Formation of MnO2-coated ITO electrodes with high catalytic activity for enzymatic glucose detection.

We present the formation of a cheap and environmentally friendly working electrode material for glucose biosensors with good catalytic properties. The classic electrode in such devices consists of a conductive material modified with the enzyme glucose oxidase. The working principle is the electrochemical detection of hydrogen peroxide as a product of the enzymatic transformation of glucose. As a base material, we offer manganese dioxide; it is a natural highly selective catalyst for the decomposition of H2O2 and is electrochemically deposited onto the surface of ITO. We approached the formation of MnO2 films systematically. By changing parameters such as the deposition method, pH of the electrolyte, and the drying temperature of the precipitate, a series of electrodes were formed. These electrodes were characterized by SEM, electrochemical impedance spectroscopy, and XPS and their electrocatalytic activity was studied. Significant differences in the sensitivity of the electrodes were detected. The manganese dioxide film with the best catalytic characteristics is formed in the electrolyte with pH 1 by cyclic voltammetry and then drying at 60 °C. The surface of the electrode was then modified with a solution of GOx enzyme with a concentration of 2 mg ml-1 (100-250 units per mg solid). The sensitivity of such an electrode is 117.8 µA mmol-1 cm-2. The range of determined concentrations of glucose is from 0.1 mM to 3 mM. The sensitivity is comparable to that of electrodes based on expensive materials such as graphene and noble metals.

Materials characterization of TiO2 nanotubes decorated by Au nanoparticles for photoelectrochemical applications.

The structural and chemical modification of TiO2 nanotubes (NTs) by the deposition of a well-controlled Au deposit was investigated using a combination of X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), Scanning Transmission Electron Microscopy (STEM), Raman measurements, UV-Vis spectroscopy and photoelectrochemical investigations. The fabrication of the materials focused on two important factors: the deposition of Au nanoparticles (NPs) in UHV (ultra high vacuum) conditions (1-2 × 10-8 mbar) on TiO2 nanotubes (NTs) having a diameter of ∼110 nm, and modifying the electronic interaction between the TiO2 NTs and Au nanoparticles (NPs) with an average diameter of about 5 nm through the synergistic effects of SMSI (Strong Metal Support Interaction) and LSPR (Local Surface Plasmon Resonance). Due to the formation of unique places in the form of "hot spots", the proposed nanostructures proved to be photoactive in the UV-Vis range, where a characteristic gold plasmonic peak was observed at a wavelength of 580 nm. The photocurrent density of Au deposited TiO2 NTs annealed at 650 °C was found to be much greater (14.7 µA cm-2) than the corresponding value (∼0.2 µA cm-2) for nanotubes in the as-received state. The IPCE (incident photon current efficiency) spectral evidence also indicates an enhancement of the photoconversion of TiO2 NTs due to Au NP deposition without any significant change in the band gap energy of the titanium dioxide (E g ∼3.0 eV). This suggests that a plasmon-induced resonant energy transfer (PRET) was the dominant effect responsible for the photoactivity of the obtained materials.

Surface Characterization of MoS2 Atomic Layers Mechanically Exfoliated on a Si Substrate.

Mo disulfide overlayers with the thickness exceeding 1.77 nm were obtained on Si substrates through mechanical exfoliation. The resulting Mo disulfide flakes were then analyzed ex situ using combination of Auger electron spectroscopy (AES), elastic-peak electron spectroscopy (EPES) and scanning electron microscopy (SEM) in order to characterize their surface chemical composition, electron transport phenomena and surface morphology. Prior to EPES measurements, the Mo disulfide surface was sputter-cleaned and amorphized by 3 kV argon ions, and the resulting S/Mo atomic ratio varied in the range 1.80-1.88, as found from AES measurements. The SEM images revealed single crystalline small-area (up to 15 µm in lateral size) Mo disulfide flakes having polygonal or near-triangular shapes. Such irregular-edged flakes exhibited high crystal quality and thickness uniformity. The inelastic mean free path (IMFP), characterizing electron transport, was evaluated from the relative EPES using Au reference material for electron energies E = 0.5-2 keV. Experimental IMFPs, λ, determined for the AES-measured surface compositions were approximated by the simple function λ = kEp, where k = 0.0289 and p = 0.946 were fitted parameters. Additionally, these IMFPs were compared with IMFPs resulting from the two methods: (i) present calculations based on the formalism of the Oswald et al. model; (ii) the predictive equation of Tanuma et al. (TPP-2M) for the measured Mo0.293S0.551C0.156 surface composition (S/Mo = 1.88), and also for stoichiometric MoS2 composition. The fitted function was found to be reasonably consistent with the measured, calculated and predicted IMFPs. We concluded that the measured IMFP value at 0.5 keV was only slightly affected by residual carbon contamination at the Mo disulfide surface.

Plasma Nitriding of TiO2 Nanotubes: N-Doping in Situ Investigations Using XPS.

The nitrogen doping of titanium dioxide nanotubes (TiO2 NTs) was investigated as a result of well-controlled plasma nitriding of TiO2 NTs at a low temperature. This way of nitrogen doping is proposed as an alternative to chemical/electrochemical methods. The plasma nitriding process was performed in a preparation chamber connected to an X-ray photoelectron spectroscopy (XPS) spectrometer, and the nitrogen-doped TiO2 NTs were next investigated in situ by XPS in the same ultrahigh vacuum (UHV) system. The collected high-resolution (HR) XPS spectra of N 1s, Ti 2p, O 1s, C 1s, and valence band (VB) revealed the formation of chemical bonds between titanium, nitrogen, and oxygen atoms as substitutional or interstitial species. Moreover, the results provided a characterization of the electronic states of N-TiO2 NTs generated by various plasma nitriding and annealing treatments. The VB XPS spectrum showed a reduction in the TiO2 band gap of about 0.6 eV for optimal nitriding and heat-treated conditions. The TiO2 NTs annealed at 450 or 650 °C in air (ex situ) and nitrided under UHV conditions were used as reference materials to check the formation of Ti-N bonds in the TiO2 lattice with a well-defined structure (anatase or a mixture of anatase and rutile). Scanning electron microscopy microscopic observations of the received materials were used to evaluate the morphology of the TiO2 NTs after each step of the nitriding and annealing treatments.

[Estimation of transmission hazard of Leptospira Sp. infections in 2 groups of people]. / Ocena ryzyka transmisji zakazen Leptospira sp. w dwóch grupach ludzi.

The aim of the study was to determine the possible risk of the leptospiral transmission from animals to humans. 457 humans (both men and women) were divided into 2 groups: 1) of possible high risk infection consisted of people dealing as farm workers with cattle and pigs 2) of possible low risk infection consisted of people selected randomly. The animals on the farms were previously tested and found positive. All sera were examined using micro-agglutination test (MAT) with a battery of 18 serovars. The statistical evaluation of the results was performed using chi 2 test. The infection rate in I group was 13.79% and in II group was 1.5%. (p < or = 0.001). Sera of I group reacted with 7 serovars (sejroe, bratislava, canicola, tarassovi, bataviae, celledoni, patoc) and of II with 3 (hebdomadis, sejroe, hardjo). The infection rate in the group I was over 9 times higher than in group II. Thus dealing with infected animals is a high risk factor.