Assessment of estrogen receptor gene polymorphism (t-397c variant) in patients with premenstrual syndrome.

OBJECTIVE: The aim:of the study is to determine the frequency of polymorphism of estrogen receptor gene ESR1 (T-397C variant) in patients with premenstrual syndrome. PATIENTS AND METHODS: Materials and methods: 50 women with diagnosis of premenstrual syndrome (the basic group) and 25 persons without it (the control group) were examined. Polymerase chain reaction was used to study T-397C polymorphism of estrogen receptor gene ESR1. RESULTS: Results:There was no significant difference in allele and genotype rates of ESR1 gene between persons with premenstrual syndrome and controls. TT genotype was determined in 24.0 % women in the control group and 24 % of patients in basic group (OR=1.00, 95 % CI=0.32-3.08, p=1.00), TC genotype - in 52.0 % and 46.0 % of individuals respectively (OR=0.79, 95 % CI=0.30-2.06, p=0.62), CC genotype - 24.0 % and 30.0 % of women respectively (OR=1.36, 95 % CI=0.45-4.07, p=0.59). Also, the frequency of T allele and C allele was similar in individuals with pathology and healthy women. There was no significant difference in allele and genotype rates of T-397C variant of ESR1 gene between patients with mild and severe forms of premenstrual syndrome and controls. CONCLUSION: Conclusions: There is no association of T-397C polymorphic variant of estrogen receptor gene ESR1 with the development of premenstrual syndrome.

ESTRADIOL BLOOD LEVEL AND ESR1 GENE POLYMORPHISM IN WOMEN WITH PREMENSTRUAL SYNDROME.

OBJECTIVE: The aim: To evaluate the association between estrogen receptor (ESR1) α- Xbal polymorphism with estradiol serum blood level in the patients with premenstrual syndrome. PATIENTS AND METHODS: Materials and methods: 50 women with premenstrual syndrome and 20 controls were examined. The level of estradiol was measured in the blood serum in both phases of the menstrual cycle by ELISA method. Polymerase chain reaction was used to study ESR1 gene polymorphism (A-351G variant). RESULTS: Results: The estradiol concentration was similar in two phases of the menstrual cycle between healthy women and patients with premenstrual syndrome. But the more growth of estradiol in the luteal phase was determined in the persons with premenstrual syndrome. The rate of GG genotype was the largest in women with severe premenstrual syndrome (χ2=3.52, p=0.06). Also, in the persons with severe premenstrual syndrome who had G allele (GG+AG genotype) the estradiol concentration in the luteal phase of the menstrual cycle was on 50.00 % (p=0.02) higher compared to carriers of AA genotype. There was no difference in estradiol level between healthy women with GG+AG genotype and AA genotype. CONCLUSION: Conclusions: AG polymorphism of ESR1 gene may be the marker of development of premenstrual syndrome.