Characterization and prevalence of a polymorphism in the 3' untranslated region of cytochrome P4501A1 in cancer-prone Atlantic tomcod.

Atlantic tomcod (Microgadus tomcod) from the cancer-prone Hudson River population exhibit a genetic polymorphism in the cytochrome P4501A (CYP1A) gene which is evidenced in Northern blot analyses by a truncated transcript and in Southern blot analyses by a deletion in the variant allele. To initially evaluate the functional significance of this polymorphism, we sought to characterize the molecular basis for this polymorphism and to determine its frequency in tomcod from other populations in which the prevalence of neoplasia is low. The common CYP1A allelic sequence was determined from beta-naphthoflavone-induced tomcod cDNA and from tomcod genomic DNA. A sequence of the variant CYP1A allele was obtained by direct sequence analysis of the amplicons of variant tomcod cDNA and genomic DNA. CYP1A exon and intron structure is highly conserved between tomcod and all other teleost and mammalian species compared. Similarity of the deduced tomcod, rainbow trout, and plaice amino acid sequences was 72%, whereas similarity between tomcod and mammalian sequences was approximately 50%. The variant tomcod CYP1A allele results from a 606-bp deletion in the 7th exon of the 3' untranslated region (UTR) of the cDNA. Polymerase chain reaction and Northern blot analyses revealed an absence of this CYP1A polymorphism in tomcod from other rivers. Studies in humans suggest that variation in CYP1A1 cDNA may impact on genetic susceptibility to environmentally induced neoplasia. Furthermore, studies in in vitro mammalian models indicate the importance of 3' UTRs on gene expression by impacting on the stability of transcript. These results suggest that the 3' UTR CYP1A polymorphism in tomcod may have consequences for the genetic susceptibility of Hudson River fish to hepatic neoplasia.

A biomarker approach to assessing xenobiotic exposure in Atlantic tomcod from the North American Atlantic coast.

We determined levels of hepatic cytochrome P4501A (CYP1A) mRNA, hepatic DNA adducts, and fluorescent aromatic compounds (FACs) in bile, a measure of exposure to polyaromatic hydrocarbons, in Atlantic tomcod from six river systems ranging from highly polluted to relatively pristine on the northeast North American coast (the Hudson River, New York; the St. Lawrence River, Quebec; the Miramichi River, New Brunswick; the Saco and Royal rivers, Maine; and the Margaree River, Nova Scotia). Hudson River tomcod showed the greatest response for all parameters, and tomcod from the Margaree River exhibited the least response. Tomcod from the Miramichi River exhibited marked induction of CYP1A mRNA but low levels of hepatic DNA adducts and biliary FACs, whereas fish from the St. Lawrence River showed no induction of CYP1A mRNA and moderately elevated levels of DNA adducts and biliary FACs. In tomcod from the Hudson and Miramichi rivers, the levels of CYP1A mRNA were 28 times and 14 times, respectively, as great as the levels in fish from the St. Lawrence, Saco/Royal, and Margaree rivers. Mean levels of DNA adducts varied from 120 nmol adducts/mol bases in Hudson River tomcod to < 3 nmol adducts/mol bases in fish from the Miramichi and Margaree rivers. Concentrations of FACs in the bile of tomcod from the Hudson and St. Lawrence rivers were 8 and 1.8 times, respectively, as great as the concentrations in tomcod from the Miramichi River and Margaree River. In tomcod from the Hudson River, all three biomarkers were markedly elevated; in the St. Lawrence River two biomarkers were elevated, in the Miramichi River one was elevated, but no biomarker was substantially elevated in fish from the Saco/Royal and Margaree rivers. Elevated levels of hepatic DNA adducts and biliary FACs in tomcod from the Hudson River suggest increased exposure to PAHs, consistent with previous studies.

Cytochrome P450IA mRNA expression in feral Hudson River tomcod.

We sought to determine if levels of cytochrome P450IA gene expression are environmentally induced in feral populations of Hudson River tomcod, a cancer prone fish, and whether laboratory exposure of tomcod to artificially spiked and naturally contaminated Hudson sediments can elicit a significant response. Using Northern blot analysis, we found levels of P450IA mRNA in tomcod collected from two Hudson River sites higher than those in tomcod from a river in Maine. Depuration of environmentally induced Hudson tomcod P450IA mRNA was rapid, with an initial detectable decline in P450 gene expression by 8 hr and basal levels reached by 5 days. Intraperitoneal injection of beta-napthoflavone in depurated Hudson tomcod resulted in a 15-fold induction of P450 gene expression within 26 hr. Exposure of depurated Hudson tomcod to natural sediment spiked with two PAHs resulted in a 7-fold induction of P450 gene expression. Exposure of depurated tomcod to sediment from a contaminated Hudson site also resulted in a 7- to 15-fold induction of P450IA mRNA expression. Northern blot analysis revealed a second polymorphic cytochrome P450IA mRNA band in some tomcod which was also detected by Southern blot analysis. Induction of cytochrome P450IA mRNA in Atlantic tomcod may provide a sensitive biomarker of environmentally relevant concentrations of some pollutants in the Hudson and other northeastern tidal rivers.