Preparation of mixed keratinocyte and melanocyte cultures from biopsy specimens of pigmented skin sites of patients with vitiligo.

A method for preparation and expansion of mixed keratinocyte and melanocyte culture from human skin biopsy specimens was developed. The culture contains two melanocyte types: derivatives of hair follicle stem cells and epidermal basal layer stem cells. Fibroblasts were completely eliminated after culturing in selective medium.