RESUMO
AIM: Analysis of genetic heterogeneity of Pseudomonas aeruginosa strains isolated in and out of hospitals. MATERIALS AND METHODS: To study the genetic diversity of 36 strains of P. aeruginosa plasmid analysis, random amplified polymorphic DNA (RAPD) technique as well as polymerase chain reaction for detection of virulence genes algD, lasB, toxA, plcH, plcN, exoS, nan1, and nan2. RESULTS: Epidemically important strains were found in different ecological niches. It was shown that these virulence factors could play important roles in pathogenesis of infection. CONCLUSION: RAPD technique was effective for analysis of P. aeruginosa isolates. Number of studied typing bands differed between related isolates for each random primer.
Assuntos
Infecções por Pseudomonas/epidemiologia , Pseudomonas aeruginosa/genética , Primers do DNA , Humanos , Plasmídeos/genética , Reação em Cadeia da Polimerase , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/classificação , Pseudomonas aeruginosa/isolamento & purificação , Técnica de Amplificação ao Acaso de DNA Polimórfico , Federação Russa/epidemiologia , Sensibilidade e Especificidade , População Urbana , Fatores de Virulência/genéticaRESUMO
Analyzing many year's data on the prevalence of giardiasis among Volgograd dwellers indicated that the five-year infectivity index did not substantially change and it was 6.3 +/- 0.4%. The rate of Giardia lamblia isolation was higher in healthy individuals in the high-loaded environmental topodemic regions of the city than in those in the good ones. Over 50% of all the cases of giardiasis were recorded among 7-15-year-old children, the proportion of preschool children was much less (31.9%). The rate of giardiasis detection in children who went to children's preschool facilities was twice higher than that in the unorganized population. The focality index was 6.8%, which is indicative of the low contagiousness of the disease.
