling the sausages for 14 and 28 days, the extracts caused significantly lower MDA generation, indicating an antioxidant effect. Color changes after 28 days of storage were perceptible in the case of all treatments, with and without polyphenols. The application of sour cherry and black currant leaf extracts increased the shelf life of vacuum-packed sausages. Both extracts enhanced the microbial quality of the pork sausages over 14 days of refrigerated storage. Sour cherry leaf polyphenols were more effective against almost all studied groups of microorganisms.


Assuntos
Conservantes de Alimentos/farmacologia , Produtos da Carne/microbiologia , Extratos Vegetais/farmacologia , Folhas de Planta/química , Polifenóis/farmacologia , Prunus avium/química , Animais , Catequina/análogos & derivados , Catequina/farmacologia , Cromatografia Líquida de Alta Pressão , Conservantes de Alimentos/química , Armazenamento de Alimentos , Quempferóis/farmacologia , Produtos da Carne/análise , Consórcios Microbianos/efeitos dos fármacos , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Polifenóis/análise , Polifenóis/isolamento & purificação , Quercetina/farmacologia , Ribes/química , Suínos
2.
Food Microbiol ; 32(1): 212-6, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22850396

RESUMO

The objective of the study was to determine the Minimal Inhibitory Concentration (MIC) of thyme (29.4% thymol, 21.6% p-cymene) and rosemary essential oils (27.6% 1,8-cineole, 13.5% limonene, 13.0% ß-pinene) against Brochothrix thermosphacta and to establish the feasibility of their use as components of modified atmosphere during beef refrigerated storage. The minimum inhibitory concentration (MIC) of thyme oil against B. thermosphacta is 0.05% and that of rosemary oil 0.5%. The MIC values are independent on strain and temperature of growth, however the bactericidal effects are strain dependent. The addition of any of oil at a concentration equal to 2MIC to the modified atmosphere (80% O(2)/20% CO(2)) does not significantly influence the microbial quality of meat. At the same time, such a concentration of the essential oils was considerably detrimental to the organoleptic factors.


Assuntos
Brochothrix/efeitos dos fármacos , Conservação de Alimentos/métodos , Carne/microbiologia , Viabilidade Microbiana/efeitos dos fármacos , Óleos Voláteis/farmacologia , Óleos de Plantas/farmacologia , Rosmarinus/química , Thymus (Planta)/química , Animais , Brochothrix/crescimento & desenvolvimento , Bovinos , Armazenamento de Alimentos , Carne/análise , Testes de Sensibilidade Microbiana , Óleos Voláteis/química , Oxigênio/análise , Óleos de Plantas/química
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