[Tests of some peptides isolated from residue after insulin isolation from the pancreas. II. Establishment of heterogeneity level]. / Badania niektórych peptydów wyodrebnionych z pozostalosci po izolowaniu insuliny z trzustki. II. Ustalanie stopnia heterogenicznosci.

Heterogeneity of the active substance of Lipotropin-Polfa preparation was investigated by the method of gel column chromatography. The isolated polypeptide hormone has been found to possess the average molecular weight nine times smaller than that reported in the literature. This polypeptide was separated into six fractions by chromatography on Sephadex G-10. These fractions were found to differ in respect of the qualitative and quantitative amino acid composition. With the purpose of comparison, an analogous study of Lipormone-Labor. Choay (France) was performed. Similarity of this preparation to fraction I of Lipotropin-Polfa has been shown.

[Study of various peptides isolated from residues after extraction of insulin from the pancreas. I. Chemical characteristics of the studied substances]. / Badanie niektórych peptydów wyodrebnionych z pozostalosci po izolowaniu insuliny z trzustki. I. Charakterystyka chemiczna badanego materialu.

Several series of Lipotropin substance produced at "Polfa" were subjected to physiochemical investigations. The contents of dry residue, ash, ribose, protein, total nitrogen, ammonium nitrogen and amino acid nitrogen were determined. Particular series proved to differ in contents of ribose and amino acids. For the purpose of comparison, identical studies were carried out with Lipormone made by "Labor. Choay" (France). Substantial differences were found between the two preparations.