[Efficiency of hydroxyethyl starch use for arterial hypotension and shock in early postoperative period].

UNLABELLED: It is common knowledge that arterial hypotension is a one of most prevalent pathology of early neonatal period and it causes severe neurological complications. Purpose of the prospective randomized open study was to assess the efficacy of HES 130/0.4 (6% Voluven) as a start medicine for arterial hypotension in early neonatal period in comparison with normal saline solution (0.9% NaCl). MATERIALS AND METHODS: the study was held from January 2010 to September 2011. Newborns of the ICU with arterial hypotension on the first day of life were included into the study. Acute haemorrhage was an exclusion criterion. Routine monitoring of arterial pressure, heart rate and diuresis was carried out in all newborns. pH, pCO2, pO2 blood glucose, lactate and BE levels were measured before and after the treatment. Echocardiography, examination of regional blood flow in anterior cerebral artery, renal artery and mesenteric artery was held HES 130/0.4 (6% Voluven) and saline solution (0.9% NaCl) were used Parameters of haemostasis, biochemical and haematological analysis monitored after the solutions use. Time of arterial pressure normalization and duration of the treatment positive effect were recorded Episodes of hypotension, amount of cardiotonics per day and per 7 days and duration of cardiotonics administration were recorded as well. RESULTS: 6% Voluven infusion both to 0.9% NaCl infusion normalizes the regional circulation. Furthermore it increases the blood pH and diuresis. Lactate level normalization occurred only after 6% Voluven infusion. There was not change of serum sodium level. Liver enzymes, C-protein, prothrombin index were same in both groups of patients. 6% Voluven use in newborns with extremely low birth weight was accompanied with increasing of creatinine level, prolongation of activated partial thromboplastin time and increasing of intraventricular hemorrhage rate. Newborns with weight over 1000 gram did not have these complications. Voluven advantage in comparison with saline solution is a decreasing of cardiotonics administration in patients with weight over 1000 gram. CONCLUSIONS: 6% Voluven is more effective than 0.9% NaCl for increasing of cardiac output, myocardial contractile ability and diuresis, normalization of liquid balance and lactate level, decreasing of cardiotonics amount. 6% Voluven can be recommended for arterial hypotension treatment in newborns with weight over 1000 gram both to normal saline solution especially in case of shock.

[Effect of surface-active substances on hydroxylase activity of Tieghemella orchidis in a free and immobilized state]. / Izuchenie deistviia poverkhnostno-aktivnykh veshchestv na gidroksilaznyiu aktivnost' kletok Tieghemella orchidis v svobodnom i immobilizovannom sostoianii.

The effect of nonionogenic surface-active substances (SAS) on hydroxylase activity of Tieghemella orchidis in free and polyacrylamide gel (PAAG) immobilized states was studied. It was shown that addition of SAS (1%) to the cultivation medium simultaneously with the inoculation stimulated the filamentous growth of the mycelium. It was also demonstrated that certain SAS (span-80/sorbitanemonooleate/and polyethylene glycol-600-monolaurate) affected beneficially the culture transformation activity. The hydroxylase activity also increased after mycelium preincubation with tweens (1%) and transformation in the presence of span-20 (sorbitane monolaurate) and span-80 (0.3%). The use of the latter substances during gel preincubation with immobilized cells led to the stabilization of transformation activity, increase in the hydroxylation rate, and a change in the yield of hydroxylated products to produce hydrocortisone.

[20alpha- and 20beta-reduction of steroids by immobilized enzyme preparations and Bacillus megaterium cells]. / Izuchenie 20alpha- i 20beta-vosstanovleniia steroidov immobolizovannymi fermentnymi preparatami i kletkami Bacillus megaterium?

Cells of Bacillus megaterium immobilized due to incorporation into polyacrylamide gel retained their viability. They differed significantly in their enzymic activity from free cells: 20alpha- and 20beta-hydroxysteroid dehydrogenase activites were substantially decreased and they also showed the capacity to synthesize significant quantities of a new derivative and to destruct steroids. Immobolized protein fractions maintained 20alpha-hydroxysteroid dehydrogenase and 20beta-hydroxysteroid dehydrogenase activities but their specific activites were lower than those of native protein fractions. It is suggested that the synthesis of new compounds was induced by acrylamide that was bound during polymerization.